Fluorescent tamoxifen-encapsulated nanocapsules functionalized with folic acid for enhanced drug delivery toward breast cancer cell line MCF-7 and cancer cell imaging

Nankali, Ehsan; Shaabanzadeh, Masoud; Torbati, Maryam Bikhof

doi:10.1007/s00210-020-01825-1

Cited by 20 publications

(12 citation statements)

References 28 publications

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“…The drug loading, encapsulation efficiency, and total cumulative drug release from TMX/PLA/DPPE-PEG NCs were decreased compared with our previous study on TMX-loaded PLGA/DPPE-PEG nanocapsules (Nankali et al 2020). PLA and PLGA degrade by hydrolysis of their ester linkage in aqueous media.…”

Section: In Vitro Tmx Release Profilecontrasting

confidence: 63%

“…Accordingly, a comparison between the present study results and findings of our previous research revealed that the cytotoxic effect of loaded tamoxifen in folate-modified PLGA nanocapsules is approximately four times more than PLA/DPPE-PEG nanocapsules on MCF-7 cells. This increase in effectiveness may be related to folate receptor targeting on the cell surface of MCF-7 or the faster release of tamoxifen from PLGA nanocapsules than PLA (Nankali et al 2020). Although the drug release of PLGA nanocarriers occurs faster at acidic pH of cancer cells, high initial burst release of drug as one of the disadvantages of PLGA nanocarriers could result in more side effects (Lu et al 2019).…”

Section: Cytotoxicity Analysis Of Tmx-loaded Pla/dppe-peg Nanocapsulesmentioning

confidence: 99%

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Tamoxifen-loaded PLA/DPPE-PEG lipid-polymeric nanocapsules for inhibiting the growth of estrogen-positive human breast cancer cells through cell cycle arrest

2020

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The strategy of drug encapsulation with biocompatible polymeric nanocapsules could be a promising approach to improve the delivery of poorly water-soluble drugs for cancer therapy. The aim of this study was to investigate the drug delivery of tamoxifen from biocompatible nanocapsules made of poly (D,L-lactide) (PLA) c o r e a n d 1 , 2 -d i p a l m i t o y l -s n -g l y c e r o -3phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000] (DPPE-PEG) shell in the treatment of breast cancer.The analytical techniques such as FTIR, XRD, SEM, and TEM were used to investigate the structure of nanocapsules. Tamoxifen drug encapsulation efficiency and loading percentages as well as release kinetics at different pH values and temperatures were used to confirm the appropriate performance of these novel nanocapsules. The maximum tamoxifen release occurred at 40°C, pH = 4. Based on the SEM and TEM images, the sizes of nanocapsules were between 57 and 159 nm. In order to investigate the anticancer effects and cytotoxicity of encapsulated tamoxifen in PLA/DPPE-PEG nanocapsules, the tetrazolium-based colorimetric (MTT) assay and cell cycle arrest analysis on MCF-7 breast cancer cell were performed. Also, cell cycle-related gene expressions of p53 and p21 in treated MCF-7 cells with tamoxifen-loaded PLA/DPPE-PEG nanocapsules were evaluated. The results of MTT cell proliferation experiments exhibited an increase in the cytotoxicity of tamoxifen nanocapsules on MCF-7 cell line compared with free tamoxifen. The 1.2-fold increase in the G1 stage compared with the control group in the cell cycle arrest protocol as well as the notable overexpression of p53 and p21 genes suggested that these nanocapsules arrested cell cycle in MCF-7 cells.

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Section: In Vitro Tmx Release Profilecontrasting

confidence: 63%

Section: Cytotoxicity Analysis Of Tmx-loaded Pla/dppe-peg Nanocapsulesmentioning

confidence: 99%

Tamoxifen-loaded PLA/DPPE-PEG lipid-polymeric nanocapsules for inhibiting the growth of estrogen-positive human breast cancer cells through cell cycle arrest

2020

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“…2 b shows the X-ray diffraction diagram of TMX-FPGs. The absence of sharp peaks indicates the amorphous nature of the TMX-FPGs resulting from the loading of TMX on the nanocarriers and indicates the disappearance of the crystalline nature of graphene due to functionalization with mPEG2000 and FA [29,30].…”

