Focus on “Regulation of transferrin-induced endocytosis  by wild-type and C282Y-mutant HFE in transfected HeLa cells”

Okamoto, Curtis T.

doi:10.1152/ajpcell.00009.2002

Cited by 14 publications

(4 citation statements)

References 10 publications

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“…We found that FTIR-SPR in the mid-IR can detect, in realtime, endocytic processes related to Tfn-induced formation of early endocytic vesicles. Our SPR results correlate with independent time-resolved capacitance data (5)(6)(7), suggesting that the SP wave indeed senses Tfn-induced generation of endocytic carriers. Novel information was obtained about the cholesterol dependent dynamics of endocytic vesicle formation and consumption, e.g., by recycling back to the plasma membrane.…”

Section: Introductionsupporting

confidence: 77%

“…The capacitance technique measures changes in the plasma membrane surface area. The endocytic vesicles are derived from the cell surface and therefore decrease the area of that surface (5). Therefore, the capacitance experiments sense indirectly the production of endocytic vesicles stimulated by Tfn binding to its receptor.…”

Section: Similarity To Capacitance Measurements the Kinetics Ofmentioning

confidence: 99%

“…Although the empty receptor is indeed efficiently internalized via clathrin-coated pits, accumulating evidence suggests that Tfn binding may regulate certain aspects of the TfnR endocytic pathway. First, Tfn addition stimulates TfnR internalization (4); second, time-resolved capacitance measurements suggest that cell exposure to Tfn stimulates the production of endocytic vesicles (5)(6)(7); third, Tfn stabilizes the assembly, growth, and budding of clathrin-coated pits (8). Hence, the mechanism by which Tfn affects its own endocytosis definitely merits further investigation.…”

Section: Introductionmentioning

confidence: 99%

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Real-Time Monitoring of Transferrin-Induced Endocytic Vesicle Formation by Mid-Infrared Surface Plasmon Resonance

Yashunsky

Shimron

Lirtsman

et al. 2009

Biophysical Journal

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We report on the application of surface plasmon resonance (SPR), based on Fourier transform infrared spectroscopy in the mid-infrared wavelength range, for real-time and label-free sensing of transferrin-induced endocytic processes in human melanoma cells. The evanescent field of the mid-infrared surface plasmon penetrates deep into the cell, allowing highly sensitive SPR measurements of dynamic processes occurring at significant cellular depths. We monitored in real-time, infrared reflectivity spectra in the SPR regime from living cells exposed to human transferrin (Tfn). We show that although fluorescence microscopy measures primarily Tfn accumulation in recycling endosomes located deep in the cell's cytoplasm, the SPR technique measures mainly Tfn-mediated formation of early endocytic organelles located in close proximity to the plasma membrane. Our SPR and fluorescence data are very well described by a kinetic model of Tfn endocytosis, suggested previously in similar cell systems. Hence, our SPR data provide further support to the rather controversial ability of Tfn to stimulate its own endocytosis. Our analysis also yields what we believe is novel information on the role of membrane cholesterol in modulating the kinetics of endocytic vesicle biogenesis and consumption.

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Section: Introductionsupporting

confidence: 77%

Section: Similarity To Capacitance Measurements the Kinetics Ofmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Real-Time Monitoring of Transferrin-Induced Endocytic Vesicle Formation by Mid-Infrared Surface Plasmon Resonance

Yashunsky

Shimron

Lirtsman

et al. 2009

Biophysical Journal

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“…The C282Y HFE mutation affects the b 2 microglobulin chain of HFE, allowing a higher rate of transferrin binding to the TfR1 transferrin receptor and thus a higher rate of internalization of the Tf-TfR1 complex (8). The frequency of this mutation is decreased in southeastern Europe (1% to 5%) compared with northwestern Europe (6% to 10%) (9).…”

mentioning

confidence: 99%

The impact of hemochromatosis mutations and transferrin genotype on gonadotropin serum levels in infertile men

Buretić‐Tomljanović

Vlastelić

Badovinac

et al. 2009

Fertility and Sterility

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Homocysteine is transported by the microvillous plasma membrane of human placenta

Tsitsiou

Sibley

D'Souza

et al. 2010

J of Inher Metab Disea

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Elevated maternal plasma concentrations of homocysteine (Hcy) are associated with pregnancy complications and adverse neonatal outcomes. The postulate that we wish to advance here is that placental transport of Hcy, by competing with endogenous amino acids for transporter activity, may account for some of the damaging impacts of Hcy on placental metabolism and function as well as fetal development. In this article, we provide an overview of some recent studies characterising the transport mechanisms for Hcy across the microvillous plasma membrane (MVM) of the syncytiotrophoblast, the transporting epithelium of human placenta. Three Hcy transport systems have been identified, systems L, A and y + L. This was accomplished using a strategy of competitive inhibition to investigate the effects of Hcy on the uptake of wellcharacterised radiolabelled substrates for each transport system into isolated MVM vesicles. The

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Focus on “Regulation of transferrin-induced endocytosis by wild-type and C282Y-mutant HFE in transfected HeLa cells”

Cited by 14 publications

References 10 publications

Real-Time Monitoring of Transferrin-Induced Endocytic Vesicle Formation by Mid-Infrared Surface Plasmon Resonance

Real-Time Monitoring of Transferrin-Induced Endocytic Vesicle Formation by Mid-Infrared Surface Plasmon Resonance

The impact of hemochromatosis mutations and transferrin genotype on gonadotropin serum levels in infertile men

Homocysteine is transported by the microvillous plasma membrane of human placenta

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