Focal adhesion kinase and paxillin promote migration and adhesion to fibronectin by swine skeletal muscle satellite cells

Wang, Dan; Gao, Chun‐qi; Chen, Rong-Qiang; Jin, Can; Li, Hai-chang; Yan, Huichao; Wang, Xiu-Qi

doi:10.18632/oncotarget.9010

Cited by 24 publications

(17 citation statements)

References 36 publications

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“…Actually, phosphorylation of paxillin Tyr31 and Tyr118 regulated actin-dependent polalization and motility of pre-B cell line [ 69 ]. Therefore, increased phosphorylation of paxillin Tyr31 in addition to increased phosphorylation of p130Cas in GD3+ cells [ 67 ] may play roles in the increased invasion ( Fig 3 ) as reported in skeletal muscle satellite cells [ 70 ]. Reduced Tyr181 phosphorylation in addition to low level of p130Cas phosphorylation in GD2+ cells might be involved in the lowered invasion/migration activity, while it is not clear at this moment.…”

Section: Discussionmentioning

confidence: 88%

Differential roles of gangliosides in malignant properties of melanomas

et al. 2018

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Ganglioside GD3 is widely expressed in human malignant melanomas, and has been reported to be involved in the increased cell proliferation and invasion. In this study, we established GM3-, GM2-, GM1-, GD3-, or GD2-expressing melanoma cell lines by transfecting cDNAs of glyscosyltransferases, and effects of individual gangliosides on the cell phenotypes and signals were examined. The phenotypes of established ganglioside-expressing cells were quite different, i.e. cell growth increased as following order; GD2+, GD3+ > GM1+, GM2+, GM3+ cells. Cell invasion activity increased as GD3+ ≧ GM2+ > GM1+, GM3+, GD2+ cells. Intensity of cell adhesion to collagen I (CL-I) and spreading increased as GD2+ >> GD3+, GM1+ > GM2+, GM3+ cells. In particular, cell adhesion of GD2+ cells was markedly strong. As for cell migration velocity, GD2+ cells were slower than all other cells. The immunocytostaining revealed close localization of gangliosides and F-actin in lamellipodia. Immunoblotting of phosphorylated p130Cas and paxillin by serum treatment reveled that these phosphorylations were more increased in GD3+ cells than in GD2+ or GM3+ cells, while phosphorylation of Akt underwent similarly increased phosphorylation between GD3+ and GD2+ cells compared with GM3+ cells. While GD2 and GD3 enhanced cell growth, GD3 might also contribute in cell invasion. On the other hand, GD2 might contribute in the solid fixation of melanoma cells at metastasized sites. These results suggested that individual gangliosides exert distinct roles in the different aspects of melanomas by differentially regulating cytoskeletons and signaling molecules.

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Section: Discussionmentioning

confidence: 88%

Differential roles of gangliosides in malignant properties of melanomas

et al. 2018

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“…The method used to isolate SCs was previously described [ 17 , 39 , 40 ], and immunocytochemistry was used to identify SC purity by paired box protein 7 (Pax7). Immunocytochemistry analysis showed the positive cells for the Pax7 polyclonal antibody (Wuhan Huamei Biotech Co. Ltd, Wuhan, China) accounted for 90% of total cells (Figure 3 ).…”

Section: Methodsmentioning

confidence: 99%

Differentiation capacities of skeletal muscle satellite cells in Lantang and Landrace piglets

Gao

Jin

et al. 2017

Oncotarget

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We isolated and cultured satellite cells (SCs) from the longissimus dorsi muscles of 1-day-old male Landrace and Lantang piglets to compare the SC differentiation capacity in the two breeds. Lantang piglets yielded more (P < 0.05) SCs per gram of muscle than Landrace piglets (5.2 ± 0.9×104 vs. 2.4 ± 0.2×104). Transcription of the differentiation markers myogenin and myosin heavy chain I (MyHC I) in the longissimus dorsi muscle was higher in Lantang than Landrace piglets (P < 0.05). Protein levels of myogenin (P < 0.05), MyHC I (P < 0.05), and myogenic regulatory factor 4 (P = 0.07) were higher in Lantang than Landrace piglet SCs after 72 h of differentiation. Creatine kinase activity was higher in Lantang than Landrace piglet SCs after 24, 48, and 72 h of differentiation (P < 0.05), and there was a greater fusion index in Landrace piglet SCs after 72 h of differentiation. In addition, phosphorylation of Akt, mTOR, S6K1, S6, and 4EBP1 was lower in Lantang than Landrace piglet SCs (P < 0.05). Thus differentiation was more extensive in Lantang than Landrace piglet SCs, but expression of the mTOR signaling pathway was lower in Lantang piglet SCs, suggesting mTOR signaling may inhibit myogenic differentiation. These findings reveal that mTOR signaling is a factor in myogenesis and imply that mTOR could potentially serve as an activator of myoblast differentiation and muscle regeneration.

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“…These then becomes focal complexes, which are more stable and necessary adhesions that connect the lamellipodium to the lamella. This is referred to as early-stage adhesion [28][29][30]. At this point, Rho A activity is downregulated while that of Rac1 is upregulated.…”

Section: Altered Rho-gtpasementioning

confidence: 99%

The Role of Rho GTPases in Motility and Invasion of Glioblastoma Cells

Al-Koussa

Atat

Jaafar

et al. 2020

Analytical Cellular Pathology

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Astrocytomas are primary malignant brain tumors that originate from astrocytes. Grade IV astrocytoma or glioblastoma is a highly invasive tumor that occur within the brain parenchyma. The Rho family of small GTPases, which includes Rac1, Cdc42, and RhoA, is an important family whose members are key regulators of the invasion and migration of glioblastoma cells. In this review, we describe the role played by the Rho family of GTPases in the regulation of the invasion and migration of glioblastoma cells. Specifically, we focus on the role played by RhoA, Rac1, RhoG, and Cdc42 in cell migration through rearrangement of actin cytoskeleton, cell adhesion, and invasion. Finally, we highlight the importance of potentially targeting Rho GTPases in the treatment of glioblastoma.

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Focal adhesion kinase and paxillin promote migration and adhesion to fibronectin by swine skeletal muscle satellite cells

Cited by 24 publications

References 36 publications

Differential roles of gangliosides in malignant properties of melanomas

Differential roles of gangliosides in malignant properties of melanomas

Differentiation capacities of skeletal muscle satellite cells in Lantang and Landrace piglets

The Role of Rho GTPases in Motility and Invasion of Glioblastoma Cells

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