Fluorous derivatization and fluorous-phase separation for fluorometric determination of naproxen and felbinac in human plasma

Challenges in the simultaneous quantitation of sumatriptan and naproxen in human plasma: Application to a bioequivalence study

“…Several methods have used either liquid-liquid extraction (LLE) [9,11,21] or solid phase extraction (SPE) [13,[16][17][18][19]22] for the extraction of SUM (as a single analyte) from human plasma. However, protein precipitation (PP) has been successfully established for sample clean-up of NAP from human plasma [32,47,48]. Berges et al [49] have reported a separate SPE procedure for extraction of SUM and a protein precipitation protocol with acetonitrile for NAP for their simultaneous quantification from human plasma.…”

“…A critical comparison of different analytical methods for identification and determination of several triptans including SUM has been reviewed by Saka [23]. Like SUM, there are numerous methods for the determination of NAP in biological fluids by a variety of analytical techniques like capillary electrophoresis [24], flow injection chemiluminescence [25], room temperature liquid phosphorimetry [26], electrokinetic capillary chromatography [27], fluorescence spectrometry [28][29][30][31][32], GC-MS [33], HPLC with chemiluminescence [34], fluorescence [33,[35][36][37][38] and UV detection [33,36,[39][40][41][42][43][44][45][46] and LC-MS/MS [46][47][48]. NAP has been determined in human plasma and dried blood spots by LC-MS/MS for pharmacokinetic comparison in the concentration range of 0.5-100 g/mL [47].…”

Selective and sensitive liquid chromatographic determination method of 5-hydroxyindoles with fluorous and fluorogenic derivatization

“…Recently, we have also developed LC analytical methods for native fluorescent compounds based on "fluorous derivatization," in which "fluorous" refers to the unique affinity between highly fluorinated compounds [29][30][31]. The principle of this method is that native fluorescent analytes are derivatized precolumn by attachment of a non-fluorescent fluorous compound to a particular functional group; thereafter, the fluorous-derivatized analytes are extremely retained on a fluorous phase LC column, thus achieving selective separation of analytes from biological sample matrices.…”

“…Neither non-fluorescent compounds nor underivatized native fluorescent compounds interfere with the detection. This method was successfully applied to the determination of pharmaceutical agents possessing carboxylic groups in the human plasma and urine after administration [30], as well as native fluorescent biogenic amines in the human urine [31].…”

“…Spiked concentrations (pmol/mL plasma): 5-HTRP (150), other 5-HIs (30). d Relative standard deviation obtained with peak area for 30 pmol/mL (5-HT, 5-HIAA, NAS, and 5-HTOL) and 150 pmol/mL (5-HTRP) spiked plasma.…”