Fluorinated PLGA-PEG-Mannose Nanoparticles for Tumor-Associated Macrophage Detection by Optical Imaging and MRI

Zambito, Giorgia; Deng, Siyuan; Haeck, Joost; Gaspar, Natasa; Himmelreich, Uwe; Censi, Roberta; Löwik, Clemens; Martino, Piera Di; Mezzanotte, Laura

doi:10.3389/fmed.2021.712367

Cited by 12 publications

(4 citation statements)

References 31 publications

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“…The peak observed at 3.64 ppm corresponds to the methylene group in the PEG segment. The peak of mannosamine in figure 1(e) overlapped with the peak of PEG (3.62 ppm), so only a small peak at about 4.2-4.3 ppm was detected, which was attributed to mannosamine [33,34]. Since mannose was only modified at one end of this polymer material, the peak of mannosamine was low, which was also verified in the work of Zhu et al [23].…”

Section: Characterization Of Plga-peg-mansupporting

confidence: 71%

Immune cell receptor-specific nanoparticles as a potent adjuvant for nasal split influenza vaccine delivery

Li,

Xiu,

et al. 2024

Nanotechnology

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Mucosal delivery systems have gained much attention as effective way for antigen delivery that induces both systemic and mucosal immunity. However, mucosal vaccination faces the challenges of mucus barrier and effective antigen uptake and presentation. In particular, split, subunit and recombinant protein vaccines that do not have an intact pathogen structure lack the efficiency to stimulate mucosal immunity. In this study, poly (lactic acid-co-glycolic acid-polyethylene glycol) (PLGA-PEG) block copolymers were modified by mannose to form a PLGA-PEG-Man conjugate (mannose modified PLGA-PEG), which were characterized. The novel nanoparticles (NPs) prepared with this material had a particle size of about 150 nm and a zeta potential of -15 mV, and possessed ideal mucus permeability, immune cell targeting, stability and low toxicity. Finally, PLGA-PEG-Man nanoparticles (PLGA-PEG-Man NPs) were successfully applied for intranasal delivery of split influenza vaccine in rat for the first time, which triggered strong systemic and mucosal immune responses. These studies suggest that PLGA-PEG-Man NPs could function as competitive potential nano-adjuvants to address the challenge of inefficient mucosal delivery of non-allopathogenic antigens.

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Section: Characterization Of Plga-peg-mansupporting

confidence: 71%

Immune cell receptor-specific nanoparticles as a potent adjuvant for nasal split influenza vaccine delivery

Li,

Xiu,

et al. 2024

Nanotechnology

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“…The cells were fixed with 4% paraformaldehyde (PA) solution after incubation and stained with DAPI (Sigma, Saint Louis, United States). A confocal microscope was used to observe the treated cells ( Zambito et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

β-Cyclodextrin and Oligoarginine Peptide-Based Dendrimer-Entrapped Gold Nanoparticles for Improving Drug Delivery to the Inner Ear

Luo

Lin

et al. 2022

Front. Bioeng. Biotechnol.

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Here, we developed a safe and highly effective nanocarrier using β-cyclodextrin (β-CD) and oligoarginine peptide (Arg8)-modified dendrimer-entrapped gold nanoparticles (Au@CD-PAMAM-Arg8), with a diameter of 5 nm, for improved delivery of dexamethasone (Dex) to the inner ear. The properties and in vivo distribution of the Au@CD-PAMAM-Arg8 were assessed in vitro, and a streptomycin (SM) ototoxicity model was used in vivo. Flow cytometry analysis of HEIOC1 cells treated with Au@CD-PAMAM-Arg8 and Au @CD-PAMAM at different time intervals indicated that cell uptake efficiency of the drug delivery carrier Au@CD-PAMAM-Arg8 was higher than that of Au @CD-PAMAM. Au@CD-PAMAM-Arg8 carrying Dex (Au@CD-PAMAM-Arg8/Dex) were mainly distributed in hair cells, the spiral ganglion, lateral wall, and nerve fibers and had stronger protective effects on the inner ear than Dex administration alone. In vivo tracer tests revealed that tympanic injection was significantly more effective than posterior ear injection, muscle injection, and tail vein injection, whereas clinical retro-auricular injection could not increase the efficiency of drug delivery into the ear. Electrocochleography results showed that Au@CD-PAMAM-Arg8/Dex significantly improved hearing in C57/BL6 mice after SM exposure. These findings indicate that Au@CD-PAMAM-Arg8 may be the useful drug carriers for the treatment of inner ear diseases.

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“…Tumour-associated macrophages (TAM) are tumour-promoting inflammations that could be potential biomarkers for diagnosis, prognosis, and therapeutic targets for cancer [213]. Zambito et al used PLGA-PEG-mannose NPs encapsulated with PFCE to visualize TAMs by optical imaging and 19 F MRI [214] with higher specificity and robust signal strength. NIR dye encapsulated PLGA-PEG NPs were adapted for monitoring and imaging in osteoarthritis by modifying the NPs with trifluoroacetamide [215].…”

Section: Polymeric Npsmentioning

confidence: 99%

Nanotechnology as a Versatile Tool for 19F-MRI Agent’s Formulation: A Glimpse into the Use of Perfluorinated and Fluorinated Compounds in Nanoparticles

et al. 2022

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Simultaneously being a non-radiative and non-invasive technique makes magnetic resonance imaging (MRI) one of the highly sought imaging techniques for the early diagnosis and treatment of diseases. Despite more than four decades of research on finding a suitable imaging agent from fluorine for clinical applications, it still lingers as a challenge to get the regulatory approval compared to its hydrogen counterpart. The pertinent hurdle is the simultaneous intrinsic hydrophobicity and lipophobicity of fluorine and its derivatives that make them insoluble in any liquids, strongly limiting their application in areas such as targeted delivery. A blossoming technique to circumvent the unfavorable physicochemical characteristics of perfluorocarbon compounds (PFCs) and guarantee a high local concentration of fluorine in the desired body part is to encapsulate them in nanosystems. In this review, we will be emphasizing different types of nanocarrier systems studied to encapsulate various PFCs and fluorinated compounds, headway to be applied as a contrast agent (CA) in fluorine-19 MRI (19F MRI). We would also scrutinize, especially from studies over the last decade, the different types of PFCs and their specific applications and limitations concerning the nanoparticle (NP) system used to encapsulate them. A critical evaluation for future opportunities would be speculated.

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Fluorinated PLGA-PEG-Mannose Nanoparticles for Tumor-Associated Macrophage Detection by Optical Imaging and MRI

Cited by 12 publications

References 31 publications

Immune cell receptor-specific nanoparticles as a potent adjuvant for nasal split influenza vaccine delivery

Immune cell receptor-specific nanoparticles as a potent adjuvant for nasal split influenza vaccine delivery

β-Cyclodextrin and Oligoarginine Peptide-Based Dendrimer-Entrapped Gold Nanoparticles for Improving Drug Delivery to the Inner Ear

Nanotechnology as a Versatile Tool for 19F-MRI Agent’s Formulation: A Glimpse into the Use of Perfluorinated and Fluorinated Compounds in Nanoparticles

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