Fluorosensing of water stress in plants: Diurnal changes of the mean lifetime and yield of chlorophyll fluorescence, measured simultaneously and at distance with a τ-LIDAR and a modified PAM-fluorimeter, in maize, sugar beet, and kalanchoë

Cerović, Zoran G.; Goulas, Y.; Gorbunov, Maxim Y.; Briantais, Jean-Marie; Camenen, L.; Moya, I.

doi:10.1016/s0034-4257(96)00076-4

Cited by 79 publications

(55 citation statements)

References 28 publications

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“…These findings suggest a future approach to significantly improve the performance of current LUE models, by combining these two optical signals. NPQ and chlorophyll fluorescence emission are considered as direct indicators of photosynthetic activities and physiological stress when plants are under sub-optimal environmental conditions [28,53,59,80,[90][91][92][93] such as low water availability [81,[93][94][95], unfavorable temperatures [96,97], nutrients [98][99][100][101], and salinity [102,103]. Consequently, many studies have utilized the spectral indices, PRI and SIF retrievals, as indicators of stress and down-regulation of LUE and GPP [29,50,51,65,82,92,[104][105][106][107].…”

Section: Discussionmentioning

confidence: 99%

Integrating Solar Induced Fluorescence and the Photochemical Reflectance Index for Estimating Gross Primary Production in a Cornfield

et al. 2013

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Abstract:The utilization of remotely sensed observations for light use efficiency (LUE) and tower-based gross primary production (GPP) estimates was studied in a USDA cornfield. Nadir hyperspectral reflectance measurements were acquired at canopy level during a collaborative field campaign conducted in four growing seasons. The Photochemical Reflectance Index (PRI) and solar induced chlorophyll fluorescence (SIF), were derived. SIF retrievals were accomplished in the two telluric atmospheric oxygen OPEN ACCESSRemote Sens. 2013, 5 6858 absorption features centered at 688 nm (O 2 -B) and 760 nm (O 2 -A). The PRI and SIF were examined in conjunction with GPP and LUE determined by flux tower-based measurements. All of these fluxes, environmental variables, and the PRI and SIF exhibited diurnal as well as day-to-day dynamics across the four growing seasons. Consistent with previous studies, the PRI was shown to be related to LUE (r 2 = 0.54 with a logarithm fit), but the relationship varied each year. By combining the PRI and SIF in a linear regression model, stronger performances for GPP estimation were obtained. The strongest relationship (r 2 = 0.80, RMSE = 0.186 mg CO 2 /m 2 /s) was achieved when using the PRI and SIF retrievals at 688 nm. Cross-validation approaches were utilized to demonstrate the robustness and consistency of the performance. This study highlights a GPP retrieval method based entirely on hyperspectral remote sensing observations.

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Section: Discussionmentioning

confidence: 99%

Integrating Solar Induced Fluorescence and the Photochemical Reflectance Index for Estimating Gross Primary Production in a Cornfield

et al. 2013

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“…It is well documented that a plant under constraint is often characterized by a lower light threshold for the apparition of the NPQ. As a result, a lower Fs is observed for a given light level (Cerovic et al, 1996;Flexas et al, 2000;Günther et al, 1994). However in the present case, the change observed during the campaign concerns mainly the long-term quenching associated with cold acclimation.…”

Section: Evolution Of the Signals During Spring Recoverymentioning

confidence: 99%

Remote sensing of sunlight-induced chlorophyll fluorescence and reflectance of Scots pine in the boreal forest during spring recovery

Louis

Ounis

Ducruet

et al. 2005

Remote Sensing of Environment

102

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A measurement campaign to assess the feasibility of remote sensing of sunlight-induced chlorophyll fluorescence (ChlF) from a coniferous canopy was conducted in a boreal forest study site (Finland). A Passive Multi-wavelength Fluorescence Detector (PMFD) sensor, developed in the LURE laboratory, was used to obtain simultaneous measurements of ChlF in the oxygen absorption bands, at 687 and 760 nm, and a reflectance index, the PRI (Physiological Reflectance Index), for a month during spring recovery. When these data were compared with active fluorescence measurements performed on needles they revealed the same trend. During sunny days fluorescence and reflectance signals were found to be strongly influenced by shadows associated with the canopy structure. Moreover, chlorophyll fluorescence variations induced by rapid light changes (due to transient cloud shadows) were found to respond more quickly and with larger amplitude under summer conditions compared to those obtained under cold acclimation conditions. In addition, ChlF at 760 nm was observed to increase with the chlorophyll content. During this campaign, the CO 2 assimilation was measured at the forest canopy level and was found remarkably well correlated with the PRI index. D

