A normal bone marrow microenvironment plays a very important role in the normal
functioning of hematopoietic stem cells. Once disturbed, this microenvironment can become
favorable for the occurrence of blood disorders, cancers, and other diseases. Therefore,
further studies on the bone marrow microenvironment should be performed to reveal
regulatory and stem cell fate determination mechanisms and promote the development of bone
marrow transplantation, tissue repair and regenerative medicine, and other fields. A small
animal model for further research is also urgently needed. In this study, an electric
shock device was designed to elicit a femur bone marrow microenvironment injury in mice. A
wire was inserted into the distal femur but not into the proximal femur, and the bone
marrow microenvironment was evidently damaged by application of 100 ± 10 V for 1.5 ± 0.5
min ; mortality, however, was low in the mice. Gross observation, hematoxylin and eosin
staining, immunohistochemistry, bright-field microscopy, and micro-CT scanning were also
conducted. A large number of new blood capillaries and sinusoids appeared in the injured
distal femur after 2 weeks. The capillaries in the injured femur disappeared after 4
weeks, and mature blood vessels were scattered throughout the injured area. Red blood
cells disappeared, and the cellular structure and trabecular bone were better than those
observed 2 weeks previously. Thus, we developed a simply operated, accurate, reliable, and
easily controlled small animal model as a good technical platform to examine angiogenesis
and segmentation damage in the bone marrow microenvironment.