Fluorometric Method for Determination of Cholesterol in Microliter Quantities of Blood

Tishler, Frederick; Bathish, J. N.

doi:10.1002/jps.2600541219

Search citation statements

Order By: Relevance

Paper Sections

Select...

By Fluorescence Characteristics1

Organic and Biological1

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1967

1978

Publication Types

Select...

Article7

Relationship

Self Cite0

Independent7

Authors

Journals

Cited by 9 publications

(2 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…was evaporated to dryness under a stream of nitrogen at 37°, the residue dissolved in chloroform (2 ml. ), and the total cholesterol concentration measured by the fluorimetrie method of Tishler & Bathish (1965).…”

Section: By Fluorescence Characteristicsmentioning

confidence: 99%

The Interference of Free and Esterified Cholesterol in the Spectrofluorimetric Measurement of Plasma 11-Hydroxysteroids

Stenlake¹,

Davidson²,

Williams³

et al. 1970

Journal of Endocrinology

View full text Add to dashboard Cite

Cholesterol and cholesterol esters have been identified on the basis of thin-layer and gas\p=m-\liquid chromatographic data and fluorimetric characteristics as major interfering fluorogens in a simple fluorimetric method for determining plasma corticosteroids.In a small series ofnormal subjects the average concentration of fluorogen, which was not 11-hydroxysteroid, was 3\m=.\4 \g=m\g.apparent cortisol/100 ml. plasma, equivalent to 22\m=.\4 % of the total fluorogen present.

show abstract

Section: By Fluorescence Characteristicsmentioning

confidence: 99%

The Interference of Free and Esterified Cholesterol in the Spectrofluorimetric Measurement of Plasma 11-Hydroxysteroids

Stenlake¹,

Davidson²,

Williams³

et al. 1970

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…Fluorometric analyses of cholesterol in blood made use of the fluorescence developed in chloroform solution by treatment with acetyl choride-zinc chloride reagent (654) or acetic anhydride followed by sulfuric acid (740); an automated procedure has also been described (335). Sterol acetate spots on thin layer chromatograms fluoresce brightly after spraying with 0.2% ethanolic dibromofluorescein (138).…”

Section: Organic and Biologicalmentioning

confidence: 99%