Fluoxetine induces photochemistry-derived oxidative stress on Ulva lactuca

Feijão, Eduardo; Carvalho, Ricardo Cruz de; Duarte, Irina A.; Matos, Ana Rita; Cabrita, Maria Teresa; Utkin, Andrei B.; Caçador, Isabel; Marques, João; Novais, Sara C.; Lemos, Marivane; Patrick, Ruth; Fonseca, Vanessa F.; Duarte, Bernardo

doi:10.3389/fenvs.2022.963537

Cited by 3 publications

(2 citation statements)

References 96 publications

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“…To assess enzymatic activity, five samples per group were taken and a quantity of 200 mg of fresh weight was ground with liquid nitrogen using a mortar and pestle to obtain a homogenized sample. the soluble protein fraction was extracted according to Feijão et al (2022) and centrifuging the samples at 12,000 × g for 15 minutes at 4°C to recover the supernatant. The measurements were carried out at 25°C using an Epoch 2 Microplate Reader (BioTek Instruments, VA, United States).…”

Section: Hydrogen Peroxide Content and Antioxidant Enzymes Activitymentioning

confidence: 99%

The use of chitosan oligosaccharide to improve artemisinin yield in well-watered and drought-stressed plants

et al. 2023

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IntroductionArtemisinin is a secondary metabolite well-known for its use in the treatment of malaria. It also displays other antimicrobial activities which further increase its interest. At present, Artemisia annua is the sole commercial source of the substance, and its production is limited, leading to a global deficit in supply. Furthermore, the cultivation of A. annua is being threatened by climate change. Specifically, drought stress is a major concern for plant development and productivity, but, on the other hand, moderate stress levels can elicit the production of secondary metabolites, with a putative synergistic interaction with elicitors such as chitosan oligosaccharides (COS). Therefore, the development of strategies to increase yield has prompted much interest. With this aim, the effects on artemisinin production under drought stress and treatment with COS, as well as physiological changes in A. annua plants are presented in this study.MethodsPlants were separated into two groups, well-watered (WW) and drought-stressed (DS) plants, and in each group, four concentrations of COS were applied (0, 50,100 and 200 mg•L-1). Afterwards, water stress was imposed by withholding irrigation for 9 days.ResultsTherefore, when A. annua was well watered, COS did not improve plant growth, and the upregulation of antioxidant enzymes hindered the production of artemisinin. On the other hand, during drought stress, COS treatment did not alleviate the decline in growth at any concentration tested. However, higher doses improved the water status since leaf water potential (YL) improved by 50.64% and relative water content (RWC) by 33.84% compared to DS plants without COS treatment. Moreover, the combination of COS and drought stress caused damage to the plant’s antioxidant enzyme defence, particularly APX and GR, and reduced the amount of phenols and flavonoids. This resulted in increased ROS production and enhanced artemisinin content by 34.40% in DS plants treated with 200 mg•L-1 COS, compared to control plants.ConclusionThese findings underscore the critical role of ROS in artemisinin biosynthesis and suggest that COS treatment may boost artemisinin yield in crop production, even under drought conditions.

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Section: Hydrogen Peroxide Content and Antioxidant Enzymes Activitymentioning

confidence: 99%

The use of chitosan oligosaccharide to improve artemisinin yield in well-watered and drought-stressed plants

et al. 2023

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“…Oxidative stress ratio = SOD Activity APX Activity + CAT Activity (3) Lipid peroxidation was determined according to [66] with proper amendments [26,67]. Absorbance was registered at 532 and 600 nm and TBARS concentrations were determined through the molar extinction coefficient, 155 mM −1 cm −1 [66] and expressed as mM of malondialdehyde (MDA) cell −1 (P. tricornutum), or mM MDA g −1 FW (U. lactuca).…”

Section: Oxidative Stressmentioning

confidence: 99%

Ecotoxicological Effects of the Anionic Surfactant Sodium Dodecyl Sulfate (SDS) in Two Marine Primary Producers: Phaeodactylum tricornutum and Ulva lactuca

Carvalho

Feijão

Matos

et al. 2022

Toxics

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Sodium Dodecyl Sulfate (SDS) is an anionic surfactant, extensively used in detergents, household and personal care products, as well as in industrial processes. The present study aimed to disclose the potential toxicological effects of SDS exposure under environmentally relevant concentrations (0, 0.1, 1, 3, and 10 mg L−1) on the physiology and biochemistry (photosynthesis, pigment, and lipid composition, antioxidative systems, and energy balance) of two marine autotrophs: the diatom Phaeodactylum tricornutum and the macroalgae Ulva lactuca. A growth rate (GR) reduction in P. tricornutum was observed with a classic dose-response effect towards the highest applied concentration, while a GR increase occurred in U. lactuca. Regarding photochemistry, the decrease in the fluorescence of the OJIP curves and laser-induced fluorescence allowed a better separation between SDS treatments in U. lactuca compared with P. tricornutum. Although all pigments significantly decreased in U. lactuca at the highest concentrations (except for antheraxanthin), no significant variations occurred in P. tricornutum. On the other hand, changes in fatty acid content were observed in P. tricornutum but not in U. lactuca. In terms of classical biomarker assessment, a dose-effect relationship of individual biomarkers versus SDS dose applied; U. lactuca displayed a higher number of biomarker candidates, including those in distinct metabolic pathways, increasing its usefulness for ecotoxicological applications. By evaluating the potential application of optical and biochemical traits, it was evident that the fatty acid profiles of the different exposure groups are excellent candidates in P. tricornutum, concomitant with the characteristics of this anionic surfactant. On the other hand, the results presented by laser-induced fluorescence and some parameters of PAM fluorometry in U. lactuca may be an advantage in the field, offering non-invasive, fast, easy-to-use, high-throughput screening techniques as excellent tools for ecotoxicology assessment.

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Assessing pharmaceuticals in the green seaweed Ulva lactuca through a multi-residue UHPLC-ToF-MS strategy

Leston

Rosa

Pouca

et al. 2023

Marine Pollution Bulletin

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Fluoxetine induces photochemistry-derived oxidative stress on Ulva lactuca

Cited by 3 publications

References 96 publications

The use of chitosan oligosaccharide to improve artemisinin yield in well-watered and drought-stressed plants

The use of chitosan oligosaccharide to improve artemisinin yield in well-watered and drought-stressed plants

Ecotoxicological Effects of the Anionic Surfactant Sodium Dodecyl Sulfate (SDS) in Two Marine Primary Producers: Phaeodactylum tricornutum and Ulva lactuca

Assessing pharmaceuticals in the green seaweed Ulva lactuca through a multi-residue UHPLC-ToF-MS strategy

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