Folate content in foods commonly consumed in Egypt

Hefni, Mohammed E.; Öhrvik, Veronica; Tabekha, M. M.; Witthöft, Cornelia M.

doi:10.1016/j.foodchem.2009.12.044

Cited by 72 publications

(74 citation statements)

References 27 publications

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“…This concentration was in a concentration range similar to that which has been reported by other researchers (27)(28)(29).…”

Section: Methods Validationsupporting

confidence: 89%

“…It is observed that only two of the species including 5-CH3-H4PteGlu1 and 5-HCO-H4PteGlu1 were submitted to quantifi cation and 5-CH3-H4PteGlu1 was the predominant form, representing 80-99% in the seeds and 85-91% in the pods. 5-CH3-H4PteGlu1 has been identifi ed as the predominant folate species in many vegetables, legumes, fruit, bread, milk and meat (19,27,30). The total amount of folates in the immature seeds measured in this study was similar to that in a report from the USDA database (16).…”

Section: Comparison Of the Distribution Of Folate Species And Total Fsupporting

confidence: 83%

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Quantification of Total Folate, Folate Species and Polyglutamyl Folate Distribution in Winged Beans (<i>Psophocarus tetragonolobus</i> (L) DC) from Different Cultivars and Growth Stages by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry

Luo

Duan

Zou

et al. 2017

J Nutr Sci Vitaminol

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Summary Winged beans are an important natural source of some micronutrients. This paper presents the first complete characterization of folate derivatives including polyglutamyl 5-methyltetrahydrofolate (5-CH3-H4PteGlun), folate species and total folate accumulating in pods and immature seeds of winged beans from 9 cultivars and 7 growth stages. 5-CH3-H4PteGlun and folate species were determined with a UHPLC-MS/MS method. Accurate determination of 5-CH3-H4PteGlun and folate species was optimized and validated according to EMA guidelines including method selectivity, sensitivity, linearity, accuracy, precision, matrix effect and carry-over. The level of total folate is in the range of 73-200 g/100 g in the pods and 33-61 g/100 g in the immature seeds. The predominant folate species in winged beans is 5-CH3-H4PteGlu1. 5-CH3-H4PteGlu5 is the major polyglutamyl folate derivative. The level of total folate is increased about 4 fold with advancing maturity. For pods, the chain length is increased with growth which shifts from 5-CH3-H4PteGlu1 in the early stage to 5-CH3-H4PteGlu5 and 5-CH3-H4PteGlu6 in the 7th stage. Our findings demonstrate that winged beans are good source of folate. The validated UHPLC-MS/MS method allows for the determination of 5-CH3-H4PteGlun and folate species from other vegetable matrices.

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“…This concentration was in a concentration range similar to that which has been reported by other researchers (27)(28)(29).…”

Section: Methods Validationsupporting

confidence: 89%

Section: Comparison Of the Distribution Of Folate Species And Total Fsupporting

confidence: 83%

Quantification of Total Folate, Folate Species and Polyglutamyl Folate Distribution in Winged Beans (<i>Psophocarus tetragonolobus</i> (L) DC) from Different Cultivars and Growth Stages by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry

Luo

Duan

Zou

et al. 2017

J Nutr Sci Vitaminol

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show abstract

“…Dietary folate intake was estimated using the USDA database (http://ndb.nal.usda.gov) as no national food composition data for folate is available. For foods, for which no data were available in the US database, folate data determined by HPLC [17] were used. Energy was calculated using national food composition data [18].…”

Section: Assessment Of Dietary Folate Intake Before and During The Studymentioning

confidence: 99%

“…Folate content in intervention foods was analyzed in duplicate every 4 weeks through-out the trial using HPLC-UV/FLD detection [17]. Food samples were extracted using tri-enzyme treatment for beans and di-enzyme treatment for bread, juice, and cookies.…”

Section: Quantification Of Folate Content In Intervention Foodsmentioning

confidence: 99%

Effect of a 12-Week Dietary Intervention with Folic Acid or Folate-Enhanced Foods on Folate Status in Healthy Egyptian Women

Hefni¹,

Shalaby²,

Mohamed³

et al. 2016

FNS

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The Egyptian government introduced wheat-flour fortification with iron and folic acid to reduce the incidence of neural tube defects, but suspended it for technical reasons. We previously developed novel legume foods with enhanced folate content. In this study, we investigated the efficacy of 12-week intervention with folate-enhanced foods versus folic acid supplement in improving folate status in Egyptian women. A randomized, parallel intervention trial with two active groups (n = 19, n = 18) and one blinded control group (n = 20) was executed over 12 weeks. Volunteers received either germinated legume foods and orange juice (≈250 μg/d folate) or folic acid supplement (500 μg/d) or apple juice (0 µg/d folate). Folate status was assessed by erythrocyte and plasma folate and total homocysteine (tHcy) at day 0, and after 8 and 12 weeks of intervention. After 12 weeks, mean plasma folate increased by 14 (P < 0.0001) and 12 (P < 0.0001) nmoL in the folic acid and food group, respectively. Erythrocyte folate concentration increased in the folic acid group from 614 to 912 (P < 0.0001) and in the food group from 631 to 914 nmoL (P < 0.0001). After 12 weeks, 90% of subjects in the folic acid group and 70% in the food group had erythrocyte folate concentrations exceeding 906 nmol/L. tHcy concentration was decreased by 20% (P = 0.007) and 18% (P = 0.006) in the folic acid and food group, respectively, but remained unchanged in the control group during intervention. Folate-enhanced foods effectively improve folate status in women of reproductive age. These foods could be used as a complement to folic acid fortification.

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“…Folate quantification in untreated, germinated and oven-dried germinated cereal grains was performed in duplicate after di-enzyme extraction (Hefni et al, 2010). Approximately 0.5e1 g milled sample was extracted (in duplicate) in a boiling water bath for 12 min in 15 mL phosphate buffer after addition of thermostable aamylase (60 mL).…”

Section: Sample Pretreatmentmentioning

confidence: 99%

Effect of germination and subsequent oven-drying on folate content in different wheat and rye cultivars

Hefni

Witthöft

2012

Journal of Cereal Science

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Folate content in foods commonly consumed in Egypt

Cited by 72 publications

References 27 publications

Quantification of Total Folate, Folate Species and Polyglutamyl Folate Distribution in Winged Beans (<i>Psophocarus tetragonolobus</i> (L) DC) from Different Cultivars and Growth Stages by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry

Quantification of Total Folate, Folate Species and Polyglutamyl Folate Distribution in Winged Beans (<i>Psophocarus tetragonolobus</i> (L) DC) from Different Cultivars and Growth Stages by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry

Effect of a 12-Week Dietary Intervention with Folic Acid or Folate-Enhanced Foods on Folate Status in Healthy Egyptian Women

Effect of germination and subsequent oven-drying on folate content in different wheat and rye cultivars

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