Fluorinated diglucose detergents for membrane-protein extraction

Boussambe, Gildas Nyame Mendendy; Guillet, Pierre; Mahler, Florian; Marconnet, Anaïs; Vargas, Carolyn; Cornut, Damien; Soulié, Marine; Ebel, Christine; Roy, Aline Le; Jawhari, Anass; Bonneté, Françoise; Keller, Sandro; Durand, Grégory

doi:10.1016/j.ymeth.2018.05.025

Cited by 20 publications

(46 citation statements)

References 38 publications

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“…The larger micelle size above 5mM, for F 5 DM versus F 5 OM observed from AUC and SAXS is in line with what is generally observed for hydrogenated 42 and fluorinated detergents. 20 Comparing with fluorinated compounds with the same OM head-group, while the hydrogenated DDM forms small slightly elongated micelles of 60 kDa up to at least 10 mM 29 , the poorly fluorinated F 2 UDM and the nonyl derivative with four perfluorinated carbons F 4 NM (also called F 4 H 5 Malt) 22 were described to form small micelles with N agg < 100 up to C- shown in Figure 4 support the interpretation that the decreased light scattering intensity was due to vesicle solubilization, as the vesicular peak at ∼120 nm at the beginning of the measurement completely disappeared after the intensity decreased to the level of pure mixed micelles. Solubilization was essentially complete after ∼16 h for F 5 DM but took longer for FhuA is an E. coli outer membrane ferrichrome-iron transporter involved in bacteriophage infection.…”

Section: Resultsmentioning

confidence: 99%

“…The commercial F 6 OM forms very large rod micelles with N agg > 500 at CMC + 10 mM (s of 27 S at CMC + 5 mM). The propensity of highly fluorinated surfactants with a OM head to form rather large rods is in contrast with fluorinated surfactants with a head bearing two glucose groups, which self-assemble into compact and well-defined globular micelles of 6-8 nm in diameter with aggregation numbers below 100 20. Solubilization of POPC LUVs by FSs.…”

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confidence: 99%

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Maltose-Based Fluorinated Surfactants for Membrane-Protein Extraction and Stabilization

et al. 2021

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Two new surfactants, F 5 OM and F 5 DM, were designed as partially fluorinated analogs of ndodecyl-β-D-maltoside (DDM). The micellization properties and the morphologies of the aggregates formed by the two surfactants in water and phosphate buffer were evaluated by NMR spectroscopy, surface tension measurement (SFT), isothermal titration calorimetry (ITC), dynamic light scattering (DLS), small-angle X-ray scattering (SAXS), and analytical ultracentrifugation (AUC). As expected, the critical micellar concentration (CMC) was found to decrease with chain length of the fluorinated tail from 2.1-2.5 mM for F 5 OM to 0.3-0.5 mM for F 5 DM, and micellization was mainly entropy-driven at 25°C. Close to their respective CMC, the micelle sizes were similar for both surfactants i.e. 7 and 13 nm for F 5 OM and F 5 DM, respectively and both increased with concentration forming 4 nm diameter rods with maximum dimensions of 50 and 70 nm, respectively, at a surfactant concentration of ~30 mM. The surfactants were found to readily solubilize lipid vesicles and extract membrane proteins (MPs) directly from Escherichia coli membranes. They were found more efficient than the commercial fluorinated detergent F 6 H 2 OM over a broad range of concentrations (1-10 mM) and even better than DDM at low concentrations (1-5 mM). When transferred into the two new surfactants, the thermal stability of the proteins bacteriorhodopsin (bR) and FhuA were higher than in the presence of their solubilization detergents and similar to that in DDM; furthermore, bR was stable over several months. The membrane enzymes SpNOX and BmrA were not as active as in DDM micelles but similarly active as in F 6 OM. Together, these findings indicate both extracting and stabilizing properties of the new maltose-based fluorinated surfactants, making them promising tools in MPs applications.

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Section: Resultsmentioning

confidence: 99%

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Maltose-Based Fluorinated Surfactants for Membrane-Protein Extraction and Stabilization

et al. 2021

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“…As mentioned before, in a series of publications, Durand and coworkers, used Equation (18) to analyse 19 F NMR-shift data and obtained good coincidence in the cmc values obtained with several other techniques [ 41 , 42 , 43 ].…”

Section: Application To Different Techniquesmentioning

confidence: 89%

A Surfactant Concentration Model for the Systematic Determination of the Critical Micellar Concentration and the Transition Width

Al‐Soufi

Novo

2021

Molecules

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The critical micellar concentration (cmc) is a fundamental property of surfactant solutions. Many proposed methods for the definition and determination of the cmc from property-concentration plots yield values, which depend on the studied property, on the specific technique used for its analysis and in many cases on the subjective choice of the chosen type of plot and concentration interval. In this focus review, we revise the application of a surfactant concentration model we proposed earlier that defines the cmc directly based on the surfactant concentration. Known equations for the concentration-dependence of different surfactant properties can then be combined with this concentration model and fitted to experimental data. This modular concept makes it possible to determine the cmc and the transition width in a systematic and unambiguous way. We revise its use in the literature in different contexts: the determination of the cmc of surfactants and their mixtures from different properties (electrical conductivity, NMR chemical shift, self-diffusion, surface tension, UV-Vis absorption, fluorescence intensity and fluorescence correlation). We also revise the dependence of the width of the transition region on composition, detailed studies of the properties of fluorescent probes and the aggregation of non-surfactant systems, namely amyloid peptides.

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“…A range of surfactants and polymers such as fluorinated surfactants 23 , styrene--maleic acid copolymers 24 and amphiphipols 25 are being explored for improvements over traditional detergents. The development of surfactants able to extract membrane proteins from their lipid environment more efficiently and better maintain their stability will greatly aid the study of these proteins.…”

Section: Detergentsmentioning

confidence: 99%

Liposomes as models for membrane integrity

Routledge

Linney

Goddard

2019

Biochemical Society Transactions

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Biological membranes form the boundaries to cells. They are integral to cellular function, retaining the valuable components inside and preventing access of unwanted molecules. Many different classes of molecules demonstrate disruptive properties to the plasma membrane. These include alcohols, detergents and antimicrobial agents. Understanding this disruption and the mechanisms by which it can be mitigated is vital for improved therapeutics as well as enhanced industrial processes where the compounds produced can be toxic to the membrane. This mini-review describes the most common molecules that disrupt cell membranes along with a range of in vitro liposome-based techniques that can be used to monitor and delineate these disruptive processes.

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Fluorinated diglucose detergents for membrane-protein extraction

Cited by 20 publications

References 38 publications

Maltose-Based Fluorinated Surfactants for Membrane-Protein Extraction and Stabilization

Maltose-Based Fluorinated Surfactants for Membrane-Protein Extraction and Stabilization

A Surfactant Concentration Model for the Systematic Determination of the Critical Micellar Concentration and the Transition Width

Liposomes as models for membrane integrity

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