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“…Establishment of NPQ in vivo involves the building of quenching species within the pigment bed, leading to a transition in the overall average lifetime of fluorescence emission from 2.3 to 0.5 ns (4,(14)(15)(16); this is accomplished by an amplitude decrease of the 1.6-1.8 ns Chl a fluorescence component versus that of 0.4-0.5 ns (17). Formation of quenching molecular species has been proposed to involve either the direct de-excitation of 1 Chl* by Cars or the structural reorganization of Lhc proteins into a quenching state.…”

mentioning

confidence: 99%

Time-Resolved Fluorescence Analysis of the Photosystem II Antenna Proteins in Detergent Micelles and Liposomes

et al. 2001

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We have studied the time-resolved fluorescence properties of the light-harvesting complexes (Lhc) of photosystem II (Lhcb) in order to obtain information on the mechanism of energy dissipation (non-photochemical quenching) which is correlated to the conversion of violaxanthin to zeaxanthin in excess light conditions. The chlorophyll fluorescence decay of Lhcb proteins LHCII, CP29, CP26, and CP24 in detergent solution is mostly determined by two lifetime components of 1.2-1.5 and 3.6-4 ns while the contribution of the faster component is higher in CP29, CP26, and CP24 with respect to LHCII. The xanthophyll composition of Lhc proteins affects the ratio of the lifetime components: when zeaxanthin is bound into the site L2 of LHCII, the relative amplitude of the faster component is increased and, consequently, the chlorophyll fluorescence quenching is enhanced. Analysis of quenching in mutants of Arabidopsis thaliana, which incorporate either violaxanthin or zeaxanthin in their Lhc proteins, shows that the extent of quenching is enhanced in the presence of zeaxanthin. The origin of the two fluorescence lifetimes was analyzed by their temperature dependence: since lifetime heterogeneity was not affected by cooling to 77 K, it is concluded that each lifetime component corresponds to a distinct conformation of the Lhc proteins. Upon incorporation of Lhc proteins into liposomes, a quenching of chlorophyll fluorescence was observed due to shortening of all their lifetime components: this indicates that the equilibrium between the two conformations of Lhcb proteins is displaced toward the quenched conformation in lipid membranes or thylakoids with respect to detergent solution. By increasing the protein density in the liposomes, and therefore the probability of protein-protein interactions, a further decrease of fluorescence lifetimes takes place down to values typical of quenched leaves. We conclude that at least two major factors determine the quenching of chlorophyll fluorescence in Lhcb proteins, i.e., intrasubunit conformational change and intersubunit interactions within the lipid membranes, and that these processes are both important in the photoprotection mechanism of nonphotochemical quenching in vivo.The photosynthetic apparatus of higher plants is composed of two photosystems (PS), 1 PSI and PSII, each consisting of a core complex moiety, binding chlorophyll (Chl) a and -carotene, and a peripheral antenna system, binding Chl a, Chl b, and xanthophylls (Cars). The major component of the PSII antenna system is LHCII, a heterotrimer of the Lhcb1, -2, and -3 gene products (also called LHCIIb) binding half of the thylakoid pigments. Minor components are CP29 (LHCIIa), CP26 (LHCIIc), and CP24 (LHCIId), respectively the products of the Lhcb4, -5, and -6 genes, which are monomeric and connect the PSII core complex to the peripheral LHCII trimers.The large absorption cross-section of the antenna systems allows plants to grow in dim light. However, the light intensity experienced by a plant varies largely over ...

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Fluorosensing of water stress in plants: Diurnal changes of the mean lifetime and yield of chlorophyll fluorescence, measured simultaneously and at distance with a τ-LIDAR and a modified PAM-fluorimeter, in maize, sugar beet, and kalanchoë

Cited by 79 publications

References 28 publications

Integrating Solar Induced Fluorescence and the Photochemical Reflectance Index for Estimating Gross Primary Production in a Cornfield

Integrating Solar Induced Fluorescence and the Photochemical Reflectance Index for Estimating Gross Primary Production in a Cornfield

Remote sensing of sunlight-induced chlorophyll fluorescence and reflectance of Scots pine in the boreal forest during spring recovery

Time-Resolved Fluorescence Analysis of the Photosystem II Antenna Proteins in Detergent Micelles and Liposomes

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