Fluorine analysis of human enamel around fluoride-containing materials under different pH-cycling by μ-PIGE/PIXE system

Komatsu, Hirokazu; Yamamoto, Hiroko; Matsuda, Yasuhiro; Kijimura, T.; Kinugawa, M.; Okuyama, Katsushi; Nomachi, M.; Yasuda, Keisuke; Satoh, Takahiro; Oikawa, S.

doi:10.1016/j.nimb.2011.02.042

Cited by 21 publications

(33 citation statements)

References 8 publications

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“…The assessment site was a 500×270 µm area of enamel around the fissure sealant. The settings for the device were determined in accordance with the methods described by Komatsu et al 23,24) . The specimens were bombarded with a 1.7-MeV proton beam with a diameter of ~1 µm and a current of ~100 pA.…”

Section: Assessment Of Fluoride Levels In Enamelmentioning

confidence: 99%

Assessment of the inhibitory effects of fissure sealants on the demineralization of primary teeth using an automatic pH-cycling system

et al. 2016

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In this study, we examined the effects of fissure sealants on inhibition of demineralization of primary teeth using an automatic pHcycling system. Three fissure sealants were used: Teethmate F-1 2.0 (TM), BeautiSealant (BS), and Fuji III LC (IIILC). Using an automatic pH-cycling system, the specimens (n=12) were repeatedly demineralized and remineralized. Specimens were subjected to transverse microradiography (TMR), and changes in integrated mineral loss (IML) and lesion depth (Ld), indicated as ΔIML and ΔLd, respectively, were calculated. In addition, fluoride levels in the enamel were assessed using microparticle-induced gammaray emission/particle-induced X-ray emission (n=3). IIILC showed the lowest values for ΔIML and ΔLd, followed by BS and then TM. The highest amount of fluorine in the enamel was observed for IIILC, followed by TM and BS. All fissure sealants inhibited demineralization in primary teeth.

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Section: Assessment Of Fluoride Levels In Enamelmentioning

confidence: 99%

Assessment of the inhibitory effects of fissure sealants on the demineralization of primary teeth using an automatic pH-cycling system

et al. 2016

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“…Micro-PIGE/PIXE analysis was performed as previously described 20,21) . In brief, a 3.0-MeV proton beam was emitted from an ion microbeam apparatus ( Fig.…”

Section: Micro Pige/pixe Analysismentioning

confidence: 99%

“…Ca and Sr concentrations were measured by micro-PIXE, which was simultaneously performed with a silicon lithium detector in vacuum 38) . The beam intensity was monitored according to the X-ray yield from a copper (Cu) foil for quantitative analysis 21) . Quantitative analysis of trace elements in PIGE/ PIXE is performed based on the counts of characteristic gamma/X-rays discharged from specimens.…”

Section: Micro Pige/pixe Analysismentioning

confidence: 99%

“…Several restorative materials such as conventional type glass ionomer cements or resin composites containing fluoride and Sr (FSCMs) are assumed to enable uptake of fluorine (F) and Sr [17][18][19] by dental hard tissue. These elements contained in filled FSCMs are incorporated into enamel as precipitation of eluted elements into enamel surface and/or direct uptake by enamel cavity wall, and affect the de-and remineralization of the enamel 20,21) . To evaluate uptake and the anticarious effects of these elements, their distributions in enamel must be analyzed.…”

Section: Introductionmentioning

confidence: 99%

“…PIXE detects characteristic X-rays from elements with more than 2 electron shells, whereas relatively light elements such as F are detected with PIGE 33,34) . In previous studies, the distribution of F around fluoride-containing materials was mapped and F uptake by human enamel was analyzed by PIGE/PIXE 20,21) , which could provide quantitative distribution data at the µm level (micro PIGE/PIXE system) in the air. In this in vitro study, we aimed to establish a technique to analyze the Sr and F uptake by human enamel using the micro PIGE/PIXE system.…”

Section: Introductionmentioning

confidence: 99%

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A new technique for analyzing trace element uptake by human enamel

et al. 2015

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Fluorine (F) and strontium (Sr) are key elements in the de-and remineralization of teeth. To quantitatively analyze the distribution of F and Sr, micro-particle-induced gamma/X-ray emission (PIGE/PIXE) technique was used. The cavities were prepared and filled with the fluoride-and Sr-containing restorative materials (FSCMs) in extracted human molars. The single-section enamel specimens were prepared by slicing from the buccal to lingual surface including the FSCMs. After 5 weeks of automatic pH cycling, the demineralization was calculated by integrated mineral loss (ΔIML) from transverse-microradiography. The distributions of F and Sr were analyzed by the PIGE/PIXE technique. The micro-PIGE/PIXE technique indicated a fluorine uptake difference between the enamel surface and enamel cavity wall. ΔIML of FSCMs were significantly lower than intact enamel. The micro-PIGE/PIXE technique enables measurement of F and Sr uptake from FSCMs into enamel, which would be beneficial for research on caries development and prevention.

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Evaluation of enamel demineralization and fluorine uptake caused by gustatory stimulants of salivary secretion (GSSS) using Raman spectroscopy and proton induced gamma‐ray emission (PIGE)

Luis

Silva

Silveira

et al. 2018

J Raman Spectroscopy

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In this work, two gustatory stimulants of salivary secretion (GSSS) were studied using Raman microscopy to evaluate their potential demineralization effect in human enamel samples and proton induced gamma-ray emission (PIGE) to quantify fluorine uptake. These pharmaceutical products are usually administrated to patients who have revealed xerostomia, namely, caused by radiation therapy, chemotherapy, or diseases such as Sjögren's Syndrome. Traditional GSSS are composed of citric or malic acids that can promote enamel erosion by decreasing salivary pH below the critical level of hydroxyapatite demineralization (pH = 5.5). Recently, new GSSS composed of malic acid, fluorine, and xylitol were developed, which are supposed to decrease erosion potential risk and uptake fluorine in dental enamel. In this, in vitro study, 16 specimens of human enamel were randomized in two different groups exposed to two different GSSS: S group-SST® (with citric and malic acids, without fluorine) and X group-Xeros™ (with malic acid, fluorine, and xylitol) and analysed before and after treatment. The treatment simulated administration of four tablets per day during eight consecutive days. Statistically, significant increase in fluorine concentration was found in samples treated by Xeros™, whereas no significant differences were found in fluorine concentration for the ones treated using SST® treatment. No demineralization was found in dental enamel samples treated with either Xeros™ or SST®.

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Fluorine analysis of human enamel around fluoride-containing materials under different pH-cycling by μ-PIGE/PIXE system

Cited by 21 publications

References 8 publications

Assessment of the inhibitory effects of fissure sealants on the demineralization of primary teeth using an automatic pH-cycling system

Assessment of the inhibitory effects of fissure sealants on the demineralization of primary teeth using an automatic pH-cycling system

A new technique for analyzing trace element uptake by human enamel

Evaluation of enamel demineralization and fluorine uptake caused by gustatory stimulants of salivary secretion (GSSS) using Raman spectroscopy and proton induced gamma‐ray emission (PIGE)

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