Folate and vitamin B12 status is associated with insulin resistance and metabolic syndrome in morbid obesity

Li, Zhen; Guéant-Rodríguez, Rosa-María; Quilliot, Didier; Sirveaux, Marie-Aude; Meyre, David; Guéant, Jean‐Louis; Brunaud, Laurent

doi:10.1016/j.clnu.2017.07.008

Cited by 86 publications

(61 citation statements)

References 38 publications

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“…Guéant & Alpers (5) and Garcia et al (53) using an animal model demonstrated that the deficiency of methyl radical and other cofactors, such as B 12 , impairs the oxidation of the fatty acids, increases the stress on the endoplasmic reticulum and decreases the expression of sirtuin 1, a protein which plays a key role in molecular mechanisms of obesity. Li et al (20) proposed that the impaired conversion of methylmalonic acid (MMA) to succinyl-CoA, a B 12 -dependent process, may be associated with MMA accumulation, which could lead to an increase in lipogenesis, in addition to insulin resistance. In a study that included both in vivo and in vitro observations, Adaikalakoteswari et al (18) suggested that the B 12 plays a role in epigenetic regulation by altering circulating microRNA during adipocyte differentiation that results in adipogenesis and adverse metabolic phenotype.…”

Section: Discussionmentioning

confidence: 99%

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Vitamin B12 status in kidney transplant recipients: association with dietary intake, body adiposity and immunosuppression

et al. 2019

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The aim of the present study was to evaluate the prevalence of vitamin B12 (B12) deficiency in kidney transplant recipients (KTR) and its possible association with B12 dietary intake, body adiposity and immunosuppressive drugs. In this cross-sectional study, we included 225 KTR, aged 47·50 (sd 12·11) years, and 125 (56 %) were men. Serum levels of B12 were determined by chemiluminescent microparticle intrinsic factor assay and the cut-off of 200 pg/ml was used to stratify KTR into B12-sufficient or B12-deficient group. B12 dietary intake was evaluated by three 24 h dietary recalls and was considered adequate when ≥2·4 μg/d. Body adiposity was estimated after taking anthropometric measures and using the dual-energy X-ray absorptiometry (DXA) method. B12 deficiency was seen in 14 % of the individuals. B12-deficient group, compared with the B12-sufficient group, exhibited lower intake of B12 (median 2·42 (interquartile range (IQR) 1·41–3·23) v. 3·16 (IQR 1·94–4·55) μg/d, P = 0·04) and higher values of waist circumference (median 96·0 (IQR 88·0–102·5) v. 90·0 (IQR 82·0–100·0) cm, P = 0·04). When the analysis included only women, B12 deficiency was associated with higher total and central body adiposity measurements obtained with anthropometry (BMI, body adiposity index, waist and neck circumferences) and DXA (total and trunk body fat). Among individuals with adequate intake of B12, the deficiency of this vitamin was more frequently seen in those using mycophenolate mofetil (MMF) (17 %) v. azathioprine (2 %), P = 0·01. In conclusion, the prevalence of B12 deficiency in KTR was estimated as 14 % and was associated with reduced intake of B12 as well as higher adiposity, especially in women, and with the use of MMF.

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Section: Discussionmentioning

confidence: 99%

“…authors (14)(15)(16)(17)(18)(19)(20)(21) . However, to date, no study has evaluated the association of serum B 12 levels with excessive body weight using a 'gold standard' method to evaluate body adiposity such as dual-energy X-ray absorptiometry (DXA).…”

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confidence: 99%

Vitamin B12 status in kidney transplant recipients: association with dietary intake, body adiposity and immunosuppression

et al. 2019

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“…In a recent study published by Li et al [12] , the way vitamin B12 deficiency affects insulin resistance was described as follows: B12 deficiency leads to insufficiency in the syn-thesis of methionine by inducing leakage of cellular folate, which increases the stress in the endoplasmic reticulum by causing deficiency of oxidation of free fatty acids.…”

Section: Discussionmentioning

confidence: 99%

“…Because B12 is a cofactor in the conversion of methylmalonic acid to succinylcholine, methylmalonic acid accumulates in B12 deficiency, and causes lipogenesis and insulin resistance [12] . Hence, in obese children, lower vitamin B12 levels are seen to no less a degree.…”

Section: Discussionmentioning

confidence: 99%

Is there any relationship between insulin resistance and vitamin B12 deficiency in obese children?

