Focused Ultrasound Treatment of a Spheroid In Vitro Tumour Model

Landgraf, Lisa; Kozlowski, Adam; Zhang, Xinrui; Fournelle, Marc; Becker, Franz-Josef; Tretbar, Steffen; Melzer, Andreas

doi:10.3390/cells11091518

Cited by 13 publications

(12 citation statements)

References 24 publications

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“…A full description regarding Tukey’s multiple comparisons test can be found in the supporting information. Validating our findings, researchers discovered that US inhibited spheroid growth, metabolic activity, disrupted spheroid integrity, and increased DNA double strand breaks, leading to damage in human prostate cancer (PC-3) and GBM (U87) cell lines [33].…”

Section: Resultsmentioning

confidence: 86%

Ultrasound-mediated drug diffusion, uptake, and cytotoxicity in glioblastoma 3D tumour sphere model

Wanigasekara,

Mondala,

Cullen

et al. 2023

Preprint

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A myriad of biological effects can be stimulated by the ultrasound (US) for the treatment of cancer. The objective of our research was to investigate the effect of ultrasound alone and in combination with the chemotherapeutic drugs such as doxorubicin (DOX) and temozolomide (TMZ) on human glioblastoma (GBM) and the human epidermoid carcinoma cancer 2D and 3D cell cultures. Results indicated that the US 96-probe device could induce tumour sphere cytotoxicity in a dosage- and time-dependent manner, with multiple treatments augmenting this cytotoxic effect. With enhanced cytotoxicity, US decreased tumour sphere growth metabolic activity, disrupted spheroid integrity, and heightened the occurrence of DNA double strand breaks, resulting in damage to tumour spheres and an inability to rebuild tumour spheres after multiple US treatments. The combination of US and TMZ/DOX enhanced the efficiency of treatment for GBM and epidermoid carcinoma by enhancing induced cytotoxicity in 3D tumour spheres compared to 2D monolayer cells, and also by increasing the incubation time, which is the most crucial way to differentiate between the effectiveness of drug treatment with and without US. In conclusion, our data demonstrate that US enhances drug diffusion, uptake, and cytotoxicity using 3D spheroid models when compared with 2D cultures. It also demonstrates the significance of 3D cell culture models in drug delivery and discovery research.

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Section: Resultsmentioning

confidence: 86%

Ultrasound-mediated drug diffusion, uptake, and cytotoxicity in glioblastoma 3D tumour sphere model

Wanigasekara,

Mondala,

Cullen

et al. 2023

Preprint

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“…The Lorentz grasping microcoils were also effective at ablating tumor tissue at 16% cell viability with low power (0.69 mW). This value is widely acceptable for 3D cell cultures [26], [27]. Moreover, spheroid cell viability could be decreased by applying current or measuring viability over a longer period of time.…”

Section: Discussionmentioning

confidence: 97%

Design and Development of a Lorentz Force-Based MRI-Driven Neuroendoscope

Phelan¹,

Dogan²,

Lazović³

et al. 2022

Preprint

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The introduction of neuroendoscopy, microneurosurgery, neuronavigation, and intraoperative imaging for surgical operations has made significant improvements over other traditionally invasive surgical techniques. The integration of magnetic resonance imaging (MRI)-driven surgical devices with intraoperative imaging and endoscopy can enable further advancements in surgical treatments and outcomes. This work proposes the design and development of an MRI-driven endoscope leveraging the high (3-7 T), external magnetic field of an MR scanner for heat-mitigated steering within the ventricular system of the brain. It also demonstrates the effectiveness of a Lorentz force-based grasper for diseased tissue manipulation and ablation. Feasibility studies show the neuroendoscope can be steered precisely within the lateral ventricle to locate a tumor using both MRI and endoscopic guidance. Results also indicate grasping forces as high as 31 mN are possible and power inputs as low as 0.69 mW can cause cancerous tissue ablation. These findings enable further developments of steerable devices using MR imaging integrated with endoscopic guidance for improved outcomes.

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“…As a matter of fact, focused ultrasound can be employed either to destroy cancerous cells by heating or as an adjuvant therapy, in combination with chemotherapy or radiation therapy. The main points of values of FUS are the non-invasiveness, incision-free, controllability via real-time MR guidance and the capacity to activate the immune system [54]. The rst non-invasive thermal ablation of a brain tumor in human was realized by Coluccia…”

Section: Discussionmentioning

confidence: 99%

Sonodynamic therapy and Magnetic Resonance-guided Focused Ultrasound: new therapeutic strategy in Glioblastoma

Bonosi

Marino

Benigno

et al. 2023

Preprint

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Glioblastoma (GB) is one of the most aggressive and difficult-to-treat brain tumors, with a poor prognosis and limited treatment options. In recent years, sonodynamic therapy (SDT) and magnetic resonance focused ultrasound (MRgFUS) have emerged as promising approaches for the treatment of GB. SDT uses ultrasound waves in combination with a sonosensitizer to selectively damage cancer cells, while MRgFUS delivers high-intensity ultrasound waves to precisely target tumor tissue and disrupt the blood-brain barrier to enhance drug delivery. In this review, we explore the potential of SDT as a novel therapeutic strategy for GBM. We discuss the principles of SDT, its mechanisms of action, and the preclinical and clinical studies that have investigated its use in Gliomas. We also highlight the challenges, the limitations, and the future perspectives of SDT. Overall, SDT and MRgFUS hold promise as novel and potentially complementary treatment modalities for GB. Further research is needed to optimize their parameters and determine their safety and efficacy in humans, but their potential for selective and targeted tumor destruction makes them an exciting area of investigation in the field of brain cancer therapy.

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Focused Ultrasound Treatment of a Spheroid In Vitro Tumour Model

Cited by 13 publications

References 24 publications

Ultrasound-mediated drug diffusion, uptake, and cytotoxicity in glioblastoma 3D tumour sphere model

Ultrasound-mediated drug diffusion, uptake, and cytotoxicity in glioblastoma 3D tumour sphere model

Design and Development of a Lorentz Force-Based MRI-Driven Neuroendoscope

Sonodynamic therapy and Magnetic Resonance-guided Focused Ultrasound: new therapeutic strategy in Glioblastoma

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