Foam Control in Fermentation Bioprocess

Etoc, Ariane; Delvigne, Frank; Lecomte, Jean-Paul; Thonart, Philippe

doi:10.1007/978-1-59745-268-7_32

Cited by 6 publications

(3 citation statements)

References 12 publications

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“…Loading volume was 50 mL, air flow 300 mL/min, aerate time 20 s. Foam height (H) was used to characterize the foam ability (unit cm); half-life period, the time it takes the height of foam to decay to half of the H (unit s), was used to characterize the foam stability. [16] Model 1 Polymyxin E, undesired proteins, and its producer cells are all adsorbed directly at gasliquid interface. Model 2 Polymyxin E and undesired proteins are adsorbed at gas-liquid interfaces or adhered by each other, while cells distribute in liquid between bubbles.…”

Section: Part 2: Fermentation Coupling With Foam Separationmentioning

confidence: 99%

Process improvement for fermentation coupling with foam separation: a convenient strategy for cell recycle

Zhang

Dong

et al. 2015

Asia-Pacific J Chem Eng

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Applications of fermentation coupling with foam separation technology show great advantages in many natural biosurfactants fermentation such as nisin, surfactin, and polymyxin E. However, only a few reports focus on process improvement, i.e. cells overflowing with foam. Because the cells also have hydrophobicity, it will overflow during foam separation, which decrease the productivity and contaminate the environment. Therefore, it is important to prevent cell from overflowing. In this study, a new approach was proposed to increase yield, using fermentation coupling with foam separation technology. Meanwhile, we tried to prevent cell from overflowing. This strategy was characterized by enrichment, recovery percentage, and inactivity percentage for product and cells. The results indicated pH was the most important factor in foam separation of polymyxin E. The optimal condition for fermentation coupling with foam separation was determined. The maximum total titer of polymyxin E reached 917 932.31 U (1147.4 U/mL) using coupling strategy that was 1.36 times more than total titer in shaken-flask culture. Additionally, the adsorption mechanism for polymyxin E and cells was studied. The results indicated polymyxin E were mainly adsorbed on bubble, while the cells were partly in liquid form and partly adsorbed on undesired protein. Undesired protein can be considered as a stabilizer in foam separation of polymyxin E.

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Section: Part 2: Fermentation Coupling With Foam Separationmentioning

confidence: 99%

Process improvement for fermentation coupling with foam separation: a convenient strategy for cell recycle

Zhang

Dong

et al. 2015

Asia-Pacific J Chem Eng

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“…When the thereby stabilized gas bubbles reach the reactor headspace, interstitial liquid is entrained by the hydrophilic part of the surfactants between the bubbles, forming the foam lamellae [ 30 , 31 ]. Consequently, the cultivation process is hampered, e.g., by loss of medium and bacterial cells entrapped in the foam, by a reduced oxygen transfer from the headspace into the culture, and a generally increased system heterogeneity [ 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

Uncoupling Foam Fractionation and Foam Adsorption for Enhanced Biosurfactant Synthesis and Recovery

et al. 2020

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The production of biosurfactants is often hampered by excessive foaming in the bioreactor, impacting system scale-up and downstream processing. Foam fractionation was proposed to tackle this challenge by combining in situ product removal with a pre-purification step. In previous studies, foam fractionation was coupled to bioreactor operation, hence it was operated at suboptimal parameters. Here, we use an external fractionation column to decouple biosurfactant production from foam fractionation, enabling continuous surfactant separation, which is especially suited for system scale-up. As a subsequent product recovery step, continuous foam adsorption was integrated into the process. The configuration is evaluated for rhamnolipid (RL) or 3-(3-hydroxyalkanoyloxy)alkanoic acid (HAA, i.e., RL precursor) production by recombinant non-pathogenic Pseudomonas putida KT2440. Surfactant concentrations of 7.5 gRL/L and 2.0 gHAA/L were obtained in the fractionated foam. 4.7 g RLs and 2.8 g HAAs could be separated in the 2-stage recovery process within 36 h from a 2 L culture volume. With a culture volume scale-up to 9 L, 16 g RLs were adsorbed, and the space-time yield (STY) increased by 31% to 0.21 gRL/L·h. We demonstrate a well-performing process design for biosurfactant production and recovery as a contribution to a vital bioeconomy.

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“…Other active principles of antifoams are oils (mineral, vegetable), fatty esters, polyethers, and alcohols. Some commercial antifoams are sold in the form of oil‐in‐water emulsions, because it is easier to carry out the dosing during application …”

Section: Introductionmentioning

confidence: 99%

Characterization of the liquid fractions from textile sludge pyrolysis and their application as defoamers

et al. 2018

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The aim of this study was to investigate the characteristics and the defoamer capacity of bio-oils obtained from textile sludge pyrolysis. The pyrolysis was carried out at two temperatures: 310 and 500 8C, and the bio-oils were analyzed after 7 days and after 2 months of storage under refrigeration. The structure of the bio-oils was determined by Fourier transform infrared spectroscopy (FTIR) and the extraction of polar compounds by solid phase micro extraction (SPME) coupled with gas chromatography/mass spectrometry (GC/MS) analysis. According to the chromatography and FTIR results, aromatic hydrocarbons, amines, silicone, and organic sulphur compounds were present in the pyrolysis oils. The Bikerman test showed that 1 mL of bio-oil obtained from pyrolysis at 500 8C can break down a column of foam in less than 1 min, which is comparable to the results of commercial antifoams. According to these results, these oils can be used as defoamers, even at the textile plant itself.

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Foam Control in Fermentation Bioprocess

Cited by 6 publications

References 12 publications

Process improvement for fermentation coupling with foam separation: a convenient strategy for cell recycle

Process improvement for fermentation coupling with foam separation: a convenient strategy for cell recycle

Uncoupling Foam Fractionation and Foam Adsorption for Enhanced Biosurfactant Synthesis and Recovery

Characterization of the liquid fractions from textile sludge pyrolysis and their application as defoamers

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