Fluorometric determination of okadaic acid using a truncated aptamer

Chemical Modification of Aptamers for Increased Binding Affinity in Diagnostic Applications: Current Status and Future Prospects

“…Both fluorescently-labeled and label-free biosensing approaches have been reported with the possibility of on-site detection using portable analyzers [ 56 , 57 , 58 , 59 ]. The target of interest can range from small molecules such as the mycotoxins aflatoxin B1 [ 60 ] and ochratoxin A [ 61 ], bisphenol A [ 62 ], the antibiotic tobramycin [ 54 ] and the marine toxin okadaic acid [ 63 ], to larger entities such as lipopolysaccharides [ 64 ] and the cyclic heptapeptide microcystin-LR (a hepatotoxin produced by cyanobacteria) [ 65 ]. Furthermore, proteins such as tropomyosin (a major shrimp allergen) [ 66 ] and glycated hemoglobin [ 67 ] or even whole bacteria such as Salmonella enteritidis [ 68 ] can be measured using this approach.…”

Selection and application of aptamers with high-affinity and high-specificity against dinophysistoxin-1

“…51 G-quadruplexes scoring of the N59af (the reverse complement of N59) revealed that N59af had the potential to form several kinds of Gquadruplexes structures, and N59a itself had the potential to form an i-motif fold structure by itself theoretically. 52,53 To verify these, we disrupted the arrangement mode of the bases. Therefore, N59a was truncated to obtain N59a2, N59a3, N59a4, N59a5, N59a6, N59a7 (Table S4 †).…”

An AuNPs-Based Fluorescent Sensor with Truncated Aptamer for Detection of Sulfaquinoxaline in Water

“…For example, Shi et al developed an SQX-specific aptamer after two truncations [ 4 ]. Although a new aptamer with a higher affinity was obtained, the method was cumbersome and required multiple cuts [ 4 , 17 ]. Therefore, an efficient and straightforward aptamers truncation approach is required.…”