FMRFa receptor stimulated Ca<sup>2+</sup>signals alter the activity of flight modulating central dopaminergic neurons in<i>Drosophila melanogaster</i>

Ravi, Preethi; Trivedi, Deepti; Hasan, Gaiti

doi:10.1101/336768

Cited by 3 publications

(1 citation statement)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To study Ca 2+ dynamics in dopaminergic neurons we used KCl, a depolarizing stimulus known to raise the resting membrane potential and open voltage‐gated Ca 2+ channels on the plasma membrane (Ravi, Trivedi, and Hasan 2018); 50 frames of basal activity were recorded, followed by a manual addition of KCl to achieve a final concentration of 70 mM and 300 frames were recorded afterwards. Ca 2+ dynamics were analysed and quantified following (Ravi et al, 2018) description.…”

Section: Methodsmentioning

confidence: 99%

Plasma membrane calcium ATPase downregulation in dopaminergic neurons alters cellular physiology and motor behaviour in Drosophila melanogaster

Erhardt

Marcora

Frenkel

et al. 2021

Eur J of Neuroscience

View full text Add to dashboard Cite

The accumulation of Ca2+ and its subsequent increase in oxidative stress is proposed to be involved in selective dysfunctionality of dopaminergic neurons, the main cell type affected in Parkinson's disease. To test the in vivo impact of Ca2+ increment in dopaminergic neurons physiology, we downregulated the plasma membrane Ca2+ ATPase (PMCA), a pump that extrudes cytosolic Ca2+, by expressing PMCARNAi in Drosophila melanogaster dopaminergic neurons. In these animals, we observed major locomotor alterations paralleled to higher cytosolic Ca2+ and increased levels of oxidative stress in mitochondria. Interestingly, although no overt degeneration of dopaminergic neurons was observed, evidences of neuronal dysfunctionality were detected such as increases in presynaptic vesicles in dopaminergic neurons and in the levels of dopamine in the brain, as well as presence of toxic effects when PMCA was downregulated in the eye. Moreover, reduced PMCA levels were found in a Drosophila model of Parkinson's disease, Parkin knock‐out, expanding the functional relevance of PMCA reduction to other Parkinson's disease‐related models. In all, we have generated a new model to study motor abnormalities caused by increments in Ca2+ that lead to augmented oxidative stress in a dopaminergic environment, added to a rise in synaptic vesicles and dopamine levels.

show abstract

Section: Methodsmentioning

confidence: 99%

Plasma membrane calcium ATPase downregulation in dopaminergic neurons alters cellular physiology and motor behaviour in Drosophila melanogaster

Erhardt

Marcora

Frenkel

et al. 2021

Eur J of Neuroscience

View full text Add to dashboard Cite

show abstract

Extended Flight Bouts Require Disinhibition from GABAergic Mushroom Body Neurons

et al. 2018

Self Cite

View full text Add to dashboard Cite

Insect flight is a complex behavior that requires the integration of multiple sensory inputs with flight motor output. Although previous genetic studies identified central brain monoaminergic neurons that modulate Drosophila flight, neuro-modulatory circuits underlying sustained flight bouts remain unexplored. Certain classes of dopaminergic and octopaminergic neurons that project to the mushroom body, a higher integrating center in the insect brain, are known to modify neuronal output based on contextual cues and thereby organismal behavior. This study focuses on how monoaminergic modulation of mushroom body GABAergic output neurons (MBONs) regulates the duration of flight bouts. Octopaminergic neurons in the sub-esophageal zone stimulate central dopaminergic neurons (protocerebral anterior medial, PAM) that project to GABAergic MBONs. Either inhibition of octopaminergic and dopaminergic neurons or activation of GABAergic MBONs reduces the duration of flight bouts. Moreover, activity in the PAM neurons inhibits the GABAergic MBONs. Our data suggest that disinhibition of the identified neural circuit very likely occurs after flight initiation and is required to maintain the ''flight state'' when searching for distant sites, possibly related to food sources, mating partners, or a suitable egg-laying site.

show abstract

Genome-Wide Identification of Neuropeptides and Their Receptors in an Aphid Endoparasitoid Wasp, Aphidius gifuensi

Xue

Gao

et al. 2021

Insects

View full text Add to dashboard Cite

In insects, neuropeptides and their receptors not only play a critical role in insect physiology and behavior but also are the potential targets for novel pesticide discoveries. Aphidius gifuensis is one of the most important and widespread aphid parasitoids, and has been successfully used to control aphid. In the present work, we systematically identified neuropeptides and their receptors from the genome and head transcriptome of A. gifuensis. A total of 35 neuropeptide precursors and 49 corresponding receptors were identified. The phylogenetic analyses demonstrated that 35 of these receptors belong to family-A, four belong to family-B, two belong to leucine-rich repeat-containing GPCRs, four belong to receptor guanylyl cyclases, and four belong to receptor tyrosine kinases. Oral ingestion of imidacloprid significantly up-regulated five neuropeptide precursors and four receptors whereas three neuropeptide precursors and eight receptors were significantly down-regulated, which indicated that these neuropeptides and their receptors are potential targets of some commercial insecticides. The RT-qPCR results showed that dopamine receptor 1, dopamine receptor 2, octopamine receptor, allatostatin-A receptor, neuropeptides capa receptor, SIFamide receptor, FMRFamide receptor, tyramine receptor and short neuropeptide F predominantly were expressed in the head whilst the expression of ion transport peptide showed widespread distribution in various tissues. The high expression levels of these genes suggest their important roles in the central nervous system. Taken together, our study provides fundamental information that may further our understanding of neuropeptidergic signaling systems in the regulation of the physiology and behavior of solitary wasps. Furthermore, this information could also aid in the design and discovery of specific and environment-friendly insecticides.

show abstract

FMRFa receptor stimulated Ca²⁺signals alter the activity of flight modulating central dopaminergic neurons inDrosophila melanogaster

Abstract: 22Neuropeptide signaling influences animal behavior by modulating neuronal was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.

Cited by 3 publications

References 70 publications

Plasma membrane calcium ATPase downregulation in dopaminergic neurons alters cellular physiology and motor behaviour in Drosophila melanogaster

Plasma membrane calcium ATPase downregulation in dopaminergic neurons alters cellular physiology and motor behaviour in Drosophila melanogaster

Extended Flight Bouts Require Disinhibition from GABAergic Mushroom Body Neurons

Genome-Wide Identification of Neuropeptides and Their Receptors in an Aphid Endoparasitoid Wasp, Aphidius gifuensi

Contact Info

Product

Resources

About

FMRFa receptor stimulated Ca2+signals alter the activity of flight modulating central dopaminergic neurons inDrosophila melanogaster

Abstract: 22Neuropeptide signaling influences animal behavior by modulating neuronal was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.

Cited by 3 publications

References 70 publications

Plasma membrane calcium ATPase downregulation in dopaminergic neurons alters cellular physiology and motor behaviour in Drosophila melanogaster

Plasma membrane calcium ATPase downregulation in dopaminergic neurons alters cellular physiology and motor behaviour in Drosophila melanogaster

Extended Flight Bouts Require Disinhibition from GABAergic Mushroom Body Neurons

Genome-Wide Identification of Neuropeptides and Their Receptors in an Aphid Endoparasitoid Wasp, Aphidius gifuensi

Contact Info

Product

Resources

About

FMRFa receptor stimulated Ca²⁺signals alter the activity of flight modulating central dopaminergic neurons inDrosophila melanogaster