Fluorographic detection of radioactivity in polyacrylamide gols with the water-soluble fluor, sodium salicylate

Chamberlain, John P.

doi:10.1016/0003-2697(79)90716-4

Cited by 1,993 publications

(640 citation statements)

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“…In later experiments the method described by Chamberlain (1979) was used, in which gels were soaked in water and then in 1M sodium salicylate for 30min each.…”

Section: Autoradiographl and Fluorograehymentioning

confidence: 99%

In vitro synthesis of barley storage proteins

Matthews

Miflin

1980

Planta

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SummaryMembrane-bound polysomes were isolated from developing endosperms of barley (Hordeum vulgare L.). The me~senger RNA associated with the polysomes was separated from the ribosomal components by affinity chromatography on oligo-dT cellulose. The characteristics of t~e polysomes and of the poly-A+ RNA fraction are discussed. The poly-AT RNA fraction contained components of between 0.55 and 2.5 kilobases in length, and the degree of adenylation is 6.5%. Both polysomes and poly-A~

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“…In later experiments the method described by Chamberlain (1979) was used, in which gels were soaked in water and then in 1M sodium salicylate for 30min each.…”

Section: Autoradiographl and Fluorograehymentioning

confidence: 99%

In vitro synthesis of barley storage proteins

Matthews

Miflin

1980

Planta

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“…The reaction mixture was chromatographed on a Biogel P-6 column to remove unincorporated [35Slmethionine. The eluate was loaded on a tube gel and resolved by PP-PAGE as described above, transferred to nitrocellulose, stained with AB [10] and prepared for fluorography [12].…”

Section: Methodsmentioning

confidence: 99%

“…After 45 rain of equilibration, MHC and MLC were separated on a 5-18070 linear gradient or 15070 slab gel by SDS-PAGE [13]. To compare the actual subunit composition with the radiolabeled subunits, the gels were either silver stained [14] or prepared for fluorography as previously described [12].…”

Section: Methodsmentioning

confidence: 99%

Synthesis and assembly of native myosin on muscle polyribosomes

1989

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We have used the overload-induced growth of avian muscles to study the assembly of the newly synthetized myosins which were separated by non-denaturing pyrophosphate-polyacrylamide gel electrophoresis. Using this model, we have observed the appearance of fast-like isomyosins in polyribosomes prepared from slow anterior latissimus dorsi muscle after 72 h of overload. These new isoforms comigrating with native myosifi from fast posterior latissimus dorsi musclewere not yet present in cellular extracts from the same muscle. The in vitro translation system utilizing muscle specific polyribosomes directs the synthesis of the corresponding myosin isoforms. Under denaturing conditions, myosin heavy chains and light chains dissociate to the expected subunit composition of each specific isoform. The synthesis and assembly of native myosin on polyribosomes indicate that myosin exists as a single mature protein prior to the incorporation in the thick filament.Myosin isoform; Myosin heavy chain; Myosin light chain; Overload hypertrophy; (Chicken skeletal muscle)

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“…Electrophoresis was done on 12.5% polyacrylamide gel slabs containing SDS, using separation systems either lacking [9] or containing [2,4] urea. After electrophoresis, the gels were soaked for 30 min in 7% acetic acid and impregnated with sodium salicylate [10] for fluorographic analysis. Protein was measured by the biuret method.…”

Section: Methodsmentioning

confidence: 99%