Fluorophotometric determination of critical micelle concentration (CMC) of ionic and non-ionic surfactants with carbon dots via Stokes shift

Bhaisare, Mukesh Lavkush; Pandey, Sunil; Khan, M. Shahnawaz; Talib, Abou; Wu, Hui‐Fen

doi:10.1016/j.talanta.2014.09.011

Cited by 53 publications

(10 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compounds 2 and 3 show a marked emission intensity increase when the critical micelle concentration (cmc) of SDS is reached. This is reported to be 7.3 mM in water and 1.1 mM in PBS, and therefore at higher concentrations of SDS in PBS (or purified water, data not shown) a significant increase in emission intensity compared to lower concentrations can be observed (Figure S4B). In contrast, probes 4 and 5 already show a partial intensity increase at lower SDS concentrations well below the cmc (in PBS).…”

Section: Resultsmentioning

confidence: 74%

Exploring the Influence of Different Albumin Binders on Molecular Imaging Probe Distribution

et al. 2021

View full text Add to dashboard Cite

The biodistribution of molecular imaging probes or tracers mainly depends on the chemical nature of the probe and the preferred metabolization and excretion routes. Small molecules have rather short half-lives while antibodies reside inside the organism for a longer period of time. An excretion via kidneys and bladder is faster than a mainly hepatobiliary elimination. To manipulate the biodistribution behavior of probes, different strategies have been pursued, including utilizing serum albumin as an inherent transport mechanism for small molecules. Here, we modified an existing small molecular fluorescent probe targeted to the endothelin-A receptor (ET A R) with three different albumin-binding moieties to search for an optimal modification strategy. A diphenylcyclohexyl (DPCH) group, a p-iodophenyl butyric acid (IPBA), and a fatty acid (FA) group were attached via amino acid linkers. All three modifications result in transient albumin binding of the developed compounds, as concluded from gel electrophoresis investigations. Spectrophotometric measurements applying variable amounts of bovine, murine, and human serum albumin (BSA, MSA, and HSA) reveal distinct variations of absorption and emission intensities and shifts of their maximum wavelengths. Binding to MSA results in the weakest effects, while binding to HSA leads to the strongest. Cell-based in vitro investigations utilizing ET A R-positive HT-1080 fibrosarcoma and ET A R-negative BT-20 breast adenocarcinoma cells support a retained specific target-binding capacity of the modified compounds and different degrees of unspecific binding. In vivo analysis of a HT-1080 xenograft model in nude mice over the course of 1 week by fluorescence reflectance imaging illustrates noticeable differences between the four examined probes. While the IPBA-modified probe shows the highest absolute signal intensity values, the FA-modified probe exhibits the most favorable tumor-to-organ ratios. In summary, reversible binding to albumin enhances the biological half-life of the designed probes substantially and enables near infrared optical imaging of subcutaneous tumors for several days in vivo. Because the unmodified probe already exhibits reasonable results, the attachment of albumin-binding moieties does not lead to a substantially improved imaging outcome in terms of target-to-background ratios. On the other hand, because the implemented transient albumin binding results in an overall higher amount of probe inside tumor lesions, this strategy might be adaptable for theranostic or therapeutic approaches in a future clinical routine.

show abstract

Section: Resultsmentioning

confidence: 74%

Exploring the Influence of Different Albumin Binders on Molecular Imaging Probe Distribution

et al. 2021

View full text Add to dashboard Cite

show abstract

“…However, SDS lysis buffer with concentrations greater than 0.02% had dramatically higher DAPI fluorescence. SDS is known to form micelles at certain SDS concentration depending on the solution properties [37]. Inside these micelles, hydrophobic conditions, which could increase DAPI fluorescence intensity, are formed [38].…”

Section: Discussionmentioning

confidence: 99%

DNA-DAPI Interaction-Based Method for Cell Proliferation Rate Evaluation in 3D Structures