Section: Xrd Characterizationmentioning

confidence: 99%

“…6 a, the effect of these compounds on the cancer MCF-7 cell line at all three times was signi cantly greater than that of healthy HDF cells, and this difference was greater for TMX-FPGs than for TMX-PGs nanoparticles and free TMX. This may be due to the effect of folic acid on the structure of the TMX-FPGs in targeting drug delivery to folate receptor-positive MCF-7 cells [30].…”

Section: In Vitro Cell Viability Analysismentioning

confidence: 99%

Folic Acid-decorated and PEGylated Graphene Quantum Dots as efficient Tamoxifen delivery system against breast cancer cells: in vitro studies

Amraee

Majd

Torbati

et al. 2023

Preprint

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Nano-drug delivery systems have provided a platform for improving the formulations of targeted cancer therapy. Graphene quantum dots (GQDs) possess potential properties for the targeted transport and tracking of anticancer medication to tumor cells. For this purpose, the synthesized GQDs were decorated with folic acid for better binding to folate-positive breast cancer cells and methoxy polyethylene glycol (mPEG2000) for a longer lifetime, and finally loaded with anticancer drug tamoxifen (TMX). The synthesized nano-drugs were characterized using FTIR, XRD, UV-vis, PL, HRTEM, FESEM, and DLS analytical devices. Based on the obtained data, the average hydrodynamic diameter of particles dissolved in water was 294.7 nm and the size of dehydrated particles was between 3 and 153 nm. PL data showed that prepared nanoparticles have an emission wavelength of 438 nm, which is in the blue fluorescent wavelength range, thus making these nanoparticles suitable for cell imaging. The encapsulation efficiency and loading percentage of tamoxifen in nanoparticles were calculated at about 52.5% and 30%, respectively, and cumulative drug release reached 89% within 42 hours. The cytotoxicity effects of nanoparticles on MCF-7 cell line were investigated. The targeted mPEG-GQDs-FA/TMX (TMX-FPGs) nano-drug was more toxic than free TMX drug on MCF-7 cells and had reduced side effects on healthy HDF cells. TMX-FPGs induced apoptosis cell death in cancer cells, significantly. The confocal cell imaging experiments showed that TMX-FPGs are appropriate for monitoring and targeting MCF-7 cancer cells. These data have approved the potency of TMX-FPGs against breast cancer cells.

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“…Over the last decades, nanoparticles have gained great attention in medical sciences [ 1 , 2 , 3 ] especially in the fields of imaging [ 4 , 5 , 6 , 7 , 8 , 9 , 10 ], sensing [ 11 , 12 , 13 , 14 ], gene delivery systems [ 15 , 16 , 17 , 18 , 19 , 20 , 21 ] and drug delivery [ 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ]. Several studies have focused on the development of polymeric nanoparticles as drug delivery systems.…”

Section: Introductionmentioning

confidence: 99%

Lyophilization of Nanocapsules: Instability Sources, Formulation and Process Parameters

Degobert

Aydin

2021

Pharmaceutics

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Polymeric nanocapsules have gained more and more interest in the medical sciences. Their core-shell structure offers numerous advantages, especially regarding their use as drug delivery systems. This review begins by presenting the different intrinsic sources of the instability of nanocapsules. The physical and chemical potential instabilities of nanocapsules reduce their shelf-life and constitute a barrier to their clinical use and to their commercialization. To overcome these issues, lyophilization is often used as a process of choice in the pharmaceutical industry especially when labile compounds are used. The state of the art of lyophilization nanocapsules is reviewed. The formulation properties and the process parameters are discussed for a complete understanding of their impact on the stability and storage of the final dried product. To assess the quality of the dried product, various characterization methods are also discussed.

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Fluorescent tamoxifen-encapsulated nanocapsules functionalized with folic acid for enhanced drug delivery toward breast cancer cell line MCF-7 and cancer cell imaging

Cited by 20 publications

References 28 publications

Tamoxifen-loaded PLA/DPPE-PEG lipid-polymeric nanocapsules for inhibiting the growth of estrogen-positive human breast cancer cells through cell cycle arrest

Tamoxifen-loaded PLA/DPPE-PEG lipid-polymeric nanocapsules for inhibiting the growth of estrogen-positive human breast cancer cells through cell cycle arrest

Folic Acid-decorated and PEGylated Graphene Quantum Dots as efficient Tamoxifen delivery system against breast cancer cells: in vitro studies

Lyophilization of Nanocapsules: Instability Sources, Formulation and Process Parameters

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