Fluorometric detection and determination of organic compounds. II. Identification of aldehyde derivatives by chromatography

Fluorometric assay of .alpha.-methylene carbonyl compounds with N1-methylnicotinamide chloride

“…The ability of the Hamilton hydroxylation system (1-3), consisting of catechol, ferric perchlorate, and hydrogen peroxide, to introduce a hydroxy group on an aromatic ring is potentially useful analytically. This hydroxylation system was previously used (4,5) as a reagent for the analysis of aqueous solutions of aromatic compounds by their conversion to phenols, which were subsequently determined colorimetrically. The purpose of the present research was to carry out the hydroxylation step and then to measure the resulting hydroxylated product fluorimetrically, because many hydroxy compounds fluoresce strongly.…”

Determination of carbonyl compounds by 2-diphenylacetyl-1,3-indandione-1-hydrazone

“…<* Shape of zone after chromatographic process. 6 First number refers to distance from origin to the end of the tail while second number refers to distance solvent front has traveled.…”

“…Increasing the concentration of ethyl alcohol, benzene, or ethyl acetate when mixed with hexane has the same effect as increasing the concentration of CHC13. If methyl alcohol: H20 (8:1), which was used as eluting agent in qualitative studies on paper (6), is used, tailing for the azine derivative is observed. As the water content is increased, movement of both the azine derivative and hydrazone reagent is reduced.…”

Chromatography

“…PC of oxo compound has been reviewed by Macek (1213). The 2,4-dinitrophenylhydrazones are most commonly used for PC (535, I864, 2024, %1%5), but azine derivatives (205,206) have also been suggested. PC of anthraquinone derivatives (438,1187), particularly of aloin (180) and benzoquinones (1543), has been reported.…”