Fluorometric Determination of Chlorophyll

Holm‐Hansen, Osmund; Lorenzen, Carl J.; Holmes, Robert W.; Strickland, John

doi:10.1093/icesjms/30.1.3

Cited by 2,225 publications

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“…Determinations of POC and PON were performed on filters with a Perkin-Elmer CHN 2400 analyser. Concentrations of chl a were measured by a fluorimetric method (Holm-Hansen et al 1965) after filtration of 500 ml through a Whatman GF/F filter. Methods are detailed in Souchu et al (1997).…”

Section: Methodsmentioning

confidence: 99%

Influence of shellfish farming activities on the biogeochemical composition of the water column in Thau lagoon

Souchu

Vaquer²,

Collos³

et al. 2001

Mar. Ecol. Prog. Ser.

148

108

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Thau lagoon is a Mediterranean shellfish ecosystem with large biomasses of oysters growing in waters with high residence time due to low tidal ranges. The influence of filter feeders (oysters and their epibiota) on the spatial distribution of particulate and dissolved compounds in the water column of Thau lagoon was studied through its variation with time. In 1991/1992, daily variations were investigated in pens, corridors and outside shellfish farming zones for nutrients, chlorophyll a and primary production. Salinity, dissolved oxygen, nutrients, organic matter and chlorophyll a were also monitored in surface waters inside and outside shellfish farming zones each week from January 1993 to March 1994. The presence of shellfish farms led to a decrease by only a few percent of oxygen concentrations in their vicinity, but the mean (± SE) deficits of chlorophyll a and POC concentrations were 44 ± 4% and 26 ± 9% respectively in the eastern zone (8 m). The shift induced by filter feeders in phytoplankton composition favoured picophytoplankton with higher growth rates. But the summer increase in phytoplankton growth rate was stronger than the positive feedback due to filter feeder filtration. Summer was determinant for the growth of oysters owing to enhanced regenerated primary production. During this period, filter feeders were not food limited, while they tended to control phytoplankton biomasses and production the rest of the year. The nutrient excess in shellfish farming zones was highly significant, with increases of 73 ± 16, 36 ± 12 and 19 ± 8% for ammonia, phosphates and silicate respectively in the eastern zone. In the western zone, the nutrient excess was less strong by half for ammonia and phosphate, because the lower depth (4 m) allows light to reach the bottom and enables benthic macroflora to grow on nutrients of benthic origin. The decline of phytoplankton biomasses in shellfish farms induced a decrease in the nutrient demand, especially for ammonia. This situation was likely to favour nitrification, which led during autumn to higher nitrate concentrations within shellfish farming zones than outside. Therefore, filter feeders were able to alter the dominant biogeochemical process in the water column by stimulating nitrification. KEY WORDS: Suspension-feeding bivalves · Phytoplankton · Nutrient cycling · Carrying capacity · Thau lagoonResale or republication not permitted without written consent of the publisher

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Section: Methodsmentioning

confidence: 99%

Influence of shellfish farming activities on the biogeochemical composition of the water column in Thau lagoon

Souchu

Vaquer²,

Collos³

et al. 2001

Mar. Ecol. Prog. Ser.

148

108

View full text Add to dashboard Cite

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“…Samples for Chl-a measurement (100 to 1400 mL) were gently filtered onto glass-fiber filters (GF/F) and extracted in 90% acetone, before applying the fluorometric method of Holm-Hansen et al (1965) with a Kontron SFM25 fluorimeter. Samples were also fixed with a lugol-glutaraldehyde (1% vol-vol final concentration) and stored at 4°C for species identification and cell density measurements by inverted microscopy.…”

Section: Phytoplankton Composition and Biomassmentioning

confidence: 99%

Annual cycle of dimethylsulfoniopropionate (DMSP) and dimethylsulfoxide (DMSO) related to phytoplankton succession in the Southern North Sea

