Fluoroquinolone-resistant Vibrio cholerae isolated during a cholera outbreak in India

Krishna, Bhuvana; Patil, Ajinkya; Chandrasekhar, M. R.

doi:10.1016/j.trstmh.2005.07.007

Cited by 53 publications

(34 citation statements)

References 5 publications

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“…Analysis of the gyrA and parC (VC1258 and VC2430, respectively, in Table S3) genes revealed two point mutations in the Haitian strains, i.e., a ser83ile substitution in gyrA and a ser85leu substitution in parC. Both point mutations are associated with quinolone resistance in clinical V. cholerae and have been reported in India and most recently in Nigeria and Cameroon (33)(34)(35)(36)(37). We observed the same point mutations in gyrA and parC in recent isolates from Zimbabwe (CP1038), Thailand (CP1042), and Bangladesh (CP1048).…”

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confidence: 95%

Genomic diversity of 2010 Haitian cholera outbreak strains

Hasan

Choi

Eppinger

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

173

212

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The millions of deaths from cholera during the past 200 y, coupled with the morbidity and mortality of cholera in Haiti since October 2010, are grim reminders that Vibrio cholerae , the etiologic agent of cholera, remains a scourge. We report the isolation of both V . cholerae O1 and non-O1/O139 early in the Haiti cholera epidemic from samples collected from victims in 18 towns across eight Arrondissements of Haiti. The results showed two distinct populations of V. cholerae coexisted in Haiti early in the epidemic. As non-O1/O139 V . cholerae was the sole pathogen isolated from 21% of the clinical specimens, its role in this epidemic, either alone or in concert with V . cholerae O1, cannot be dismissed. A genomic approach was used to examine similarities and differences among the Haitian V . cholerae O1 and V . cholerae non-O1/O139 strains. A total of 47 V . cholerae O1 and 29 V . cholerae non-O1/O139 isolates from patients and the environment were sequenced. Comparative genome analyses of the 76 genomes and eight reference strains of V . cholerae isolated in concurrent epidemics outside Haiti and 27 V . cholerae genomes available in the public database demonstrated substantial diversity of V. cholerae and ongoing flux within its genome.

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confidence: 95%

Genomic diversity of 2010 Haitian cholera outbreak strains

Hasan

Choi

Eppinger

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

173

212

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“…Multi-drug resistant V.cholerae were reported from Rayagada during 2007 (7).Tetracycline resistant V.cholerae were reported from Rayagada district of Odisha during 2010 which were sensitive earlier (8). Fluoroquinolone resistant V.cholerae were reported from south India during 2002 (10).…”

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confidence: 99%

Epidemiology and Antibiogram Profile of <i>Vibrio cholerae</i> Isolates between 2004–2013 from Odisha, India

2018

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A c c e p t e d M a n u s c r i p t 2 SUMMARY: Cholera is an acute diarrhoeal disease caused by Vibrio cholerae O1 and O139 serogroups which are known to cause epidemics of cholera in Odisha. The present study has been envisaged to document the antibiotic resistance pattern among clinical isolates of both serogroups of V.cholerae O1 and O139 isolated during 2004-2013. Nine hundred nine isolates of V.cholerae were included in this study which were identified by standard procedures. Antibiotic sensitivity test was performed by disc diffusion method. The seasonality of cholera in this region indicated that there was one peak observed in the rainy season only. The cholera cases started increasing from the month of July and declined from the month of October onwards. The adult age group of patients were worst affected among all age group of patients. The two different serogroups of V.cholerae O1 and O139 showed different percentage of resistance to all the antibiotics in each year. The serogroup O1 showed uniform high resistance to co-trimoxazole, furazolidone, nalidixic acid throughout the study. Chloramphenicol showed resistance only during 2009, whereas it was sensitive for rest of the years. The emergence of multiple drug resistant V.cholerae may significantly influence the control of future outbreaks and epidemics of cholera in this region.

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“…V. cholerae strains resistant to tetracycline, ampicillin, kanamycin, streptomycin, sulphonamides, trimethoprim, and gentamicin have been previously reported from India and neighboring countries (28,31,32). Multi-drug resistance among V. cholerae strains was identified in our study as well, which limits the therapeutic potential of many drugs (33,34). Ongoing surveillance with regard to the antibiotic sensitivity of diarrhea-causing organisms, as well as prescription practice of physicians, is therefore of paramount importance, which could help the development of appropriate management guidelines at regular intervals.…”

Section: Discussionmentioning

confidence: 65%

Outbreak of Cholera Caused by <i>Vibrio cholerae</i> O1 El Tor Variant Strain in Bihar, India

et al. 2014

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SUMMARY: An outbreak of cholera struck Bihar, an Indian state, in August 2008 following a massive flood. Here we report the phenotypic and genotypic characteristics of Vibrio cholerae strains isolated from patients with diarrhea. Rectal swabs were obtained from patients with diarrhea who were admitted to medical camps or the hospital, and the strains were biochemically and serologically characterized. V. cholerae was isolated from 21 (65.6z) of 32 rectal swabs. Serological studies revealed that all the 21 isolates belonged to V. cholerae O1 Ogawa. Mismatch amplification mutation assay (MAMA)-PCR showed that the isolates belonged to El Tor variant group, and pulsed-field gel electrophoresis (PFGE) proved that these isolates were of a different lineage than the conventional El Tor variant strains. These isolates were resistant to several drugs, including ampicillin, streptomycin, tetracycline, nalidixic acid, and furazolidone. The uniqueness of the current report arises from the fact that records of cholera in Bihar are availiable for the early 1960s but not for the next 4 decades. Moreover, the present study is the first to report a cholera outbreak in Bihar that was caused by an El Tor variant strain.

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Fluoroquinolone-resistant Vibrio cholerae isolated during a cholera outbreak in India

Cited by 53 publications

References 5 publications

Genomic diversity of 2010 Haitian cholera outbreak strains

Genomic diversity of 2010 Haitian cholera outbreak strains

Epidemiology and Antibiogram Profile of <i>Vibrio cholerae</i> Isolates between 2004–2013 from Odisha, India

Outbreak of Cholera Caused by <i>Vibrio cholerae</i> O1 El Tor Variant Strain in Bihar, India

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