Fluorescent thermometer based on a quinolinemalononitrile copolymer with aggregation-induced emission characteristics

Abstract

An AIEgen-grafted copolymer P(NIPAM-co-EM) is successfully constructed with thermo-responsive LCST-featuring matrix PNIPAM for temperature detection.

Functional Polymer Systems with Aggregation-Induced Emission and Stimuli Responses

“…c The relative fluorescent intensity at 607 nm as a function of temperature. Reproduced from [ 58 ] with permission from the Royal Society of Chemistry. d Chemical structure of TPE-labeled nylon-6.…”

AIE-active non-conjugated poly(N-vinylcaprolactam) as a fluorescent thermometer for intracellular temperature imaging

“…13,14 Until now, although multitudinous AIE-active molecules have been designed and synthesized, very few have been integrated with the thermo-responsive system to construct a uorescent thermometer with AIE characteristics for detection and monitoring of the variation of intracellular temperature at the microscale. 15,16 Despite having reasonable signicance, the aforementioned polymeric uorescent thermometers have some inherent disadvantages: (i) a tedious multi-step synthetic procedure is needed to synthesize such systems; (ii) these AIE-active moieties with p-p conjugated structures might lead to a lot of restrictions for biological applications namely high cytotoxicity, acute inammation and serious immunogenicity. 17 These complications have motivated us to develop an alternative design that might overcome these bottlenecks of conjugated compounds.…”

A simple yet effective AIE-based fluorescent nano-thermometer for temperature mapping in living cells using fluorescence lifetime imaging microscopy

“…Fluorophores with aggregation-induced emission (AIE) properties are now widely used as biosensors. [20][21][22][23][24][25][26][27][28] The fluorescence turn-on property of these probes is largely attributed to the restriction of intramolecular motion. They are inherently sensitive to microenvironmental viscosity since the viscous environment results in reduced intramolecular motion that leads to enhanced fluorescence intensity and prolonged fluorescence lifetime of AIE molecules.…”

“…29,30 Several AIE-based PNIPAM fluorescent temperature sensors have been reported. [22][23][24][25][26][27][28] These AIE probes are excellent reporters; however, like other dye-PNIPAM sensors, considerable synthetic effort are needed for the fabrication of AIE-PNIPAM sensors and the application of these sensors in cells has not been well demonstrated yet.…”