Fluoroquinolone-resistant and extended-spectrum β-lactamase-producing Escherichia coli from the milk of cows with clinical mastitis in Southern Taiwan

Su, Yao-Chi; Yu, Chang-You; Tsai, Yi Lin; Wang, Shao–Hung; Lee, Chihan; Chu, Chishih

doi:10.1016/j.jmii.2014.10.003

Cited by 60 publications

(47 citation statements)

References 49 publications

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“…The RmtD variant has been found less frequently. Other than the report from Yang et al (123), another recent report has been published from Brazil, on one E. coli isolate from a diseased horse producing RmtD and harboring the bla CTX-M- 15 and aac(6′)-Ib-cr genes (125). The RmtE methylase was reported for the first time from commensal E. coli isolates from healthy calves in the United States (126).…”

Section: Resistance To Aminoglycosides By Target Modificationsmentioning

confidence: 99%

“…To date, the global picture indicates that antimicrobial susceptibility of E. coli in mastitis remains high. In particular, extended-spectrum β-lactamases (ESBLs) or overexpressed cephalosporinases (AmpCs) produced by E. coli and conferring resistance to broad-spectrum cephalosporins have been sporadically isolated from milk samples (13)(14)(15)(16). Those families of antimicrobial agents may also be prescribed in newborns affected by diarrhea.…”

Section: E Coli In Animals: a Pathogenic And A Commensal Bacteriummentioning

confidence: 99%

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Antimicrobial Resistance inEscherichia coli

et al. 2018

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Multidrug resistance in has become a worrying issue that is increasingly observed in human but also in veterinary medicine worldwide. is intrinsically susceptible to almost all clinically relevant antimicrobial agents, but this bacterial species has a great capacity to accumulate resistance genes, mostly through horizontal gene transfer. The most problematic mechanisms in correspond to the acquisition of genes coding for extended-spectrum β-lactamases (conferring resistance to broad-spectrum cephalosporins), carbapenemases (conferring resistance to carbapenems), 16S rRNA methylases (conferring pan-resistance to aminoglycosides), plasmid-mediated quinolone resistance (PMQR) genes (conferring resistance to [fluoro]quinolones), and genes (conferring resistance to polymyxins). Although the spread of carbapenemase genes has been mainly recognized in the human sector but poorly recognized in animals, colistin resistance in seems rather to be related to the use of colistin in veterinary medicine on a global scale. For the other resistance traits, their cross-transfer between the human and animal sectors still remains controversial even though genomic investigations indicate that extended-spectrum β-lactamase producers encountered in animals are distinct from those affecting humans. In addition, of animal origin often also show resistances to other-mostly older-antimicrobial agents, including tetracyclines, phenicols, sulfonamides, trimethoprim, and fosfomycin. Plasmids, especially multiresistance plasmids, but also other mobile genetic elements, such as transposons and gene cassettes in class 1 and class 2 integrons, seem to play a major role in the dissemination of resistance genes. Of note, coselection and persistence of resistances to critically important antimicrobial agents in human medicine also occurs through the massive use of antimicrobial agents in veterinary medicine, such as tetracyclines or sulfonamides, as long as all those determinants are located on the same genetic elements.

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Section: Resistance To Aminoglycosides By Target Modificationsmentioning

confidence: 99%

Section: E Coli In Animals: a Pathogenic And A Commensal Bacteriummentioning

confidence: 99%

Antimicrobial Resistance inEscherichia coli

et al. 2018

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“…The second continent with the highest number of countries reporting E-ESBL in cattle is Asia, which shows description of E-ESBL in 13 countries (China, Hong Kong, India, Indonesia, Iran, Israel, Japan, Lebanon, Malaysia, Saudi Arabia, South Korea, Thailand and Taiwan), highlighting China and India which are the fourth and fifth largest cattle producers in the world (Adler et al, 2015;Ali et al, 2017;Barzan et al, 2017;Diab et al, 2016;Hassan et al, 2015;Hinthong et al, 2017;Kamaruzzaman, 2015;Koovapra et al, 2016;Ohnishi et al, 2013a;Su et al, 2016;Sudarwanto et al, 2016;Tark et al, 2017). They have different roles in the import and export scenario, as India is the world's largest exporter of cattle and China the largest importer, since its internal production is not sufficient for internal consumption (USDA et al, 2017).…”