Speeckaert

Borges

Champenois

et al. 2018

Science of The Total Environment

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The influence of abiotic and biotic variables on the concentration of dimethyl sulfide (DMS), dimethylsulfoniopropionate (DMSP), and dimethylsulfoxide (DMSO), were investigated during an annual cycle in 2016 in the Belgian Coastal Zone (BCZ, North Sea). We reported strong seasonal variations in the concentration of these compounds linked to the phytoplankton succession with high DMS(P,O) producers (mainly Phaeocystis globosa) occurring in spring and low DMS(P,O) producers (various diatoms species) occurring in early spring and autumn. Spatial gradients of DMS and DMSP were related to those of phytoplankton biomass itself related to the inputs of nutrients from the Scheldt estuary. However, the use of a relationship with Chlorophyll-a (Chl-a) concentration is not sufficient to predict DMSP. Accounting for the phytoplankton composition, two different DMSP versus Chl-a correlations could be established, one for diatoms and another one for Phaeocystis colonies. We also reported high nearshore DMSO concentrations uncoupled to Chl-a and DMSP concentrations but linked to high suspended particulate matter (SPM) presumably coming from the Scheldt estuary as indicated by the positive relationship between annual average SPM and salinity.

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“…n s 9 and 0.28 " 0.005 nM n s 8 reported by Ž . pioneers Martin and Gordon 1988 Holm-Hansen et al 1965 . The phytoplankton species composition has been assessed in the parallel study by van Leeuwe et al Ž .…”

Section: Performancementioning

confidence: 99%

Low dissolved Fe and the absence of diatom blooms in remote Pacific waters of the Southern Ocean

et al. 1999

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The remote waters of the Pacific region of the Southern Ocean are the furthest away from any upstream and upwind Ž continental Fe sources. This prime area for expecting Fe limitation of the plankton ecosystem was studied March-April . 1995 along a north-south transect at ; 898W. At the end of the austral summer the upper wind-mixed layers were in the order of ; 100 m deep, thus mixing the algae down into the dimly lit part of the euphotic zone where photosynthesis is severely restricted. The dissolved Fe was found at low concentrations ranging from 0.05 nM near the surface to 0.5 nM in Ž . deeper waters. Along the transect 528S-698S , the dissolved iron was enhanced in the Polar Front, as well as near the Ž . Antarctic continental margin 0.6-1.0 nM . In between, the southern ACC branch was depleted with iron; here the concentrations in surface waters were quite uniform at about 0.21 nM. This is only somewhat lower than the 0.49 nM Ž . Ž . October 1992 and 0.31 nM November 1992 averages in early spring in the southern ACC part of Atlantic 68W sections wde Baar, H.J.W., de Jong, J.T.M., Bakker, D.C.E.. Loscher, B.M., Veth, C., Bathmann, U., Smetacek, V., 1995. Importancë of iron for phytoplankton spring blooms and CO drawdown in the Southern Ocean. Nature 373, 412-415; Loscher, B.M., 2 de Jong, J.T.M., de Baar, H.J.W., Veth, C., Dehairs, F., 1997. The distribution of iron in the Antarctic Circumpolar Current.x Deep-Sea Research II 44, 143-188. . First, the lower ; 0.21 nM in March-April 1995 may partly be due to continuation of the seasonal trend where the phytoplankton growth, albeit modest, was removing Fe from the surface waters. Secondly, the 898W Pacific stations are further downstream continental or seafloor sources than the Atlantic 68W section. In the latter case, the ACC water had passed through the Drake Passage and also over the Sandwich Plateau. Indeed for Drake Passage, intermediate Fe concentrations have been reported by others. The generally somewhat lower surface water Fe at the ACC and PF at 898W is consistent with the distance from sources and the late summer. It also would explain the very low Ž . abundance of phytoplankton Chl a in the region and the conspicuous absence of plankton blooms. In the subAntarctic waters north of the Polar Front there are no diatoms, let alone diatom blooms, due to low availability of silicate. Thus, it Baar et al.r Marine Chemistry 66 1999 1-34 2 appears the biological productivity is suppressed due to iron deficiency, in combination with the severe seasonal effects of wind mixing on the light climate, as well as regional silicate limitation for diatoms. q

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Fluorometric Determination of Chlorophyll

Cited by 2,225 publications

References 0 publications

Influence of shellfish farming activities on the biogeochemical composition of the water column in Thau lagoon

Influence of shellfish farming activities on the biogeochemical composition of the water column in Thau lagoon

Annual cycle of dimethylsulfoniopropionate (DMSP) and dimethylsulfoxide (DMSO) related to phytoplankton succession in the Southern North Sea

Low dissolved Fe and the absence of diatom blooms in remote Pacific waters of the Southern Ocean

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