Folate-deficiency induced acyl-CoA synthetase short-chain family member 2 increases lysine crotonylome involved in neural tube defects

Wang, Shan; Zeng, Yubing; He, Xuejia; Liu, Fan; Pei, Pei; Zhang, Ting

doi:10.3389/fnmol.2022.1064509

Cited by 6 publications

(2 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By comparing normal mice with NTD mice, we found that 223 proteins were upregulated and 336 proteins were downregulated. Folate and NTD-related shifts occur in commonly known NTD-related pathways such as the Hedgehog signaling pathway ( Murdoch et al, 2010 ), the Wnt signaling pathway, the P53 signaling pathway ( Patterson et al, 2006 ), the PI3K-Akt signaling pathway ( Tian et al, 2022 ), the cAMP signaling pathway ( Shimada and Mukhopadhyay, 2017 ), the AMPK signaling pathway ( Zhang et al, 2021 ), the sphingolipid signaling pathway ( Callihan et al, 2012 ; Lai et al, 2016 ), the Hippo signaling pathway ( Manderfield et al, 2014 ; Wang et al, 2016 ), the one-carbon pool mediated by folate ( Copp et al, 2013 ), axon guidance ( Connor et al, 2005 ), glycolysis and gluconeogenesis ( AdamsMJTNEjom, 1984 ; Wang et al, 2023 ), purine metabolism ( Imbard et al, 2013 ), and some underappreciated pathways, such as taste transduction and ribosome.…”

Section: Discussionmentioning

confidence: 99%

Multiplexing of TMT labeling reveals folate-deficient diet-specific proteome changes in NTDs

Pei,

Shen,

et al. 2024

Front. Cell Dev. Biol.

Self Cite

View full text Add to dashboard Cite

Introduction: In the early stage of embryonic development, the neural tube (NT) cannot be closed properly due to some complex factors, including environmental factors, genetic factors, and the relationship between various factors, leading to the occurrence of neural tube defects (NTDs).Methods: In this study, we induced a mouse model of NTDs by feeding mice with a low-folate diet and intraperitoneally injecting them with 1.5 mg/kg methotrexate on E7.5. Fetal mice were achieved at E13.5, and we extracted proteins from brain tissues with trypsin digestion. After enzymatic digestion, peptides were labeled with TMT/iTRAQ and separated in high-performance liquid chromatography (HPLC) for subsequent liquid chromatography tandem mass spectroscopy (LC-MS/MS) analysis. We used gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotation to analyze proteomic changes and analyze the functional enrichment of differentially expressed proteins (DEPs) in the NTD mice tissues.Results: A low-folate-induced mouse model was successfully constructed. Folate was used as a sensitizing agent, and the teratogenicity rate of the NTD fetal mice increased to 36.5% when the concentration of methotrexate was at 1.5 mg/kg. Mass spectrometry was used to identify 6,614 proteins, and among them, 5,656 proteins were quantified. In the following proteomic analysis, GO classification and KEGG pathway enrichment analysis were conducted, and heatmaps were drawn for differentially expressed proteins (DEPs). The main pathways associated with NTDs, such as the Hedgehog, Wnt, p53, and Hippo signaling pathways and the one-carbon pool mediated by folate, can be identified through a protein–protein interaction (PPI) network. It was also found that the regulation of ribosomal proteins, such as RPL13 and RPL14, which are upregulated in NTDs, has a certain impact on neural tube development.Discussion: Our results revealed proteomic changes in the tissues of low-folate-induced NTD mice. Validation showed that ribosomal proteins play a regulatory role during the development of NTDs and provides new ideas for the pathogenesis and preventive measures of NTDs.

show abstract

Section: Discussionmentioning

confidence: 99%

Multiplexing of TMT labeling reveals folate-deficient diet-specific proteome changes in NTDs

