In the nearly two decades of Cassini flight, adaptations to navigation processes were applied as characteristics unique to Cassini were identified and spacecraft configuration changes were implemented. Additionally, operational experience was leveraged into more efficient processes, better modeling, and more robust contingency strategies. Trajectory adjustments were implemented to allow further investigation of surprising science discoveries meriting more attention. Unique analyses were performed to determine how to best accomplish atypical science observations while maintaining acceptable risk levels. Descriptions of the most significant adaptations are organized according to the affected navigation subsystems: trajectory design, orbit determination, optical navigation, and maneuver design.