Aim:The aim of this study was to evaluate and compare the fluoride ion release and uptake of glass ionomer cement GP IX Extra (GE), EQUIA® Forte Fil (EF), Beautifil Bulk (BB), Dyract® XP (DXP), Tetric N-Ceram® (TNC) in vitro.Materials and Methods:The restorative materials were divided into five groups (n = 12), namely G1 – GE, G2 – EF, G3 – BB, G4 – DXP, G5 – TNC. Fluoride release was checked using fluoride ion-selective electrode (Orion 9609BNWP, Ionplus Sure-Flow Fluoride, Thermo Scientific, USA) at time intervals of 1 day and thereafter at 3, 7, 14, 21, and 28 days. Following this, fluoride recharge was done with 1.23% acidulated phosphate fluoride gel (12,300 ppm, Pascal International Inc., 2929 Northup Way, Bellevue, USA) after 28 days and each sample was re-checked for fluoride release at the same time intervals.Statistical Analysis:One-way ANOVA with post hoc Games–Howell test for intergroup and repeated measures ANOVA with post hoc Bonferroni test for intragroup analysis. P < 0.05 was considered statistically significant.Results:On intergroup analysis, Group 2 showed high values of fluoride release and recharge at all-time intervals which were significantly higher (P < 0.001) than Groups 1, 3, 4 and 5. Intragroup analysis showed significantly higher (P < 0.001) fluoride release and recharge at the end of day 1 which decreased progressively with time for all the tested materials.Conclusion:EF showed the highest fluoride release and recharge potential across all time intervals compared to other tested restorative materials and the greatest fluoride release was seen on day 1.