Fluorous Phase‐Directed Peptide Assembly Affords Nano‐Peptisomes Capable of Ultrasound‐Triggered Cellular Delivery

Abstract

Here, we report the design, synthesis and efficacy of a new class of ultrasound (US)-sensitive self-assembled peptide-based nanoparticle. Peptisomes are prepared via templated assembly of a de novo designed peptide at the interface of fluorinated nanodroplets. Utilizing peptide assembly allows for facile particle synthesis, direct incorporation of bioactive sequences displayed from the particle corona, and the ability to easily encapsulate biologics during particle preparation using a mild solvent exchange procedure. Further, nano-peptisome size can be precisely controlled by simply modulating the starting peptide and fluorinated solvent concentrations during synthesis. Biomolecular cargo encapsulated within the particle core can be directly delivered to the cytoplasm of cells upon US-mediated rupture of the carrier. Thus, nano-peptisomes represent a novel class of US-activated carriers that can shuttle cell-impermeable biomacromolecules into cells with spatial and temporal precision.

Controlling nanoemulsion surface chemistry with poly(2-oxazoline) amphiphiles

“…90 Recently, volatile per-uorocarbon droplets have been effectively stabilized by Gianneschi and coworkers through triblock poly(norbornene)s, 45 and by Medina et al through crosslinked peptides. 44 However, neither of these efforts explored surface modication. Taken together, these works suggest interest and need for custom polymer surfactants that facilitate functionalizable, stable PFC nanoemulsions.…”

“…Simple surfactants such as phospholipids and poloxamers ( Fig. 1B) are routinely used for industrial applications, while recently engineered peptide, [42][43][44] polymer, 45 and nanoparticle 46 surfactants have produced responsive materials 47,48 and sophisticated architectures. Slight changes in surfactant structure can drastically affect the physiochemical properties of the emulsions.…”

Ultrasound‐Activated Sensitizers and Applications

“…Copyright 2018, American Chemical Society. ( d ‐G) Reproduced with permission . Copyright 2017, Wiley‐VCH.…”

Biodegradable cell‐laden starch foams for the rapid fabrication of 3D tissue constructs and the application in neural tissue engineering

“…To observe the morphology and distribution of RSC96 Schwann cells embedded in the starch foams, cryosections of the cell‐laden foams were stained with phalloidin, which can bind to intracellular filamentous actin to labeled cytoskeleton . As shown in Figure A, after 1 day, RSC96 Schwann cells uniformly embedded in the foams did not completely spread and form extensions.…”