Fluorogenic probes for super-resolution microscopy

Kozma, Eszter; Kele, Péter

doi:10.1039/c8ob02711k

Cited by 114 publications

(95 citation statements)

References 121 publications

(102 reference statements)

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“…The uorogenic FL Tz probe is also useful for super resolution microscopy (SRM) imaging. 79,[87][88][89][90] In 2018 the Kele group reported uorogenic cyanine-tetrazine conjugates for no-wash subsequent super-resolution microscopy (STED) imaging of an actin lament. To quench the cyanine dye via the TBET strategy along with bioorthogonality, they designed 4 different tetrazinecyanine dyes utilizing either a phenylene or a vinylene linker between tetrazine and the uorophore.…”

Section: Tetrazine-based Turn-on Probementioning

confidence: 99%

Biomedical applications of copper-free click chemistry: in vitro, in vivo, and ex vivo

2019

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Section: Tetrazine-based Turn-on Probementioning

confidence: 99%

Biomedical applications of copper-free click chemistry: in vitro, in vivo, and ex vivo

2019

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“…(B) Combining super-resolution techniques. Super-resolution techniques, such as stimulated emission depletion (STED), structured illumination microscopy (SIM) and stochastic optical reconstruction microscopy (STORM), provide a valuable starting point to study analytes in nano-dimension (Kozma and Kele 2018). Application of this technique to molecular probes for mapping metals is an important and arduous task.…”

Section: Summary and Perspectivesmentioning

confidence: 99%

Recent Endeavors on Molecular Imaging for Mapping Metals in Biology

et al. 2020

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Transition metals such as zinc, copper and iron play vital roles in maintaining physiological functions and homeostasis of living systems. Molecular imaging, including two-photon imaging (TPI), bioluminescence imaging (BLI) and photoacoustic imaging (PAI), could act as non-invasive toolkits for capturing dynamic events in living cells, tissues and whole animals. Herein, we review the recent progress in the development of molecular probes for essential transition metals and their biological applications. We emphasize the contributions of metallostasis to health and disease, and discuss the future research directions about how to harness the great potential of metal sensors. Graphic Abstract

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“…fluorogens such as SiR-tubulin and SiR-actin) ( Fig. 4b, left) (80,81) or cell compartments directly (e.g. Membright, ER-Tracker or MitoTracker) ( Fig.…”

Section: Fluorescent Probe Development For Live-cell Super-resolutionmentioning

confidence: 99%

Between Life and Death: Reducing Phototoxicity in Super-Resolution Microscopy

Tosheva¹,

Yuan²,

Pereira³

et al. 2019

Preprint

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Super-Resolution Microscopy enables non-invasive, moleculespecific imaging of the internal structure and dynamics of cells with sub-diffraction limit spatial resolution. One of its major limitations is the requirement for high-intensity illumination, generating considerable cellular phototoxicity. This factor considerably limits the capacity for live-cell observations, particularly for extended periods of time. Here, we overview new developments in hardware, software and probe chemistry aiming to reduce phototoxicity. Additionally, we discuss how the choice of biological model and sample environment impacts the capacity for live-cell observations. Phototoxicity | Photodamage | Super-Resolution Microscopy | Fluorescence Correspondence: s.culley@ucl.ac.uk, r.henriques@ucl.ac.uk Tosheva et al. | 1-12Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted:

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Fluorogenic probes for super-resolution microscopy

Abstract: Fluorogenic probes efficiently reduce non-specific background signals, which often results in highly improved signal-to-noise ratios.

Cited by 114 publications

References 121 publications

Biomedical applications of copper-free click chemistry: in vitro, in vivo, and ex vivo

Biomedical applications of copper-free click chemistry: in vitro, in vivo, and ex vivo

Recent Endeavors on Molecular Imaging for Mapping Metals in Biology

Between Life and Death: Reducing Phototoxicity in Super-Resolution Microscopy

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