Fluorometric reaction rate method for the determination of thiamine

Ryan, Mary Andrieu; Ingle, J. D.

doi:10.1021/ac50063a042

Cited by 61 publications

(21 citation statements)

References 29 publications

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“…The fact that thiamine hydrochloride was used in the present study rules out the possibility that Pb interacts with phosphate group at pH 6 and subtle changes in the absorption properties, particularly the hypochromic shift in pyrimidine absorption strongly suggests its involvement in interaction with lead, which could be mediated through its N1 atom (Stamatis et al 2007;Malandrinos et al 2006). Oxidation of thiamine in the presence of mercury (Hg) leading to the formation of fluorescence thiamine adduct has been used to measure the Hg or thiamine levels (Malandrinos et al 2006;SeguraCarretero et al 1999;Ryan and Ingle 1980). Furthermore, reduction of Hg (?2) is proposed to be catalyzed by the pyrimidine ring of thiamine (Ryan and Ingle 1980).…”

Section: Resultsmentioning

confidence: 99%

“…Oxidation of thiamine in the presence of mercury (Hg) leading to the formation of fluorescence thiamine adduct has been used to measure the Hg or thiamine levels (Malandrinos et al 2006;SeguraCarretero et al 1999;Ryan and Ingle 1980). Furthermore, reduction of Hg (?2) is proposed to be catalyzed by the pyrimidine ring of thiamine (Ryan and Ingle 1980). Similarly, addition of Hg resulted in the oxidation of thiamine, as evidenced by increased fluorescence emission at 444 nm.…”

Section: Resultsmentioning

confidence: 99%

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Thiamine reduces tissue lead levels in rats: mechanism of interaction

2009

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Lead (Pb) toxicity has been a serious concern in industrialized societies because of its association with functional deficits in nervous, haematopoietic and renal systems. Several studies have shown beneficial effects of thiamine on Pb toxicity. It is speculated that Pb chelation by thiamine may be a possible mechanism. However, the exact nature of these interactions remained elusive. In the present study we have characterized the interaction of Pb with thiamine using UV-Vis as well as fluorescence spectroscopic methods and studied the effect of thiamine treatment on blood and tissue Pb levels during simultaneous or post-exposure to Pb in rat model. The spectroscopic studies revealed that Pb interacts with the pyrimidine ring of thiamine, leading to its solubilization at physiological pH. Further, thiamine reduced the Pb levels in blood, kidney and bone during both simultaneous and post-exposure Pb treatment. Interestingly, thiamine appears to prevent the accumulation of Pb in bone during simultaneous treatment. Together these results suggest that pyrimidine ring of thiamine mediates its interaction with Pb, leading to the prevention of its accumulation and/or increased clearance from tissues.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Thiamine reduces tissue lead levels in rats: mechanism of interaction

2009

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“…The ratio of TC to TDS is affected by the pH, solvent, and oxidizing agent. 15 Reports show that in the oxidation of thiamine by H2O2 under a catalysis of HRP, 16 FeTSPc led to a high yield of TC. 17 Similarly, as a mimetic enzyme of HRP, Hb can also catalyze the oxidation of TM by ONOO -to produce TC in a basic medium with a high yield.…”

Section: Reaction Mechanism Of Thiamine With Onoo -mentioning

confidence: 99%

Fluorimetric Determination of Peroxynitrite Based on an Enzymatic Reaction

et al. 2005

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“…11 The most widely used method for the assay of thiamine is the spectrofluorimetric method, which involves the oxidation of thiamine to form fluorescent thiochrome (TC). This reaction is always accompanied by the simultaneous formation of nonfluorescent compound, thiamine disulfide (TDS).…”

Section: Introductionmentioning

confidence: 99%

The Use of Crude Extract of Kohlrabi (Brassica oleracea gongylodes) as a Source of Peroxidase in the Spectrofluorimetric Determination of Thiamine

Amjadi

Manzoori²,

Orooji³

2007

Bulletin of the Korean Chemical Society

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The crude extract of kohlrabi (Brassica oleracea gongylodes) was prepared as a rich source of peroxidase and its enzymatic and specific activities in the presence of cross-linked polyvinylpyrrolidone as a stabilizer were determined. This extract was used to catalyze the oxidation of thiamine in the presence of hydrogen peroxide to thiochrome at pH 8.0. Thiochrome shows strong fluorescence at 436 nm with excitation at 370 nm. Based on the obtained results, a sensitive and simple spectrofluorimetric method was developed for the determination of thiamine. In the optimum conditions, the calibration graph was linear from 2 × 10 −7 to 1 × 10 −4 mol L −1, with a detection limit of 6.2 × 10 −8 mol L −1. The relative standard deviation (RSD) was 1.2% for 5 × 10 −6 mol L −1 thiamine. The method was successfully applied to the determination of thiamine in vitamin B1 and vitamin B complex tablets and vitamin B complex syrup.

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Fluorometric reaction rate method for the determination of thiamine

Cited by 61 publications

References 29 publications

Thiamine reduces tissue lead levels in rats: mechanism of interaction

Thiamine reduces tissue lead levels in rats: mechanism of interaction

Fluorimetric Determination of Peroxynitrite Based on an Enzymatic Reaction

The Use of Crude Extract of Kohlrabi (Brassica oleracea gongylodes) as a Source of Peroxidase in the Spectrofluorimetric Determination of Thiamine

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