Fluorogenic Detection of Human Serum Albumin Using Curcumin-Capped Mesoporous Silica Nanoparticles

Otri, Ismael; Medaglia, Serena; Aznar, Elena; Sancenón, Félix; Martínez‐Máñez, Ramón

doi:10.3390/molecules27031133

Cited by 6 publications

(4 citation statements)

References 43 publications

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“…To obtain the S1 nanomaterial, ordered mesoporous nanoparticles were prepared through known procedures using n-cetyltrimethylammonium bromide (CTABr) as a template and tetraethylorthosilicate (TEOS) as a silicon precursor [ 37 ]. After removing the surfactant through calcination, the empty pores were loaded with linezolid and the external surface was functionalized using n-[(3-trimethoxysilyl)propyl]ethylene diamine triacetic acid trisodium salt to obtain a negatively charged surface.…”

Section: Resultsmentioning

confidence: 99%

Exploring the Synergy between Nano-Formulated Linezolid and Polymyxin B as a Gram-Negative Effective Antibiotic Delivery System Based on Mesoporous Silica Nanoparticles

Otri,

Medaglia,

Martínez-Máñez

et al. 2024

Nanomaterials

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Antimicrobial resistance is a current silent pandemic that needs new types of antimicrobial agents different from the classic antibiotics that are known to lose efficiency over time. Encapsulation of antibiotics inside nano-delivery systems could be a promising, effective strategy that is able to delay the capability of pathogens to develop resistance mechanisms against antimicrobials. These systems can be adapted to deliver already discovered antibiotics to specific infection sites in a more successful way. Herein, mesoporous silica nanomaterials are used for an efficient delivery of a linezolid gram-positive antibiotic that acts synergistically with gram-negative antimicrobial polymyxin B. For this purpose, linezolid is encapsulated in the pores of the mesoporous silica, whose outer surface is coated with a polymyxin B membrane disruptor. The nanomaterial showed a good controlled-release performance in the presence of lipopolysaccharide, found in bacteria cell membranes, and the complete bacteria E. coli DH5α. The performed studies demonstrate that when the novel formulation is near bacteria, polymyxin B interacts with the cell membrane, thereby promoting its permeation. After this step, linezolid can easily penetrate the bacteria and act with efficacy to kill the microorganism. The nano-delivery system presents a highly increased antimicrobial efficacy against gram-negative bacteria, where the use of free linezolid is not effective, with a fractional inhibitory concentration index of 0.0063 for E. coli. Moreover, enhanced toxicity against gram-positive bacteria was confirmed thanks to the combination of both antibiotics in the same nanoparticles. Although this new nanomaterial should be further studied to reach clinical practice, the obtained results pave the way to the development of new nanoformulations which could help in the fight against bacterial infections.

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Section: Resultsmentioning

confidence: 99%

Exploring the Synergy between Nano-Formulated Linezolid and Polymyxin B as a Gram-Negative Effective Antibiotic Delivery System Based on Mesoporous Silica Nanoparticles

Otri,

Medaglia,

Martínez-Máñez

et al. 2024

Nanomaterials

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“…The solvent was a 1:9 mixture of ACN and distilled water solution [ 18 ] and was used in every experiment as a solvent. The synthesis of 3-hydroxymethyl-1-oxyl-4-(pyren-1-yl)-2,2,5,5-tetramethyl-2,5-dihydro-1 H -pyrrole radical and tetrakis(3,5-dicarboxylatophenoxy)-cavitand was carried out in our institute according to published methods [ 12 , 13 ], and their 1 H NMR scan was taken.…”

Section: Methodsmentioning

confidence: 99%

A New Application of Spin and Fluorescence Double-Sensor Molecules

et al. 2023

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EPR imaging techniques are known to be successful tools for mapping living bodies, especially because of the high transparency of tissues in the microwave range. This technique assumes the presence of radicals whose in vivo transport is also controlled by serum albumins. Accordingly, in this study, the interactions between 3-hydroxymethyl-1-oxyl-4-(pyren-1-yl)-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrole radical and the human serum albumin molecules were investigated. To clarify the adsorption processes of this radical onto the surface of human serum albumin (HSA), the interaction of the OMe derivative of the radical was also examined parallel with the studies on the radical—HSA interactions. Considering the solubility issues and also to modulate the transport, inclusion complexes of the radical with a cavitand derivative were also studied. The latter interactions were observed through fluorescence spectroscopy, fluorescence polarization, and by EPR spectroscopy. As a double-sensor molecule, we found that the fluorophore nitroxide is a good candidate as it gave further information about host-guest interactions (fluorescence, fluorescence polarization, and EPR). We also found that in the presence of a cavitand, a complex with greater stability was formed between the sensor molecule and the human serum albumin. Based on these observations, we can conclude that applying this double-sensor (spin, fluorescent) molecule is useful in cases when different interactions can affect the EPR measurements.

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“…Rhodamine B is selectively released from nanoparticles in the presence of albumin due to the opening of pores (uncapping of the nanoparticles) caused by the formation of a complex between albumin and Cur. 93 …”

Section: Synthesis Of Cur Loaded-msnsmentioning

confidence: 99%

Curcumin-loaded mesoporous silica nanoparticles for drug delivery: synthesis, biological assays and therapeutic potential – a review

Iranshahy,

Hanafi-Bojd,

Aghili

et al. 2023

RSC Adv.

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Fluorogenic Detection of Human Serum Albumin Using Curcumin-Capped Mesoporous Silica Nanoparticles

Cited by 6 publications

References 43 publications

Exploring the Synergy between Nano-Formulated Linezolid and Polymyxin B as a Gram-Negative Effective Antibiotic Delivery System Based on Mesoporous Silica Nanoparticles

Exploring the Synergy between Nano-Formulated Linezolid and Polymyxin B as a Gram-Negative Effective Antibiotic Delivery System Based on Mesoporous Silica Nanoparticles

A New Application of Spin and Fluorescence Double-Sensor Molecules

Curcumin-loaded mesoporous silica nanoparticles for drug delivery: synthesis, biological assays and therapeutic potential – a review

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