Dursun¹

2018

Haydarpasa Numune Med J

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Introduction: The increase in the incidence of obesity leads to an increase in the frequency of obesity-related complications such as insulin resistance (IR), hypertension, and hyperlipidemia. This study aimed to examine the relationship between B12 levels and IR in obese patients. Methods: A total of 110 children aged 10-16 years who presented to pediatric endocrinology outpatient clinic for obesity were enrolled. The homeostasis model of assessment-insulin resistance (HOMA-IR) made the diagnosis of IR. The patients who had previously received B12 and metformin treatment were excluded from the study. The vitamin B12 levels between patients with and without IR were compared. The correlation between B12 levels and IR was evaluated. Results: When patients with IR were compared with those without IR, a statistically significant difference was observed in serum B12 levels (p=0.019). A strong negative correlation was observed between vitamin B12 levels and IR (r=−0.259, p=0.008). Discussion and Conclusion: We found that vitamin B12 levels were significantly lower in obese children with IR. Metformin used for IR is known to have lowered the absorption of vitamin B12. In addition to screening of IR and other co-morbidities in obesity, the lack of vitamin B12 should also be investigated. If deficient, vitamin B12 should be replaced before starting metformin treatment. Further studies are needed to examine the effect of vitamin B12 deficiency on the pathogenesis of IR.

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“…2 Population data reveal a great variation in the distribution of hyperhomocysteinemia, ranging from 0% to 86% 2,3 attributed to a polymorphism in methylenetetrahydrofolate reductase gene (MTHFR; rs1801133) and variable folate and/ or vitamin B12 levels. 4,5 Being at the axis of DNA synthesis and methyla-tion cycle, homocysteine metabolism (in close proximity with MTHFR) plays an important role in lipid metabolism, immune activation and other biological reactions. [6][7][8] Hypomethylation of homocysteine has been associated with dyslipidemia, atherosclerosis, hypertension, and all cause mortality in different populations of the world.…”

Section: Introductionmentioning

confidence: 99%

Impaired Homocysteine Metabolism Associated with High Plasma Interleukin- 17A Levels, a Pro-Atherogenic Marker, in an Endogamous Population of North India

et al. 2018

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Background: Impaired homocysteine metabolism (IHM; hyperhomocysteinemia) has been linked with many complex disorders like cardiovascular diseases and immunological disturbances. However, studies understanding IHM in light of pro- and anti-atherogeneic markers like Interleukin-17A & -10 (IL-17A & IL-10) and Forkhead box p3 (Foxp3, a master transcription factor) are scarce.Aim: In our present study, we aimed to understand the relation of IHM with plasma IL-17A and IL-10 levels and Foxp3 mRNA expression in peripheral blood mononuclear cells (PBMCs) from an endogamous population (Jats of Haryana, North India) with high prevalence of IHM without the concurrence of significant adverse cardiovascular outcomes.Methods: Forty (40) clinically healthy individuals, unrelated up to first cousins, were recruited and were subjected to demographic, physiological and anthropometric profiling, followed by intravenous blood sample collection (fasting) and lipid profiling. Plasma homocysteine levels were estimated and individuals with homocysteine levels ≥ 15umol/L and <15umol/L were categorized as the impaired homocysteine metabolism group (IHM, n=30) and normal homocysteine metabolism group (NHM, n=10) respectively. Plasma folate and vitamin B12 and MTHFR C677T (methylenetetrahydrofolate reductase) polymorphism were detected. Relative mRNA expression of Foxp3 in PBMCs (normalized to 18S) was quantitated using SyBR green technology. Plasma IL-10 & 17 levels were estimated by ELISA assays.Results and Conclusion: None of the physiological, anthropometric and lipid variables were different between the two groups. Foxp3 mRNA expression levels were relatively lower, and plasma IL-10 levels were found to be comparable among IHM and NHM group. However, significantly higher IL-17A levels and relatively high LDL cholesterol levels were present in the IHM group as compared with NHM. Our findings suggest that the Jats of Haryana, North India, exhibiting high levels of homocysteine, might also carry the high IL-17A -pro-atherogenic marker, suggesting an increasing burden of pre-morbid condition. This apparently does not reach to significant mortality/morbidity attributed to the counter action or balancing act of IL-10 (an anti-atherogenic marker). This further suggests environment-influenced epigenetic control mechanisms of the targeted genes in the present population.Ethn Dis. 2018;28(4):525-530; doi:10.18865/ ed.28.4.525

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Folate and vitamin B12 status is associated with insulin resistance and metabolic syndrome in morbid obesity

Cited by 86 publications

References 38 publications

Vitamin B12 status in kidney transplant recipients: association with dietary intake, body adiposity and immunosuppression

Vitamin B12 status in kidney transplant recipients: association with dietary intake, body adiposity and immunosuppression

Is there any relationship between insulin resistance and vitamin B12 deficiency in obese children?

Impaired Homocysteine Metabolism Associated with High Plasma Interleukin- 17A Levels, a Pro-Atherogenic Marker, in an Endogamous Population of North India

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