Fluoride-induced histopathology and synthesis of stress protein in liver and kidney of mice

Abstract

Selective low (15 mg sodium fluoride (NaF)/L) and relatively high (150 mg NaF/L) doses of in vivo fluoride (F) treatment to Swiss albino mice through drinking water elicited organ-specific toxicological response. All the F-exposed groups showed severe alterations in both liver and kidney architectures, but there was no significant change in the rate of water consumption and body weight. Vacuolar degeneration, micronecrotic foci in the hepatocytes, and hepatocellular hypertrophy were evident in the mice exposed to low dose (15 mg NaF/L for 30 days) while sinusoidal dilation with enlarged central vein surrounded by deep-blue erythrocytes were preponderant when treated with the same dose for a period of 90 days. Blood filled spaces, disintegration of tubular epithelium, and atrophy of glomeruli were also recorded in the kidney of the same treatment group. Change in reduced glutathione level (GSH), glutathione-s-transferase (GST) activity, malondialdehyde (MDA) production in both liver and kidney, disturbances in liver function, induction of heat shock protein 70 (Hsp 70) expression in kidney and its down regulation in liver were positively correlated with histopathological lesion.

Differential expression of myocardial heat shock proteins in rats acutely exposed to fluoride

“…*p < 0.05 significantly different from control, + p < 0.05 significantly different from 45 mg F − -treated rats (one-way ANOVA followed by Tukey's multiple comparison test) Earlier studies showed that elevated extracellular levels of Hsp70 serve as a prognostic marker for inflammatory cytokine-mediated immune response and the progression of heart failure in patients with acute AMI (Jenei et al 2013;Satoh et al 2006). Our results are in accordance with the report of Chattopadhyay et al (2011). Their findings showed the increased oxidative stress directly correlated with elevated Hsp70 expression in the mice kidney exposed to chronic F − intoxication.…”

Pesticides induced oxidative stress and histomorphological changes in liver and kidney of female Bandicota bengalensis and Tateraindica

“…Organ weight and Organosomatic index: Following humane sacrifice, the vital organs viz liver and kidney were excised, cleaned off the adhering tissue and weighed separately. The organo-somatic index (OSI) was calculated by using the following formula as per Chattopadhyay et al (2011). Organo -somatic Index (OSI) = Organ weight (g) / Live body weight (g) X 100 Sample preparation : For biochemical studies, 0.5 g of liver and one whole kidney was homogenized in 2 ml of phosphate buffer saline (PBS 0.1M, pH 7.4) and the homogenate was centrifuged at 3000 for r.p.m.…”

Detection of Macro and Micro Nodule Using Online Region Based-Active Contour Model in Histopathological Liver Cirrhosis

“…Chattopadhyay, S. Podder, S. Agarwal, and S. Bhattacharya, [11] have evidently specify the study of Fluoride (F)-induced hepatotoxicity and nephrotoxity in mice by analysing the histopathological changes in the liver, kidney and oxidative stress. To obtain an effective inducer, the Heat Shock Protein (HSP70) in kidney and the liver is negatively regulated in mouse.…”