FNDC5/irisin is expressed and regulated differently in human periodontal ligament cells, dental pulp stem cells and osteoblasts

Yang, Yang; Pullisaar, Helen; Landin, Maria A.; Heyward, Catherine Anne; Schröder, Maria; Geng, Tianxiang; Grano, Maria; Reseland, Janne Elin

doi:10.1016/j.archoralbio.2021.105061

Cited by 13 publications

(16 citation statements)

References 40 publications

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“…et al 2021). FNDC5, a transmembrane protein of skeletal muscle, is regulated by peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) during exercise-related activities and release of irisin (Maak et al 2021;Yang et al 2021;Lourenco et al 2019). This process activates mitochondrial energy regulation, fatty acid oxidation, fat browning, glucose metabolism and osteogenesis (Deng et al 2020;Cho et al 2021).…”

Section: Introductionmentioning

confidence: 99%

CircRNA AFF4 induced by KDM1A promotes osteogenic differentiation through FNDC5/Irisin pathway

Liu

Chen

Zhong

et al. 2022

Mol Med

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Background Circular RNA (circ) AFF4 was documented to regulate osteogenesis but the underlying mechanism remains to be elucidated. The preliminary study showed that circ_AFF4 may promote osteogenesis via FNDC5/Irisin. Furthermore, the online prediction tool indicated the interaction of circ_AFF4, insulin-like growth factor-2 mRNA-binding protein 3 (IGF2BP3), FNDC5 and lysine (K)-specific demethylase 1 A (KDM1A). Therefore, this study aims to elucidate the relationships of KDM1A, circ_AFF4, IGF2BP3 and FNDC5/Irisin during osteogenesis. Methods The alkaline phosphatase (ALP) activities and osteogenic-related factors were determined using ALP and alizarin red S (ARS) staining, real-time quantitative PCR(RT-qPCR) and western blot. Immunoprecipitation (RIP), pull-down assay and fluorescence in situ hybridization (FISH) were used to examine the interactions among circ_AFF4/IGF2BP3/FNDC5. A mouse in vivo model was utilized to further confirm the regulatory effect on bone formation. Results Circ_AFF4 and KDM1A expression levels were increased during osteoinduction of BM-MSCs. Knockdown of circ_AFF4 and KDM1A significantly suppressed BM-MSC osteogenesis. We also proved that KDM1A directly bound to circ_AFF4 and FNDC5 promoter and induced circ_AFF4 and FNDC5 expression. Furthermore, circ_AFF4 enhanced the stability of FNDC5 by generating a circ_AFF4, IGF2BP3 and FNDC5 RNA-protein complex, and thereby induced Irisin and osteogenesis. The in vitro data was confirmed with in vivo model. Conclusion These findings elucidate that KDM1A induces circ_AFF4, which promotes promote osteogenesis via IGF2BP3. This study indicates that circ_AFF4 may potentially represent a critical therapeutic target for the diseases.

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Section: Introductionmentioning

confidence: 99%

CircRNA AFF4 induced by KDM1A promotes osteogenic differentiation through FNDC5/Irisin pathway

Liu

Chen

Zhong

et al. 2022

Mol Med

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“…Irisin is also expressed and regulated differently in human periodontal ligament cells, dental pulp stem cells and osteoblasts (13). Other than this, irisin has been found to be involved in cementoblast differentiation as well (14).…”

Section: Introductionmentioning

confidence: 99%

Salivary Irisin and periodontal clinical parameters in patients of chronic periodontitis and healthy individuals: A novel salivary myokine for periodontal disease

et al. 2021

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Objective: To evaluate changes in the levels of salivary irisin in chronic periodontitis, and to correlate the two. Methods: The analytical cross-sectional study was conducted at Fatima Memorial Hospital & College of Dentistry, Lahore, Pakistan, from September 2017 to March 2018, and comprised patients of either gender visiting the periodontic out-patient department. The subjects were divided into group I, which had periodontally healthy controls, and group II, which had an equal number of chronic periodontitis patients. Chronic periodontitis was assessed on basis of pocket probing depth, clinical attachment level, plaque percentage and bleeding on probing. Also, 4ml of un-stimulated saliva was collected for the quantification of irisin protein using enzyme-linked immunosorbent assay. Data was analysed using SPSS 25. Results: Of the 40 subjects, there were 20 (50%) in group I with 10 (50%) males and 10 (50%) females having an overall mean age of 37.60±2.58 years. The remaining 20 (50%) subjects were in group II with 16 (80%) males and 4 (20%) females having an overall mean age of 43.25±6.10 years. Mean salivary irisin level in group II was 6.80±3.97ng/ml compared to 3.99±2.48 ng/ml in group I (p=0.009). Periodontal clinical parameters in both the groups were positively but non-significantly correlated with salivary irisin levels (p>0.05) except for decreased plaque percentage in group I (p<0.05). Conclusion: Salivary irisin levels increased in chronic periodontitis and decreased with decreasing plaque percentage in healthy individuals, indicating that this myokine can act as a biomarker for chronic periodontal disease. Continuous...

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“…This study assessed the expression and regulation of FNDC5/ irisin in oral tissues, and it revealed that FNDC5 was expressed in rodent periodontal ligament (PDL), dental pulp and alveolar bone, as well as in human PDL (hPDL) cells, dental pulp cells (hDPCs) and osteoblasts (hOBs) [13]. Further, it was found that FNDC5 expression was regulated in all the tested cell types, and that odontoblast-like differentiation affected FNDC5/ irisin expression and secretion in hDPCs [13].…”

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confidence: 99%

“…Research indicates that FNDC5/irisin might play a role in vital pulp therapy and potentially contribute to repairing the pulp-dentine complex as odontoblastic differentiation of hDPCs affects FNDC5/irisin expression and secretion [13]. The expression of FNDC5 was gradually enhanced over-time during differentiation.…”

mentioning

confidence: 99%

“…The teeth are constantly under physical stimulation, and PDL is essential in providing tooth mobility and functionality [18]. As FNDC5 expression was found in both hPDL cells and rodent PDL [13], it could be presumed that FNDC5/irisin expression and function in periodontium might be regulated by physical stimulation. The autoregulation of FNDC5 expression was investigated by administration of recombinant human irisin, while dietary influence was tested by administration of all-trans retinoic acid (ATRA).…”

mentioning

confidence: 99%

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Expression and Regulation of FNDC5/irisin in Periodontium and Dental Pulp

2022

AOD

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FNDC5/irisin is expressed and regulated differently in human periodontal ligament cells, dental pulp stem cells and osteoblasts

Cited by 13 publications

References 40 publications

CircRNA AFF4 induced by KDM1A promotes osteogenic differentiation through FNDC5/Irisin pathway

CircRNA AFF4 induced by KDM1A promotes osteogenic differentiation through FNDC5/Irisin pathway

Salivary Irisin and periodontal clinical parameters in patients of chronic periodontitis and healthy individuals: A novel salivary myokine for periodontal disease

Expression and Regulation of FNDC5/irisin in Periodontium and Dental Pulp

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