Fluorometric Determination of Glucose by Flow Injection Analysis Using Immobilized Enzyme-Reactor Columns Prepared by Coupling Glucose Oxidase and Peroxidase onto Chitosan Beads

Taniai, Tetsuyuki; Sakuragawa, Akio; Okutani, Takashi

doi:10.2116/analsci.16.517

Cited by 10 publications

(4 citation statements)

References 13 publications

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“…In addition, the effectiveness of chitosan as a support can be associated with certain properties such as its elevated porosity, high hydrophilicity, large adhesion area, and thus, small mass transfer resistance to enzymes [1]. It has also been shown to be an efficient support for the immobilization of several enzymes such as glycosidases, proteases, glucose oxidase, and others [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Immobilization of a keratinolytic protease from Purpureocillium lilacinum on genipin activated-chitosan beads

Cavello

Contreras-Esquivel

Cavalitto

2014

Process Biochemistry

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Section: Introductionmentioning

confidence: 99%

Immobilization of a keratinolytic protease from Purpureocillium lilacinum on genipin activated-chitosan beads

Cavello

Contreras-Esquivel

Cavalitto

2014

Process Biochemistry

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“…It displays an excellent film-forming ability, good adhesion, biocompatibility, high mechanical strength, and a susceptibility to chemical modifications due to the presence of reactive hydroxyl and amino functional groups . It has been used previously as a support for immobilization of enzymes. − However, chitosan's applications in the development of enzyme-based amperometric biosensors have been very limited. − …”

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confidence: 99%

Integration of Enzymes and Electrodes: Spectroscopic and Electrochemical Studies of Chitosan−Enzyme Films

2002

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A new film-forming solution was developed for the efficient immobilization of enzymes on solid substrates. The solution consisted of a biopolymer, chitosan (CHIT), that was chemically modified with a permeability-controlling agent, Acetyl Yellow 9 (AY9), using glutaric dialdehyde (GDI) as a molecular tether. A model enzyme, glucose oxidase (GOx), was mixed with the CHIT-GDI-AY9 solution and cast on the surface of platinum electrodes to form robust CHIT-GDI-AY9-GOx films for glucose biosensing. UV-visible and infrared spectroscopies were used to determine the composition of the films. The optimized films contained on average 1 molecule of AY9/3 glucosamine units of chitosan and 25 free GDI tethers/1 molecule of GOx. The electrochemical assays of the films indicated both a very high efficiency of enzyme immobilization (approximately 99%) and large enzyme activity (60 units cm(-2)). The latter translated into a high sensitivity (42 mA M(-1) cm(-2)) of the Pt/CHIT-GDI-AY9-GOx biosensor toward glucose. The biosensor operated at 0.450 V, had a fast response time (t90% < or = 3 s), and was free of typical interferences, and its dynamic range covered 3 orders of magnitude of glucose concentrations. The lowest actually detectable concentration was 10 microM glucose. In addition, the biosensor displayed a practical shelf life and excellent operational stability, e.g. its response was stable during 24-h testing under continuous polarization and continuous flow of 5.0 mM glucose solution. The proposed approach to enzyme immobilization is simple, efficient, and cost-effective and should be of importance in the development of biosensors based on other enzymes that are more expensive than glucose oxidase.

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“…The derivatives or composites of these chitinous supports are also prepared as per the demand of biomedical or food industrial applications. Therefore, chitinous materials in various configurations such as powders, flakes, beads, membranes/films, capsules, fibers, sponges, lyposomes and sol-gels have been used as the support for enzyme immobilization [7,8,58]. Here we will discuss the CS and CT-derived composites and nanocomposites for enzyme immobilization and fabrication of biosensing devices for practical applications.…”

Section: Chitinous Scaffold For Immobilizationmentioning

confidence: 99%

“…This MPNFs-based novel sensor showed potential for glucose detection in blood and urine, which is indispensable for the diagnosis of diabetes [89]. In another study, HRP was coupled with GOx for preparation of a fluorometric FIA system for glucose determination in beverages, Japanese sake, and liquors [58]. Liposomes with entrapped GOx were prepared by covalent immobilization using GA-activated CS gel beads for a practical bioreactor application with high stability and reusability features [90].…”

Section: Gox-chitosan Electrochemistrymentioning

confidence: 99%

Exploration of Chitinous Scaffold-Based Interfaces for Glucose Sensing Assemblies

Bagal-Kestwal

Chiang

2019

Polymers

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The nanomaterial-integrated chitinous polymers have promoted the technological advancements in personal health care apparatus, particularly for enzyme-based devices like the glucometer. Chitin and chitosan, being natural biopolymers, have attracted great attention in the field of biocatalysts engineering. Their remarkable tunable properties have been explored for enhancing enzyme performance and biosensor advancements. Currently, incorporation of nanomaterials in chitin and chitosan-based biosensors are also widely exploited for enzyme stability and interference-free detection. Therefore, in this review, we focus on various innovative multi-faceted strategies used for the fabrication of biological assemblies using chitinous biomaterial interface. We aim to summarize the current development on chitin/chitosan and their nano-architecture scaffolds for interdisciplinary biosensor research, especially for analytes like glucose. This review article will be useful for understanding the overall multifunctional aspects and progress of chitin and chitosan-based polysaccharides in the food, biomedical, pharmaceutical, environmental, and other diverse applications.

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Fluorometric Determination of Glucose by Flow Injection Analysis Using Immobilized Enzyme-Reactor Columns Prepared by Coupling Glucose Oxidase and Peroxidase onto Chitosan Beads

Cited by 10 publications

References 13 publications

Immobilization of a keratinolytic protease from Purpureocillium lilacinum on genipin activated-chitosan beads

Immobilization of a keratinolytic protease from Purpureocillium lilacinum on genipin activated-chitosan beads

Integration of Enzymes and Electrodes: Spectroscopic and Electrochemical Studies of Chitosan−Enzyme Films

Exploration of Chitinous Scaffold-Based Interfaces for Glucose Sensing Assemblies

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