Foam fractionation of a recombinant biosurfactant apolipoprotein

Lethcoe, Kyle; Fox, Colin A.; Ryan, Robert O.

doi:10.1016/j.jbiotec.2021.11.004

Cited by 10 publications

(3 citation statements)

References 33 publications

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“…Its widespread offline utilization is due to the purification capacity of intact proteins with mild chromatographic conditions to permit further additional sample preparation steps with minimal impact on the conformational structure. The type of analytes and samples investigated by SEC in recent studies included exosomal proteins in human plasma [30], plasma exosomal proteins of corneal epithelial injury [31], extracellular vesicles in cancer cells [32,33], foam of recombinant biosurfactant apolipoprotein [34], peptides in alcalase hydrolisated pollen [35], circulating microparticles proteins to identify preterm babies before the end of 35 weeks of gestation [36], extracellular vesicle in cord blood of preterm and term births [37], α-Glucosidase-inhibitory peptides in dry-cured ham [38], extracellular vesicle in blood plasma [39], proteins in endogenous membrane proteins [40], N-Glycoproteins and phosphoproteins in human urine [41], high-density lipoproteins from plasma [42], proteins in antivenoms [43] and venoms of snakes [44], artificially created immune complexes on human serum [45], labelled proteins of E. coli lysate [46], extracellular vesicles in serum [47], β-Type Hemocyanin in shrimps [48], and extracellular vesicles in PLB-985 neutrophil-like cells [49].…”

Section: 1mentioning

confidence: 99%

Recent stationary phase‐based fractionation strategies in proteomic analysis

Ribeiro

Urban

2023

J of Separation Science

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In this article, we review stationary phase‐assisted sample prefractionation in proteomic analysis in 2019–2022 period. Applications are grouped according to the mode of retention used in the prefractionation step prior to the final analysis by low‐pH reversed‐phase liquid chromatography‐tandem mass spectrometry. Online or offline instrumental configurations are also commented on, with a highlight on novel online platforms. From the articles reviewed in this period, the order of popularity of chromatographic modes for sample fractionation is affinity chromatography > size exclusion chromatography > hydrophilic interaction chromatography > high‐pH reversed‐phase liquid chromatography > ion exchange chromatography.

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Section: 1mentioning

confidence: 99%

Recent stationary phase‐based fractionation strategies in proteomic analysis

Ribeiro

Urban

2023

J of Separation Science

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“…Reactors and apparatus involving a gas-liquid or gas-liquidsolid phase have been applied to various processes including foam fractionation-based bioseparation [74], enzymatic oxidation reactions [75,76], water treatments [77], and cell cultures [78]. An external loop airlift bubble column (ELBC) is one of the practical and promising apparatus for biochemical engineering processes [75] because of its well-characterized and relatively mild flow conditions and wide scalability [79].…”

Section: Gas-liquid Flow-induced Characteristics Of Liposomesmentioning

confidence: 99%

Gas‐Liquid Flow‐Induced Characteristics of Dispersed Liposome‐Based Particles

Yoshimoto

2023

Chem Eng & Technol

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Liposomes are closed bilayers of phospholipids formed in aqueous media. The hydrodynamic property of colloidal dispersions of liposomes is one of the factors which alter the structure of liposomes. The present review article discusses the mechanistic aspects of the deformation, breakage, and fusion of liposomes in different liquid shear flows. Then, characteristic features reported with respect to the particles consisting of liposomes and bubbles are discussed to explore the role of shear flows induced by the acoustically active bubbles. Furthermore, the gas‐liquid flow‐induced characteristics of liposomes are described for their application to kinetically controllable enzymatic reactions in bubble‐column bioreactors.

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“…During the removal of surfactant micropollutants from a liquid in a bubble column, surfactant molecules are adsorbed on air bubble surfaces, and the generated foam contains a higher amount of the surfactant than the remaining liquid solution . This method is called bubble separation or foam fractionation, which is used in protein purifications, − pharmaceutical and micropollutant removals, − and wastewater treatments. − This is very important considering that some of these chemicals are also surfactants, and foam fractionation is an effective separation method for surfactant removal from a solution containing a low surfactant concentration.…”

Section: Introductionmentioning

confidence: 99%

Model for Investigating Relationships between Surfactant Micropollutant Properties and Their Separation from Liquid in a Bubble Column

et al. 2023

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The removal of surfactant micropollutants, such as dyes, pharmaceuticals, and proteins, through foam is very important in biotechnology and wastewater treatment. The literature shows that previous models consider mass balances within the foam but not the adsorption dynamics of micropollutant surfactants on bubble surfaces in the liquid solution. Thus, the main objective of this work is to examine the removal of surfactant micropollutants in a bubble column considering both mass balance and adsorption dynamics to calculate surfactant transport from the liquid bulk to the bubble surface. This allows investigation of the relationships between surfactant hydrophobicity and surfactant separation efficiency from the liquid. It was found that the removal of the surfactant strongly depends on the dynamic adsorption behavior of surfactant on bubble surfaces, and the highest foam fractionation performance was achieved when the surfactant molecule was highly hydrophobic. This work demonstrates that the adsorption dynamics rather than adsorption thermodynamics on bubble surfaces is critical when modeling the removal of surfactant micropollutants from water solutions.

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Foam fractionation of a recombinant biosurfactant apolipoprotein

Cited by 10 publications

References 33 publications

Recent stationary phase‐based fractionation strategies in proteomic analysis

Recent stationary phase‐based fractionation strategies in proteomic analysis

Gas‐Liquid Flow‐Induced Characteristics of Dispersed Liposome‐Based Particles

Model for Investigating Relationships between Surfactant Micropollutant Properties and Their Separation from Liquid in a Bubble Column

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