Section: Asiamentioning

confidence: 99%

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

Palmeira

Ferreira

2020

Heliyon

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Food producing animal is a global challenge in terms of antimicrobial resistance spread. Extended-spectrum betalactamase (ESBL)-producing Enterobacteriaceae are relevant opportunistic pathogens that may spread in many ecological niches of the One Health approach as human, animal and environment due to intestinal selection of antimicrobial resistant commensals in food production animals. Cattle production is a relevant ecological niche for selection of commensal bacteria with antimicrobial resistance from microbiota. Enterobacteriaceae show importance in terms of circulation of resistant-bacteria and antimicrobial resistance genes via food chain creating a resistance reservoir, setting up a threat for colonization of humans and consequent health risk. ESBL-producing Enterobacteriaceae are a threat in terms of human health responsible for life threatening outbreaks and silent enteric colonization of community populations namely the elder population. Food associated colonization is a risk difficult to handle and control. In a time of globalization of food trading, population intestinal colonization is a mirror of food production and in that sense this work aims to make a picture of ESBL-producing Enterobacteriaceae in animal production for food over the world in order to make some light in this reality of selection of resistant threats in food producing animal.

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“…Most genes encoding this type of antimicrobial resistance are located in conjugative plasmids or integron systems, and could be exchanged readily between enterobacteria species, facilitating their dissemination (CARATTOLI, 2013;WOODFORD et al, 2011). Current studies have reported the presence of ESBL producing E. coli in food, such as meat, milk and different types of cheese (GUNDOGAN;AVCI, 2013;SU et al, 2016;TEKINER;OZPINAR, 2016;VRABEC et al, 2015). Once beta-lactam resistant E. coli strains have become a global emerging problem in the last few years, carbapenems have been one of the main therapy options against human infections, including those caused by ESBL-producing bacteria (GENC et al, 2016;SPELLBERG et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Detection of virulence genes and antimicrobial resistance profiles of Escherichia coli isolates from raw milk and artisanal cheese in Southern Brazil

Parussolo

Sfaciotte

Dalmina

et al. 2019

SCA

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The serrano artisanal cheese is a typical product from South region of Brazil, which is produced by skilled cheesemakers using raw milk. The contamination of this food by Escherichia coli has a great impact on public health, since it could threat the consumers’ health. The study evaluated the presence of virulence genes, antimicrobial susceptibility profiles and bofilm-production ability of Escherichia coli isolates obtained from raw milk and artisanal cheese produced in Southern Brazil. A total of 117 isolates of E. coli were characterized by multiplex PCR to detect the following virulence genes: eae for enteropatogenic E. coli (EPEC), lt and st for enterotoxigenic E. coli (ETEC), stx for shiga toxin-producing E. coli (STEC), stx and eae for enterohemorrhagic E. coli (EHEC), ipaH for enteroinvasive E. coli (EIEC) and aggR for enteroaggregative E. coli (EAEC). In addition, antimicrobial susceptibility profile to 22 antimicrobial agents was also performed by disk diffusion method, and we searched for extended-spectrum beta-lactamases (ESBL) and/or carbapenemase- producing isolates. Isolates that were positive for ESBL and carbapenemase were further investigated for the presence of the genes: blaTEM, blaSHV, blaOXA, blaCTX-M, for ESBL and blaOXA-48 for carbapenemase. Further, isolates had their ability to form biofilms investigated by the red Congo agar method. Virulence genes of E. coli were identified in 21.37% of the tested isolates, which were classified as EPEC (the most prevalent pathotype) and ETEC or EAEC. Ten (8.55%) of the total studied E. coli isolates revealed a multidrug-resistant profile, since they were resistant to three or more antimicrobial classes; whereas four isolates (3.42%) were classified as ESBL-producers and showed the presence of blaTEM gene. None of the isolates exhibited carbapenemase activity nor did they carry carbapenemase genes. From the total of E. coli isolates, 79 (67.52%) were considered potential biofilm producers. These results address a serious public health issue, since artisanal cheeses pose a risk to consumers’ health, since may be sources of dissemination of diarrheogenic E. coli, that can cause from subclinical to severe and fatal infections in children and adults, and also emphasize the need to improve adaptations/adjustments in the manufacturing processes of these products.

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Fluoroquinolone-resistant and extended-spectrum β-lactamase-producing Escherichia coli from the milk of cows with clinical mastitis in Southern Taiwan

Abstract: Lacking virulence genes examined, genetic diverse E. coli isolates are unrelated to human pathogenic E. coli. Enhancing sanitation in milk processing and transportation is needed to eliminate multidrug-resistant (MDR), fluoroquinolone-resistant, and ESBL-producing E. coli isolates.

Cited by 60 publications

References 49 publications

Antimicrobial Resistance inEscherichia coli

Antimicrobial Resistance inEscherichia coli

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

Detection of virulence genes and antimicrobial resistance profiles of Escherichia coli isolates from raw milk and artisanal cheese in Southern Brazil

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