Šimoliūnas

Kantakevičius

Kalvaitytė

et al. 2021

CIMB

View full text Add to dashboard Cite

Effective cell number monitoring throughout the three-dimensional (3D) scaffold is a key factor in tissue engineering. There are many methods developed to evaluate cell number in 2D environments; however, they often encounter limitations in 3D. Therefore, there is a demand for reliable methods to measure cell proliferation in 3D surroundings. Here, we report a novel technique for the DNA content-based evaluation of cell proliferation using DNA-binding dye DAPI. We demonstrated the method’s compatibility with four different cell cultures: cancer lines MCF-7 and MH-22a, embryonic fibroblast cell line Swiss 3T3, and primary mesenchymal stem cell culture isolated from rat’s incisors. The DAPI based method was able to successfully evaluate cell proliferation in 2D, 2.5D, and 3D environments. Even though the proposed method does not discriminate between viable and dead cells, it might give a convenient snapshot of the cell number at a given time point. This should help to more reliably evaluate various processes proceeding in 2.5D and 3D cultures.

show abstract

“…The results suggest that hydrogen bond was formed with water molecules from the hydration layer on the COO -group of each amphiphilic chitosan derivatives chain [22]. Furthermore, the association of the hydrophobic group in OCMCs, DCMCs and DACMCs around pyrene molecules had enhanced the intensity of peak I272 [19]. The CMC value of OCMCs, DCMCs and DACMCs was determined according to a method outlined by Aguair et al [23] by plotting the intensity ratios of the first peak (I372, 372 nm) and the third peak (III384, 384 nm).…”

Section: Critical Micelles Concentration (Cmc)mentioning

confidence: 91%

“…CMC occurs when the concentration reached a particular ranged where molecules started to form self-assembled [19]. The CMC value of OCMCs, DCMCs and DACMCs was determined by using pyrene as a hydrophobic probe.…”

Section: Critical Micelles Concentration (Cmc)mentioning

confidence: 99%

Amphiphilic chitosan derivatives as carrier agents for rotenone

Kamari

Aljafree

2017

AIP Conference Proceedings

View full text Add to dashboard Cite

Abstract.In the present study, the feasibility of amphiphilic chitosan derivatives, namely oleoyl carboxymethyl chitosan (OCMCs), N,N-dimethylhexadecyl carboxymethyl chitosan (DCMCs) and deoxycholic acid carboxymethyl chitosan (DACMCs) as carrier agents for rotenone in water-insoluble pesticide formulations was investigated. Fourier Transform Infrared (FTIR) Spectrometer, CHN-O Elemental Analyser (CHN-O) and Transmission Electron Microscope (TEM) were used to characterise amphiphilic chitosan derivatives. The critical micelle concentration (CMC) of amphiphilic chitosan derivatives was determined using a Fluorescence Spectrometer. A High Performance Liquid Chromatography (HPLC) was used to determine the ability of OCMCs, DCMCs and DACMCs to load and release rotenone in an in vitro system. Based on TEM analysis, results have shown that amphiphilic chitosan derivatives formed self-assembly and exhibited spherical shape. The CMC values determined for OCMCs, DCMCs and DACMCs were 0.093, 0.098 and 0.468 mg/mL, respectively. The encapsulation efficiency (EE) values for the materials were more than 97.0%, meanwhile the loading capacity (LC) values were greater than 0.90%. OCMCs, DCMCs and DACMCs micelles exhibited an excellent ability to control the release of rotenone, of which 90.0% of rotenone was released within 40 to 52 h. In conclusion, OCMCs, DCMCs and DACMCs possess several key features to act as effective carrier agents for rotenone. Overall, amphiphilic chitosan derivatives produced in this study were successfully increased the solubility of rotenone by 49.0 times higher than free rotenone.

show abstract

Fluorophotometric determination of critical micelle concentration (CMC) of ionic and non-ionic surfactants with carbon dots via Stokes shift

Cited by 53 publications

References 34 publications

Exploring the Influence of Different Albumin Binders on Molecular Imaging Probe Distribution

Exploring the Influence of Different Albumin Binders on Molecular Imaging Probe Distribution

DNA-DAPI Interaction-Based Method for Cell Proliferation Rate Evaluation in 3D Structures

Amphiphilic chitosan derivatives as carrier agents for rotenone

Contact Info

Product

Resources

About