Fluorometric enhancement of the detection of H<sub>2</sub>O<sub>2</sub>using different organic substrates and a peroxidase-mimicking polyoxometalate

Tian, Rui; Zhang, Boyu; Zhao, Mingming; Zou, Hangjin; Zhang, Chuhan; Ma, Qingjie

doi:10.1039/c9ra00505f

Cited by 20 publications

(9 citation statements)

References 44 publications

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“…The theoretical LOD was 1.0 mM, estimated using the 3sblank criterion. This LOD is higher than the LOD of silver NPs modified cellulose nanowhiskers [ 79 ], polyoxometalate [ 80 ], Ce 2 (WO 4 ) 3 , papain [ 81 ], Ag-nanoparticle-decorated silica microspheres [ 82 ] and magnetic mesoporous silica nanoparticles [ 83 ]. These results suggest that this material can be used to detect H 2 O 2 .…”

Section: Resultsmentioning

confidence: 99%

Synthesis of Gold-Platinum Core-Shell Nanoparticles Assembled on a Silica Template and Their Peroxidase Nanozyme Properties

Pham

Tran

Hahm

et al. 2022

IJMS

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Bimetallic nanoparticles are important materials for synthesizing multifunctional nanozymes. A technique for preparing gold-platinum nanoparticles (NPs) on a silica core template (SiO2@Au@Pt) using seed-mediated growth is reported in this study. The SiO2@Au@Pt exhibits peroxidase-like nanozyme activity has several advantages over gold assembled silica core templates (SiO2@Au@Au), such as stability and catalytic performance. The maximum reaction velocity (Vmax) and the Michaelis–Menten constants (Km) were and 2.1 × 10−10 M−1∙s−1 and 417 µM, respectively. Factors affecting the peroxidase activity, including the quantity of NPs, solution pH, reaction time, and concentration of tetramethyl benzidine, are also investigated in this study. The optimization of SiO2@Au@Pt NPs for H2O2 detection obtained in 0.5 mM TMB; using 5 µg SiO2@Au@Pt, at pH 4.0 for 15 min incubation. H2O2 can be detected in the dynamic liner range of 1.0 to 100 mM with the detection limit of 1.0 mM. This study presents a novel method for controlling the properties of bimetallic NPs assembled on a silica template and increases the understanding of the activity and potential applications of highly efficient multifunctional NP-based nanozymes.

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Section: Resultsmentioning

confidence: 99%

Synthesis of Gold-Platinum Core-Shell Nanoparticles Assembled on a Silica Template and Their Peroxidase Nanozyme Properties

Pham

Tran

Hahm

et al. 2022

IJMS

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“…Furthermore, Tian et al ( Tian et al, 2019 ) reported the synergetic combination of organic species such as benzoic acid (BA), thiamine (TH), and 3-(4-hydroxyphenyl)propionic acid (HPPA), with the Keggin-type polyoxotungstate intrinsic peroxidase Na 10 [ α -SiW 9 O 34 ] decomposing H 2 O 2 into ▪OH radicals, which converted weakly fluorescent substrates to strongly fluorescent substrates, under basic pH conditions. Recently, S-/N-containing ligands, such as 2,2′-dimethyl-4,4′-bithiazole ( Ying et al, 2019 ; Wang et al, 2020b ) and 2,5-bis(4-pyridyl)thiazolo [5,4- d ] thiazole ( Mou et al, 2020 ), and N-containing ligands, such as 2,2′-bipyridyl (bpy) and 4-(3-imidazol-1-yl-ethyl)-4H-[1,3,4]triazole (MET) ( Zhang et al, 2021a ) were used to modify a series of different archetype POM compounds, in order to build fluorescence sensors for Hg 2+ .…”

Section: Polyoxometalates Functionalized Sensorsmentioning

confidence: 99%

Polyoxometalate Functionalized Sensors: A Review

2022

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Polyoxometalates (POMs) are a class of metal oxide complexes with a large structural diversity. Effective control of the final chemical and physical properties of POMs could be provided by fine-tuning chemical modifications, such as the inclusion of other metals or non-metal ions. In addition, the nature and type of the counterion can also impact POM properties, like solubility. Besides, POMs may combine with carbon materials as graphene oxide, reduced graphene oxide or carbon nanotubes to enhance electronic conductivity, with noble metal nanoparticles to increase catalytic and functional sites, be introduced into metal-organic frameworks to increase surface area and expose more active sites, and embedded into conducting polymers. The possibility to design POMs to match properties adequate for specific sensing applications turns them into highly desirable chemicals for sensor sensitive layers. This review intends to provide an overview of POM structures used in sensors (electrochemical, optical, and piezoelectric), highlighting their main functional features. Furthermore, this review aims to summarize the reported applications of POMs in sensors for detecting and determining analytes in different matrices, many of them with biochemical and clinical relevance, along with analytical figures of merit and main virtues and problems of such devices. Special emphasis is given to the stability of POMs sensitive layers, detection limits, selectivity, the pH working range and throughput.

