Folate-Binding Proteins

Colman, Neville; Herbert,

doi:10.1146/annurev.me.31.020180.002245

Cited by 29 publications

(20 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HFBP has been known to be distributed in various biological fluids and tissues including milk, plasma or serum, cerebrospinal fluid, saliva, urine, liver, kidney, small intestine, spleen, placenta, choroid plexus, neutrophils, granulocytes, and various cancer cells in several mammalian species [2,3,5,9,19]. Although some of these HFBPs were purified and their biochemical properties were characterized, their physiological functions in folate distribution, transport, and intracellular uptake are still unclear [2,3,5,9,19].…”

mentioning

confidence: 99%

“…Although some of these HFBPs were purified and their biochemical properties were characterized, their physiological functions in folate distribution, transport, and intracellular uptake are still unclear [2,3,5,9,19]. Due to its uniquely high capacity of specific folate binding, further research is required concerning porcine plasma HFBP.…”

mentioning

confidence: 99%

“…Free concentration of 3 HFA was determined by liquid scintillation counting (Aloka Liquid Scintillation System, model SC-700, Tokyo, Japan) using scintillation solvent (Biofluor, Biotechnology systems, NEN ® Research Products, MA). Total folate levels were also determined by using HPLC-ECD system for THF and 5MF, and liquid scintillation counting for 3 HFA as described previously [12,13].…”

mentioning

confidence: 99%

“…Binding kinetic analysis: In this analysis, to obtain dissociation constants of HFBP to folates, the maximum binding capacity of the prepared HFBP solution was set to be about 160 nM for THF and 5MF, and about 18 nM for 3 HFA binding. The concentrations of free (Cf) and bound (Cb) folate were depicted in a Scatchard plot.…”

mentioning

confidence: 99%

“…The maximum binding capacity of the prepared HFBP solution was adjusted to be approximately 18 nM. Binding activities of 3 …”

mentioning

confidence: 99%

See 4 more Smart Citations

Binding Characteristics of Folate to High Affinity Folate Binding Protein Purified from Porcine Serum.

Natsuhori

Okada

Ida

et al. 1999

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. High affinity folate binding protein (HFBP) in porcine serum was purified 2,000-fold to a specific activity of 1.4 nmol of tetrahydrofolic acid (THF) bound per mg of protein, using folic acid (FA) coupled EAH-Sepharose gel affinity chromatography. Binding activity of purified HFBP to folate was examined by ultrafiltration method linked to high-performance liquid chromatography with electrochemical detection or to liquid scintillation counting. Binding affinity of HFBP to folate was characterized by dissociation constants (Kd): 13, 17, and 31 pM for tritiated FA ( 3 HFA), THF, and N 5 -methyltetrahydrofolic acid (5MF), respectively. FA, THF, and 5MF significantly inhibited binding of HFBP to 3 HFA, and according to the magnitude of intensity of the binding inhibition, the order of affinity of each folate was confirmed to be FA > THF > 5MF. Binding activity was rather high and stable for THF and 5MF at pH ranging from 6.0 to 10.0. The binding activity, however, rapidly decreased at pH below 6.0 and over 10.0. No binding activity was observed pH below 3.0 and over 12. Gel filtration analysis showed that the prepared HFBP solution had specific binding activity at around 77 kDa of apparent molecular weight, which was 82 kDa by SDS-PAGE. It is considered that this specific and stable binding enables THF to distribute in porcine plasma.-KEY WORDS: binding protein, folate, serum, swine.

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

“…The maximum binding capacity of the prepared HFBP solution was adjusted to be approximately 18 nM. Binding activities of 3 …”

mentioning

confidence: 99%

See 3 more Smart Citations

Binding Characteristics of Folate to High Affinity Folate Binding Protein Purified from Porcine Serum.

Natsuhori

Okada

Ida

et al. 1999

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

show abstract

Altered Folate Metabolism in Early HIV Infection-Reply

Beach¹,

Mantero-Atienza²,

Eisdorfer³

et al. 1988

JAMA

View full text Add to dashboard Cite

show abstract

Kearns‐sayre syndrome with reduced plasma and cerebrospinal fluid folate

Allen

DiMauro

Coulter

et al. 1983

Annals of Neurology

View full text Add to dashboard Cite

A young woman with Kearns-Sayre syndrome and progressive central nervous system deterioration over 15 years had decreased plasma and cerebrospinal fluid folate levels while receiving phenytoin for a seizure disorder. A muscle biopsy showed a "ragged red fiber" myopathy with reduced muscle carnitine and mitochondrial enzymes. Computed tomographic brain scans showed cerebral white matter hypodensities and bilateral calcification of the basal ganglia. The mechanism for the folate deficiency and altered ratio of plasma to cerebrospinal fluid folate is unknown, but the deficiency may be responsive to replacement therapy.

show abstract

Folate-Binding Proteins

Cited by 29 publications

References 0 publications

Binding Characteristics of Folate to High Affinity Folate Binding Protein Purified from Porcine Serum.

Binding Characteristics of Folate to High Affinity Folate Binding Protein Purified from Porcine Serum.

Altered Folate Metabolism in Early HIV Infection-Reply

Kearns‐sayre syndrome with reduced plasma and cerebrospinal fluid folate

Contact Info

Product

Resources

About