2009
DOI: 10.1016/j.mimet.2009.02.011
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Fluorescent reporters for Staphylococcus aureus

Abstract: With the emergence of Staphylococcus aureus as a prominent pathogen in community and healthcare settings, there is a growing need for effective reporter tools to facilitate physiology and pathogenesis studies. Fluorescent proteins are ideal as reporters for their convenience in monitoring gene expression, performing host interaction studies, and monitoring biofilm growth. We have developed a suite of fluorescent reporter plasmids for labeling S. aureus cells. These plasmids encode either green fluorescent prot… Show more

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Cited by 175 publications
(163 citation statements)
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“…The following strains of S. aureus were used for the study: methicillin-susceptible S. aureus (MSSA) ATCC 25923, methicillin-resistant S. aureus (MRSA) clinical isolate TJU2, MRSA LAC (USA300), MSSA Xen 36 (Perkin-Elmer), and MSSA AH1710, a strain that constitutively expresses green fluorescent protein (GFP) that was used to visualize staphylococcal surface colonization (a kind gift from Alexander Horswill, University of Iowa [21]). In addition, Staphylococcus epidermidis strain ATCC 35984 was used in the disk diffusion assays.…”
Section: Methodsmentioning
confidence: 99%
“…The following strains of S. aureus were used for the study: methicillin-susceptible S. aureus (MSSA) ATCC 25923, methicillin-resistant S. aureus (MRSA) clinical isolate TJU2, MRSA LAC (USA300), MSSA Xen 36 (Perkin-Elmer), and MSSA AH1710, a strain that constitutively expresses green fluorescent protein (GFP) that was used to visualize staphylococcal surface colonization (a kind gift from Alexander Horswill, University of Iowa [21]). In addition, Staphylococcus epidermidis strain ATCC 35984 was used in the disk diffusion assays.…”
Section: Methodsmentioning
confidence: 99%
“…S. aureus, E. faecalis, L. monocytogenes, Y. enterocolitica, and P. aeruginosa 24-h biofilms were prepared in microplates at 37°C and in TSB as described above for B. thuringiensis 407 (strains are listed in Table S1). Disruption of S. aureus biofilms by biocides was tested using the fluorescent strain RN4220 pALC2084 expressing GFP (29). The resulting biofilms had a thickness of around 30 μm.…”
Section: Methodsmentioning
confidence: 99%
“…For live cell imaging, GFP expression in bacteria is used extensively and expression systems are available for many different bacterial species, including clinically relevant strains of Salmonella, Streptococcus, Listeria monocytogenes, Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli O157:H7 [41,[44][45][46][47]. Plasmid based gene expression is a well-established and long-used method in microbiology.…”
Section: Discussionmentioning
confidence: 99%