Focal contacts of spreading platelets with the substratum

Alexandrova, Antonina Y.; Vasiliev, J. M.

doi:10.1016/0014-4827(84)90468-3

Cited by 14 publications

(2 citation statements)

References 12 publications

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“…Many proteins found in fibroblast focal adhesions are present in large amounts in blood platelets (Rosenberg et al 1981;Collier & Wang 1982;Koteliansky et al 1984;O'Halloran et al 1985;Rosenfeld et al 1985). Platelets form focal adhesions when plated on appropriate substrates (Zobel & Woods 1983;Alexandrova & Vasiliev 1984), and adhesion to the ECM is essential to their function in vivo. For example, platelet adhesion to the subendothelial ECM, exposed by blood vessel injury, triggers platelet activation.…”

Section: Occurrence Of Focal Adhesions In Vivomentioning

confidence: 99%

Focal Adhesions: Transmembrane Junctions Between the Extracellular Matrix and the Cytoskeleton

Burridge¹,

Fath²,

Kelly³

et al. 1988

Annu. Rev. Cell. Biol.

2,053

1,313

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Section: Occurrence Of Focal Adhesions In Vivomentioning

confidence: 99%

Focal Adhesions: Transmembrane Junctions Between the Extracellular Matrix and the Cytoskeleton

Burridge¹,

Fath²,

Kelly³

et al. 1988

Annu. Rev. Cell. Biol.

2,053

1,313

View full text Add to dashboard Cite

Section: Occurrence Of Focal Adhesions In Vivomentioning

confidence: 99%

Focal Adhesions: Transmembrane Junctions Between The Extracellular Matrix And The Cytoskeleton

Burridge¹

1988

Annual Review of Cell and Developmental Biology

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Vinculin in relation to stress fibers in spread platelets

Nachmias

Golla

1991

Cell Motil. Cytoskeleton

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To investigate the function of vinculin in blood platelets, we studied its localization in relation to other cytoskeletal proteins as well as its state of phosphorylation in platelets allowed to spread on fibrinogen-coated surfaces. By 5 minutes after loading the platelets onto the surfaces the 47 and 20 kDa polypeptides became phosphorylated, indicating activation. By 30 minutes, platelets formed small, typical bundles of fibers which stained brilliantly with rhodamine phalloidin. Myosin and tropomyosin, detected with specific antibodies, were localized in periodic arrays along these bundles. By indirect immunofluorescence, a discrete patch of vinculin was observed at each end of every actin-containing bundle. Vinculin phosphorylation was not detected in immunoprecipitates protected against phosphatases. Interference reflection images showed that regions of close binding to the substratum (adhesion plaques) closely matched the vinculin staining sites. Talin appeared diffusely localized. It could be shown to be present in the plaques when platelets were stabilized with ZnCl2 by the method of Geiger and then sonicated to remove some of the surface membrane. Localizations of vinculin and myosin were unaltered by this treatment. Talin phosphorylation or proteolysis could not account for vinculin translocation. We conclude that platelets, in response to an appropriate physiological surface, form typical actin bundles with vinculin at the termination of each bundle, in close relation to adhesion plaques. The signal for this translocation does not appear to depend on phosphorylation of vinculin or on phosphorylation or proteolysis of talin. Our findings support the conclusion that in platelets, as in nucleated cells, vinculin serves as at least part of the connection between bundled actin fibers and the extracellular matrix. Such a connection seems required for platelets' known ability to exert tension on surfaces.

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Focal contacts of spreading platelets with the substratum

Cited by 14 publications

References 12 publications

Focal Adhesions: Transmembrane Junctions Between the Extracellular Matrix and the Cytoskeleton

Focal Adhesions: Transmembrane Junctions Between the Extracellular Matrix and the Cytoskeleton

Focal Adhesions: Transmembrane Junctions Between The Extracellular Matrix And The Cytoskeleton

Vinculin in relation to stress fibers in spread platelets

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