Fluorometric procedures for dye tracing

Numerical Simulations and Tracer Studies as a Tool to Support Water Circulation Modeling in Breeding Reservoirs

“…Over the last several decades, only two dyes (variations of the same molecular structure called xanthene), have been used as fluorescent substances in such studies: intracid rhodamine B and rhodamine WT. Rhodamine WT is preferred for most fluorometry studies because of its ease of use, relatively low cost, low adsorption tendency, strong fluorescent properties, high diffusivity, chemical stability, and limited impact on the aquatic environment (Parker 1973, Smart and Laidlaw 1977, Wilson et al 1986, Kilpatrick and Wilson 1988. In addition, the spectral characteristics of rhodamine WT are quite different from those of most other substances in the surrounding aqueous environment, making it ideal for use as a marker in tracer studies of water and wastewater (Wilson et al 1986, Zima 2012.…”

“…In the case of breeding tanks, it is very important to select a tracer (marker) that does not affect the animals living there. Such a substance is rhodamine WT, commonly used in the natural environment (Parker 1973, Smart and Laidlaw 1977, Wilson et al 1986, Kilpatrick and Wilson 1989, Zima 2012. In a tracer study, dye molecules move along with water molecules and undergo a process of diffusion.…”

Automatic dilution gaging of rapidly varying flow

“…The procedures used for analyses are described by Wilson (1968). percent, with an average difference of -1.4 percent.…”

Traveltime and reaeration characteristics for a reach of the Rio Grande, Albuquerque, New Mexico, October 1991

“…Dye concentrations were analyzed using fluorometric procedures presented by Wilson (1968). Calibration curves were determined from dye standards prepared from the dye lot used.…”