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“…The absorbance values were increased with an increasing amount of NFs and length of time. According to a method described previously [26][27][28][29][30], the mechanism of peroxidase-like catalytic activities of Hb-Cu 3 (PO 4 ) 2 NFs can be further investigated using steady-state kinetic assays at pH 10. As shown in Figure S6, a typical Michaelis-Menten curve was obtained for Hb-Cu 3 (PO 4 ) 2 NFs in the presence of H 2 O 2 and OPD, respectively.…”

Section: Enzyme-like Activities and Kinetic Parameters Of Hb-cu 3 (Po 4 ) 2 Nfsmentioning

confidence: 99%

Fluorometric Detection of Thiamine Based on Hemoglobin–Cu3(PO4)2 Nanoflowers (NFs) with Peroxidase Mimetic Activity

Zou

Zhang

et al. 2020

Sensors

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Component analysis plays an important role in food production, pharmaceutics and agriculture. Nanozymes have attracted wide attention in analytical applications for their enzyme-like properties. In this work, a fluorometric method is described for the determination of thiamine (TH) (vitamin B1) based on hemoglobin–Cu3(PO4)2 nanoflowers (Hb–Cu3(PO4)2 NFs) with peroxidase-like properties. The Hb–Cu3(PO4)2 NFs catalyzed the decomposition of H2O2 into ·OH radicals in an alkaline solution that could efficiently react with nonfluorescent thiamine to fluoresce thiochrome. The fluorescence of thiochrome was further enhanced with a nonionic surfactant, Tween 80. Under optimal reaction conditions, the linear range for thiamine was from 5 × 10−8 to 5 × 10−5 mol/L. The correlation coefficient for the calibration curve and the limit of detection (LOD) were 0.9972 and 4.8 × 10−8 mol/L, respectively. The other vitamins did not bring about any obvious changes in fluorescence. The developed method based on hybrid nanoflowers is specific, pragmatically simple and sensitive, and has potential for application in thiamine detection.

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Fluorometric enhancement of the detection of H₂O₂using different organic substrates and a peroxidase-mimicking polyoxometalate

Abstract: A fluorometric enhancement method based on a polyoxotungstate intrinsic peroxidase for the detection of hydrogen peroxide under basic conditions is developed for the first time.

Cited by 20 publications

References 44 publications

Synthesis of Gold-Platinum Core-Shell Nanoparticles Assembled on a Silica Template and Their Peroxidase Nanozyme Properties

Synthesis of Gold-Platinum Core-Shell Nanoparticles Assembled on a Silica Template and Their Peroxidase Nanozyme Properties

Polyoxometalate Functionalized Sensors: A Review

Fluorometric Detection of Thiamine Based on Hemoglobin–Cu3(PO4)2 Nanoflowers (NFs) with Peroxidase Mimetic Activity

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Fluorometric enhancement of the detection of H2O2using different organic substrates and a peroxidase-mimicking polyoxometalate

Abstract: A fluorometric enhancement method based on a polyoxotungstate intrinsic peroxidase for the detection of hydrogen peroxide under basic conditions is developed for the first time.

Cited by 20 publications

References 44 publications

Synthesis of Gold-Platinum Core-Shell Nanoparticles Assembled on a Silica Template and Their Peroxidase Nanozyme Properties

Synthesis of Gold-Platinum Core-Shell Nanoparticles Assembled on a Silica Template and Their Peroxidase Nanozyme Properties

Polyoxometalate Functionalized Sensors: A Review

Fluorometric Detection of Thiamine Based on Hemoglobin–Cu3(PO4)2 Nanoflowers (NFs) with Peroxidase Mimetic Activity

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Fluorometric enhancement of the detection of H₂O₂using different organic substrates and a peroxidase-mimicking polyoxometalate