Fluorescently-tagged magnetic protein nanoparticles for high-resolution optical and ultra-high field magnetic resonance dual-modal cerebral angiography

Mishra, Saket; Hermán, Péter; Crair, Michael C.; Constable, R. Todd; Walsh, John J.; Akif, Adil; Verhagen, Justus V.; Hyder, Fahmeed

doi:10.1039/d2nr04878g

Cited by 5 publications

(3 citation statements)

References 73 publications

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“…Alternatively, the green or red could be used for either neurons or astrocytes, leaving the other channel to probe cerebral microvessels by using plasma-borne fluorescent probes. 78 This type of multi-modal fluorescent imaging would require careful design of optical hardware to measure the CBV signal concurrently.…”

Section: Discussionmentioning

confidence: 99%

Spatiotemporal features of neurovascular (un)coupling with stimulus-induced activity and hypercapnia challenge in cerebral cortex and olfactory bulb

James

Sanggaard

Akif

et al. 2023

J Cereb Blood Flow Metab

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Carbon dioxide (CO2) is traditionally considered as metabolic waste, yet its regulation is critical for brain function. It is well accepted that hypercapnia initiates vasodilation, but its effect on neuronal activity is less clear. Distinguishing how stimulus- and CO2-induced vasodilatory responses are (dis)associated with neuronal activity has profound clinical and experimental relevance. We used an optical method in mice to simultaneously image fluorescent calcium (Ca2+) transients from neurons and reflectometric hemodynamic signals during brief sensory stimuli (i.e., hindpaw, odor) and CO2 exposure (i.e., 5%). Stimuli-induced neuronal and hemodynamic responses swiftly increased within locally activated regions exhibiting robust neurovascular coupling. However, hypercapnia produced slower global vasodilation which was temporally uncoupled to neuronal deactivation. With trends consistent across cerebral cortex and olfactory bulb as well as data from GCaMP6f/jRGECO1a mice (i.e., green/red Ca2+ fluorescence), these results unequivocally reveal that stimuli and CO2 generate comparable vasodilatory responses but contrasting neuronal responses. In summary, observations of stimuli-induced regional neurovascular coupling and CO2-induced global neurovascular uncoupling call for careful appraisal when using CO2 in gas mixtures to affect vascular tone and/or neuronal excitability, because CO2 is both a potent vasomodulator and a neuromodulator.

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Section: Discussionmentioning

confidence: 99%

Spatiotemporal features of neurovascular (un)coupling with stimulus-induced activity and hypercapnia challenge in cerebral cortex and olfactory bulb

James

Sanggaard

Akif

et al. 2023

J Cereb Blood Flow Metab

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“…ESIONPs have shown the great application values in magnetic resonance imaging (MRI) due to their unique properties, such as switchable contrast signals and high biocompatibility (Kim et al, 2011;Shen et al, 2017;Cao et al, 2020). In addition, ESIONPs are also used as highly sensitive probes for detecting tumors and other lesions (Groult et al, 2021;Mishra et al, 2022;Zhang et al, 2022). As a component of nanotechnology, the application of ESIONPs in different fields is rapidly increasing, and understanding their potential cytotoxicity and mechanism is crucial for the safety of their application (Mohammadinejad et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptomics revealed neurodevelopmental impairments and ferroptosis induced by extremely small iron oxide nanoparticles

Lyu,

Kou,

et al. 2024

Front. Genet.

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Iron oxide nanoparticles are a type of nanomaterial composed of iron oxide (Fe3O4 or Fe2O3) and have a wide range of applications in magnetic resonance imaging. Compared to iron oxide nanoparticles, extremely small iron oxide nanoparticles (ESIONPs) (∼3 nm in diameter) can improve the imaging performance due to a smaller size. However, there are currently no reports on the potential toxic effects of ESIONPs on the human body. In this study, we applied ESIONPs to a zebrafish model and performed weighted gene co-expression network analysis (WGCNA) on differentially expressed genes (DEGs) in zebrafish embryos of 48 hpf, 72 hpf, 96 hpf, and 120 hpf using RNA-seq technology. The key hub genes related to neurotoxicity and ferroptosis were identified, and further experiments also demonstrated that ESIONPs impaired the neuronal and muscle development of zebrafish, and induced ferroptosis, leading to oxidative stress, cell apoptosis, and inflammatory response. Here, for the first time, we analyzed the potential toxic effects of ESIONPs through WGCNA. Our studies indicate that ESIONPs might have neurotoxicity and could induce ferroptosis, while abnormal accumulation of iron ions might increase the risk of early degenerative neurological diseases.

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“…Numerous types of DDSs have been employed, such as polymers [ 9 , 10 ], lipid nanoparticles (NPs) [ 11 ], and metallic NPs [ 12 ] (silver, gold, or magnetic NPs). Among these, magnetic NPs represent a promising alternative, due to their properties such as high stability, high saturation magnetization/large magnetic moment of particles, good response to moderate magnetic fields, inherent ability to cross biological barriers, protection of the drug from rapid degradation in biological systems, provision of a large surface area for conjugating targeting ligands [ 7 , 8 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ], low production costs [ 20 ], and superparamagnetism, which allows their guidance in the organism using an external magnetic field [ 7 , 21 ]. Scientific interest in NPs in general and in magnetic nanoparticles (MNPs) in particular has grown exponentially in the last decade, due to recent high-interest research on their properties and the fact that in a relatively short time, these materials have become particularly important tools in high-interest biomedical areas such as biomaterial science, biochemistry, diagnostics, magnetic drug and gene delivery, hyperthermia, magnetic resonance imaging (MRI), and theragnostics [ 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in the Development of Drug Delivery Applications of Magnetic Nanomaterials

et al. 2023

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With the predicted rise in the incidence of cancer, there is an ever-growing need for new cancer treatment strategies. Recently, magnetic nanoparticles have stood out as promising nanostructures for imaging and drug delivery systems as they possess unique properties. Moreover, magnetic nanomaterials functionalized with other compounds can lead to multicomponent nanoparticles with innovative structures and synergetic performance. The incorporation of chemotherapeutic drugs or RNA in magnetic drug delivery systems represents a promising alternative that can increase efficiency and reduce the side effects of anticancer therapy. This review presents a critical overview of the recent literature concerning the advancements in the field of magnetic nanoparticles used in drug delivery, with a focus on their classification, characteristics, synthesis and functionalization methods, limitations, and examples of magnetic drug delivery systems incorporating chemotherapeutics or RNA.

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Fluorescently-tagged magnetic protein nanoparticles for high-resolution optical and ultra-high field magnetic resonance dual-modal cerebral angiography

Abstract: Extremely small paramagnetic iron oxide nanoparticles (FeMNPs) (<5 nm) can enhance positive magnetic resonance imaging (MRI) contrast by shortening longitudinal relaxation time of water (T1), but these nanoparticles experience rapid...

Cited by 5 publications

References 73 publications

Spatiotemporal features of neurovascular (un)coupling with stimulus-induced activity and hypercapnia challenge in cerebral cortex and olfactory bulb

Spatiotemporal features of neurovascular (un)coupling with stimulus-induced activity and hypercapnia challenge in cerebral cortex and olfactory bulb

Comparative transcriptomics revealed neurodevelopmental impairments and ferroptosis induced by extremely small iron oxide nanoparticles

Recent Advances in the Development of Drug Delivery Applications of Magnetic Nanomaterials

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