Fluorometric Analysis

Abstract

INA review of fluorescent analysis as applied to inorganic material appearing in this journal in 1939 (117), several books devoted to this subject were mentioned. Some of these have been revised and serve as excellent references. Danckwortt's book (20) which is specifically concerned with analysis in ultraviolet light, was revised in 1940 and has appeared in this country in lithoprinted form. This contains 1583 references.Feigl (29) gives many fluorometric applications for organic as well as inorganic compounds. Radley and Grant (92) also have an enlarged third edition; Chapter 8 which is on inorganic analysis has 123 references. Texts dealing with colorimetric analysis almost invariably give a discussion of fluorometric techniques. Sandell (100) gives methods for several elements and the Snells (107) in Chapter 15 of their third edition cover some general principles and apparatus for fluorescent analysis. Because of the listing of thq fluorescent color of many substancesfor example, 2896 organic compounds-the analyst will be interested in DeMent's book ( 22); how'ever, the review' by Fonda (33) should be read in connection with this. From a theoretical standpoint the Cornell symposium papers on solid luminescent materials edited by Fonda and Seitz (84), and the paper by Kasha (55) are of interest.

Improved Photoelectric Fluorometer

“…They were used first as simple qualitative reagents for various inorganic and biological materials, but in recent years many precise quantitative methods have been developed for a wide variety of substances. No attempt is made here to review this field, as two comprehensive reviews of fluorometric analysis have been published recently (7,8).…”

“…Visual comparison methods have been used (3), and various, more or less successful, attempts have been made to adapt spectrophotometers to fluorometric measurements (4). At present, there are at least ten or twelve instruments on the market which have been designed for photofluorometric work, and several others have been described (7,8). Practical experience has shown, however, that most of these suffer from one or more of the following objections: lack of stability, inadequate sensitivity, insufficient flexibility' to meet widely varying conditions, or excessive cost.…”