Fluorimetric coupled enzyme assay for lysoplasmalogenase activity in liver

Abstract

We developed a continuous spectrofluorimetric assay of lysoplasmalogenase activity with the use of horse liver alcohol dehydrogenase as a coupling enzyme. In this method the disappearance of NADH is measured spectrofluorimetrically. The excitation and emission monochromators were set at 340 and 460 nm respectively. The assay is 10 times as sensitive as the previous u.v. spectrophotometric method. We could detect approx. 0.02 nmol of aldehyde produced/min per ml.

Purification of plasmalogens usingRhizopus delemar lipase andNaja naja naja phospholipase A2

“…Therefore, the enzymes hydrolyzing plasmalogens may be very important metabolically. Spectrophotometric-and fluorometric-coupled enzyme assay procedures have been developed using auxiliary enzymes (23)(24)(25)(26)(27). Therefore, purified plasmalogens and their metabolites are needed as substrates for plasmalogen hydrolyzing enzymes.…”