Pei,

Shen,

et al. 2024

Front. Cell Dev. Biol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Volcano plots were used to show distribution of DEPs between different samples ( 28 ). Peptide length distribution analysis was performed to evaluate the conformity of peptide lengths identified by mass spectrometry based on enzymatic hydrolysis and mass spectrometry fragmentation mode ( 29 ). Moreover, heatmap was generated to visualize the protein expression patterns in the proteomics study.…”

Section: Methodsmentioning

confidence: 99%

ORMDL3‑mediated bronchial epithelial pyroptosis leads to lung inflammation in obese mice with asthma

Liu,

Sun,

Zhou

et al. 2023

Mol Med Rep

Self Cite

View full text Add to dashboard Cite

asthma associated with obesity is a chronic disease that poses a threat to health in children and results in severe wheezing, earlier airway remodeling and increased insensitivity to hormone therapy compared with those who only have asthma. despite its clinical importance, knowledge on the underlying mechanisms of this disease is limited. The present study aimed to elucidate the pathogenesis of asthma associated with obesity using a murine model. a total of 30 female BalB/c mice were divided into three groups: normal, mice with asthma and obese mice with asthma. obese mice with asthma were fed a high-fat diet to induce obesity. Mice with asthma were sensitized and challenged with ovalbumin (oVa). obese mice were subjected to oVa sensitization and challenge to develop asthma associated with obesity. airway remodeling was observed in obese mice with asthma through He and Masson staining. Proteomic and bioinformatics analyses were conducted on lung tissue from obese mice with asthma and normal mice. a total of 200 proteins were differentially expressed in obese mice with asthma compared with normal mice; of these, 53 and 47% were up-and downregulated, respectively. Pathway analysis revealed that asthma associated with obesity primarily affected the 'lysosome', 'phagosome', and 'sphingolipid metabolism' pathways. Gene Set enrichment analysis demonstrated the presence of pyroptosis in obese asthmatic mice, along with significant increases in pyroptosisassociated factors such as GSdMd and caspase. High protein expression of orosomucoid-like 3 (orMdl3), nod-like receptor thermal protein domain associated protein 3 (nlrP3) and Gasdermin-d (GSdMd) was observed in obese mice with asthma. In vitro experiments using HBe cells infected with orMdl3-overexpressing lentivirus demonstrated that the overexpression of orMdl3 led to increased expression of NLRP3, GSDMD and cathepsin D (CTSD). These findings suggested that orMdl3 may regulate pyroptosis and subsequent airway remodeling in asthma associated with obesity via the cTSd/nlrP3/GSdMd pathway.

show abstract

The mechanisms, regulations, and functions of histone lysine crotonylation

Xie,

Ju,

Zhou

et al. 2024

Cell Death Discov.

View full text Add to dashboard Cite

Histone lysine crotonylation (Kcr) is a new acylation modification first discovered in 2011, which has important biological significance for gene expression, cell development, and disease treatment. In the past over ten years, numerous signs of progress have been made in the research on the biochemistry of Kcr modification, especially a series of Kcr modification-related “reader”, “eraser”, and “writer” enzyme systems are identified. The physiological function of crotonylation and its correlation with development, heredity, and spermatogenesis have been paid more and more attention. However, the development of disease is usually associated with abnormal Kcr modification. In this review, we summarized the identification of crotonylation modification, Kcr-related enzyme system, biological functions, and diseases caused by abnormal Kcr. This knowledge supplies a theoretical basis for further exploring the function of crotonylation in the future.

show abstract

Folate-deficiency induced acyl-CoA synthetase short-chain family member 2 increases lysine crotonylome involved in neural tube defects

Cited by 6 publications

References 48 publications

Multiplexing of TMT labeling reveals folate-deficient diet-specific proteome changes in NTDs

Multiplexing of TMT labeling reveals folate-deficient diet-specific proteome changes in NTDs

ORMDL3‑mediated bronchial epithelial pyroptosis leads to lung inflammation in obese mice with asthma

The mechanisms, regulations, and functions of histone lysine crotonylation

Contact Info

Product

Resources

About