The purpose of the present work is to evaluate the flying qualities of the Unmanned Airplane for Ecological Conservation as a remote piloted vehicle via flight simulation. The study includes the evaluation of the interface and the display between the operator and the simulator. The model of the aircraft was simulated using FlightGear. Aerodynamic, geometric, thrust and mass characteristics of the airplane were introduced to the simulator by a code written in C++. Virtual flight trials were performed and the flying qualities were obtained through a "Briefing Guide and Rating Information for Simulated Handling Qualities Experiments Adapted for Remotely Piloted Vehicles." It could be concluded that the Unmanned Airplane for Ecological Conservation has excellent flying qualities, rating the aircraft in Level 1. In addition, the interface with the operators was highly efficient because the display information contributes satisfactorily with the decision-making, rating the display as Level 1 on the Modified Cooper and Harper Evaluation Tool for Unmanned Vehicles Displays. The simulator is recommended to simulate flying qualities evaluation of unmanned aerial vehicles.