Fluorescent pseudomonads pursue media-dependent strategies to inhibit growth of pathogenic Verticillium fungi

Nesemann, Kai; Braus‐Stromeyer, Susanna A.; Harting, Rebekka; Höfer, Annalena; Kusch, Harald; Ambrosio, Alinne Batista; Timpner, Christian; Braus, Gerhard H.

doi:10.1007/s00253-017-8618-5

Cited by 6 publications

(11 citation statements)

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“…Bacterial isolates from the rhizosphere were used for these experiments. The analyzed strains exhibited a stronger antagonistic potential toward V. longisporum compared to the lipopeptide producing bacteria ( Nesemann et al, 2018 ). The growth inhibition potential of the rhizosphere bacteria on plate was dependent on the medium and the genomic potential to produce different metabolites Pseudomonas fluorescens DSM8569 (P_rhizo) was isolated from the rhizosphere of rapeseed ( Berg and Ballin, 1994 ).…”

Section: Resultsmentioning

confidence: 99%

“…The co-cultivation experiments on solid SXM were performed as previously described in Hollensteiner et al (2017) and Nesemann et al (2018) . Spores of Verticillium spp.…”

Section: Methodsmentioning

confidence: 99%

“…Rhizosphere bacteria including the genera Bacillus or Pseudomonas reduce Verticillium spp. growth in co-culture with potential plant growth promoting or biocontrol functions ( Hayat et al, 2010 ; Hollensteiner et al, 2017 ; Nesemann et al, 2018 ). In general, bacteria can prevent pathogen infection by a variety of mechanisms.…”

Section: Introductionmentioning

confidence: 99%

“…Cyclic lipodepsipeptides as for instance syringomycin and syringopeptin can be inserted into plasma membranes to form ion channels resulting in ion leakage and cell death ( Hutchison and Gross, 1997 ). The inhibitory potential of bacteria in fungal co-cultures varies considerably in the presence of different nutrients affecting fungal spore germination or growth ( Michelsen and Stougaard, 2012 ; Nesemann et al, 2018 ). We could show that Pseudomonas isolates from soil are not only able to inhibit plant pathogenic Verticillium strains but also moderately affect growth of soil saprophytes of the genus Aspergillus ( Nesemann et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

“…The inhibitory potential of bacteria in fungal co-cultures varies considerably in the presence of different nutrients affecting fungal spore germination or growth ( Michelsen and Stougaard, 2012 ; Nesemann et al, 2018 ). We could show that Pseudomonas isolates from soil are not only able to inhibit plant pathogenic Verticillium strains but also moderately affect growth of soil saprophytes of the genus Aspergillus ( Nesemann et al, 2018 ). Aspergillus nidulans is an important and well-studied model organism and is widely distributed in the soil.…”

Section: Introductionmentioning

confidence: 99%

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Pseudomonas Strains Induce Transcriptional and Morphological Changes and Reduce Root Colonization of Verticillium spp.

et al. 2021

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Phytopathogenic Verticillia cause Verticillium wilt on numerous economically important crops. Plant infection begins at the roots, where the fungus is confronted with rhizosphere inhabiting bacteria. The effects of different fluorescent pseudomonads, including some known biocontrol agents of other plant pathogens, on fungal growth of the haploid Verticillium dahliae and/or the amphidiploid Verticillium longisporum were compared on pectin-rich medium, in microfluidic interaction channels, allowing visualization of single hyphae, or on Arabidopsis thaliana roots. We found that the potential for formation of bacterial lipopeptide syringomycin resulted in stronger growth reduction effects on saprophytic Aspergillus nidulans compared to Verticillium spp. A more detailed analyses on bacterial-fungal co-cultivation in narrow interaction channels of microfluidic devices revealed that the strongest inhibitory potential was found for Pseudomonas protegens CHA0, with its inhibitory potential depending on the presence of the GacS/GacA system controlling several bacterial metabolites. Hyphal tip polarity was altered when V. longisporum was confronted with pseudomonads in narrow interaction channels, resulting in a curly morphology instead of straight hyphal tip growth. These results support the hypothesis that the fungus attempts to evade the bacterial confrontation. Alterations due to co-cultivation with bacteria could not only be observed in fungal morphology but also in fungal transcriptome. P. protegens CHA0 alters transcriptional profiles of V. longisporum during 2 h liquid media co-cultivation in pectin-rich medium. Genes required for degradation of and growth on the carbon source pectin were down-regulated, whereas transcripts involved in redox processes were up-regulated. Thus, the secondary metabolite mediated effect of Pseudomonas isolates on Verticillium species results in a complex transcriptional response, leading to decreased growth with precautions for self-protection combined with the initiation of a change in fungal growth direction. This interplay of bacterial effects on the pathogen can be beneficial to protect plants from infection, as shown with A. thaliana root experiments. Treatment of the roots with bacteria prior to infection with V. dahliae resulted in a significant reduction of fungal root colonization. Taken together we demonstrate how pseudomonads interfere with the growth of Verticillium spp. and show that these bacteria could serve in plant protection.

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Section: Resultsmentioning

confidence: 99%

“…The co-cultivation experiments on solid SXM were performed as previously described in Hollensteiner et al (2017) and Nesemann et al (2018) . Spores of Verticillium spp.…”

Section: Methodsmentioning

confidence: 99%