Fluorophores: Descriptions and Properties

Albani, J.R.

doi:10.1016/b978-044451449-3/50003-4

Cited by 9 publications

(11 citation statements)

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“…Galbán et al [26] reported the average fluorescence lifetime of riboflavin to be 5.06 ns, FMN 4.7 to be ns, and FAD to be 2.27 ns. This is supported by Albani, who stated that τ FAD = 2.3 ns and τ FMN = 4.7 ns [27]. Although we did not get the same discernment in this particular experiment, we do strongly agree with these values because of the average fluorescence lifetimes we concluded to be connected with flavins in the cells (see Section 3.3).…”

Section: Analysis Of Emission Spectra Of Flavins and Whole Bacterial ...supporting

confidence: 84%

“…As was written in Section 3.1, one team led by Galbán [26] reported the average fluorescence lifetime of riboflavin to be 5.06 ns, FMN to be 4.7 ns, and FAD to be 2.27 ns. The second team, led by Albani, supported these values, and their experiments yielded values of τFAD = 2.3 ns and τFMN = 4.7 ns [27]. We strongly agree with these values because of the average fluorescence lifetimes we concluded to be connected with flavins in the cells (see Section 3.3).…”

Section: Analysis Of Emission Spectra Of Flavins and Whole Bacterial ...supporting

confidence: 71%

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Use of Flavin-Related Cellular Autofluorescence to Monitor Processes in Microbial Biotechnology

2022

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Cellular autofluorescence is usually considered to be a negative phenomenon because it can affect the sensitivity of fluorescence microscopic or flow cytometric assays by interfering with the signal of various fluorescent probes. Nevertheless, in our work, we adopted a different approach, and green autofluorescence induced by flavins was used as a tool to monitor fermentation employing the bacterium Cupriavidus necator. The autofluorescence was used to distinguish microbial cells from abiotic particles in flow cytometry assays, and it was also used for the determination of viability or metabolic characteristics of the microbial cells. The analyses using two complementary techniques, namely fluorescence microscopy and flow cytometry, are simple and do not require labor sample preparation. Flavins and their autofluorescence can also be used in a combination with other fluorophores when the need for multi-parametrical analyses arises, but it is wise to use dyes that do not emit a green light in order to not interfere with flavins’ emission band (500–550 nm).

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Section: Analysis Of Emission Spectra Of Flavins and Whole Bacterial ...supporting

confidence: 84%

Section: Analysis Of Emission Spectra Of Flavins and Whole Bacterial ...supporting

confidence: 71%

Use of Flavin-Related Cellular Autofluorescence to Monitor Processes in Microbial Biotechnology

2022

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“…The lectin did not show autofluorescence, since lectins that present saccharides are non-fluorescent molecules [59]. On the other hand, the analysis of the QD alone showed one peak of fluorescence spectral emission at 570 nm.…”

Section: Discussionmentioning

confidence: 89%

Quantum Dot Labelling of Tepary Bean (Phaseolus acutifolius) Lectins by Microfluidics

et al. 2020

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Lectins are bioactive proteins with the ability to recognize cell membrane carbohydrates in a specific way. Diverse plant lectins have shown diagnostic and therapeutic potential against cancer, and their cytotoxicity against transformed cells is mediated through the induction of apoptosis. Previous works have determined the cytotoxic activity of a Tepary bean (Phaseolus acutifolius) lectin fraction (TBLF) and its anti-tumorigenic effect on colon cancer. In this work, lectins from the TBLF were additionally purified by ionic-exchange chromatography. Two peaks with agglutination activity were obtained: one of them was named TBL-IE2 and showed a single protein band in two-dimensional electrophoresis; this one was thus selected for coupling to quantum dot (QD) nanoparticles by microfluidics (TBL-IE2-QD). The microfluidic method led to low sample usage, and resulted in homogeneous complexes, whose visualization was achieved using multiphoton and transmission electron microscopy. The average particle size (380 nm) and the average zeta potential (−18.51 mV) were determined. The cytotoxicity of the TBL-IE2 and TBL-IE2-QD was assayed on HT-29 colon cancer cells, showing no differences between them (p ≤ 0.05), where the LC50 values were 1.0 × 10−3 and 1.7 × 10−3 mg/mL, respectively. The microfluidic technique allowed control of the coupling between the QD and the protein, substantially improving the labelling process, providing a rapid and efficient method that enabled the traceability of lectins. Future studies will focus on the potential use of the QD-labelled lectin to recognize tumor tissues.

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“…However, porphyrins like heme precursors and other derivatives do possess inherent fluorescence when lacking the quenching iron atoms, typically exhibiting emission peaks in the 620-635 nm range when excited at 405 nm (Fauaz et al, 2010) (Figure 2c,d). This porphyrin fluorescence is especially notable when the Wetlaufer, 1963), (b) Emission spectra (redrawn from Albani, 2004) of aromatic amino acids tryptophan, tyrosine, and phenylaniline. (c) Excitation spectra and (d) emission spectra of collagen (black), elastin (blue), porphyrins (red), lipo-pigments (cyan) and pyridoxine (green) (redrawn from Wagnieres et al, 1998).…”

Section: Different Components Of the Blood And Their Source Of Autofl...mentioning

confidence: 99%

“…Absorption, emission, and excitation spectra endogenous fluorophores. (a) Absorption (redrawn from Wetlaufer, 1963), (b) Emission spectra (redrawn from Albani, 2004) of aromatic amino acids tryptophan, tyrosine, and phenylaniline. (c) Excitation spectra and (d) emission spectra of collagen (black), elastin (blue), porphyrins (red), lipo‐pigments (cyan) and pyridoxine (green) (redrawn from Wagnieres et al, 1998).…”

Section: Source Of Autofluorescence Of Bloodmentioning

confidence: 99%

Autofluorescence of blood and its application in biomedical and clinical research

Shrirao

Schloss

Fritz

et al. 2021

Biotech & Bioengineering

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Autofluorescence of blood has been explored as a label free approach for detection of cell types, as well as for diagnosis and detection of infection, cancer, and other diseases.Although blood autofluorescence is used to indicate the presence of several physiological abnormalities with high sensitivity, it often lacks disease specificity due to use of a limited number of fluorophores in the detection of several abnormal conditions. In addition, the measurement of autofluorescence is sensitive to the type of sample, sample preparation, and spectroscopy method used for the measurement. Therefore, while current blood autofluorescence detection approaches may not be suitable for primary clinical diagnosis, it certainly has tremendous potential in developing methods for large scale screening that can identify high risk groups for further diagnosis using highly specific diagnostic tests. This review discusses the source of blood autofluorescence, the role of spectroscopy methods, and various applications that have used autofluorescence of blood, to explore the potential of blood autofluorescence in biomedical research and clinical applications.

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Fluorophores: Descriptions and Properties

Cited by 9 publications

References 0 publications

Use of Flavin-Related Cellular Autofluorescence to Monitor Processes in Microbial Biotechnology

Use of Flavin-Related Cellular Autofluorescence to Monitor Processes in Microbial Biotechnology

Quantum Dot Labelling of Tepary Bean (Phaseolus acutifolius) Lectins by Microfluidics

Autofluorescence of blood and its application in biomedical and clinical research

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