Folate-mediated targeting of albumin conjugates of paclitaxel obtained through a heterogeneous phase system

Dosio, Franco; Berlier, Gloria; Stella, Barbara; Brusa, Paola; Cattel, Luigi

doi:10.1016/j.ijpharm.2009.08.018

Cited by 41 publications

(29 citation statements)

References 35 publications

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“…11,12 Compared with nontargeted carriers, these new vectors can improve the gene transfection efficiency of FR-positive HEK 293T cells, C6 cells, and KB cells in vitro, 11,12 and have had stronger antitumor effects in experiments in vivo. 13 Chemotherapeutic drugs (eg, paclitaxel, doxorubicin) conjugated with FA have been shown not only to be selectively transported into KB cells, 14,15 Bel 7402 cells, 16 and nude mice xenograft tumors, but also to achieve better antitumor results. Saba et al detected the FR in 45% of primary squamous cell carcinomas of the head and neck and 40% of corresponding lymph node metastases.…”

Section: Introductionmentioning

confidence: 99%

Expression of folate receptors in nasopharyngeal and laryngeal carcinoma and folate receptor-mediated endocytosis by molecular targeted nanomedicine

Xie¹,

Zhang²,

Xu³

et al. 2013

IJN

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Abstract:Immunohistochemistry and an immunofluorescence technique was used to detect folate receptor expression in tissue samples and cell lines of head and neck squamous carcinoma, including 20 tissue samples of nasopharyngeal carcinoma, 16 tissue samples of laryngeal carcinoma, and HNE-1, HNE-2, CNE-1, CNE-2, SUNE-1, 5-8F, and Hep-2 cell lines. Iron staining, electron microscopy, and magnetic resonance imaging were used to observe endocytosis of folate-conjugated cisplatin-loaded magnetic nanoparticles (CDDP-FA-ASA-MNP) in cultured cells and transplanted tumors. As shown by immunohistochemistry, 83.3% (30/36) of the head and neck squamous carcinomas expressed the folate receptor versus none in the control group (0/24). Only the HNE-1 and Hep-2 cell lines expressed the folate receptor, and the other five cell lines did not. Endocytosis of CDDP-FA-ASA-MNP was seen in HNE-1 and Hep-2 cells by iron staining and electron microscopy. A similar result was seen in transplanted tumors in nude mice. Magnetic resonance imaging showed low signal intensity of HNE-1 cells and HNE-1 transplanted tumors on T2-weighted images after uptake of CDDP-FA-ASA-MNP, and this was not seen in CNE-2 transplanted tumors. In conclusion, head and neck squamous carcinoma cell strongly expressed the folate receptor, while normal tissue did not. The folate receptor can mediate endocytosis of folate-conjugated anticancer nanomedicines, and lays the foundation for molecular targeted treatment of cancer.

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Section: Introductionmentioning

confidence: 99%

Expression of folate receptors in nasopharyngeal and laryngeal carcinoma and folate receptor-mediated endocytosis by molecular targeted nanomedicine

Xie¹,

Zhang²,

Xu³

et al. 2013

IJN

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“…Folate could enable binding, promote cell uptake, and thus increase toxicity when 2-3 folate groups were inserted per HSA molecule. 18 Our findings were as follows.…”

mentioning

confidence: 70%

Preparation, characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells

Zu¹

2011

IJN

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Background: The purpose of this study was to develop a method for targeted delivery of 10-hydroxycamptothecin (HCPT)-loaded nanoparticles (NPs) to cancer cells. Methods: We first used a supercritical antisolvent process to prepare micronized HCPT (nHCPT), and then folate-conjugated human serum albumin (HSA) nHCPT-loaded NPs (FA-HSA-nHCPT-NPs) were prepared using a NP-coated method combined with a desolvation technique. The amount of folate conjugation was 16 μg · mg −1 HSA. Results: The particle size of the spherical nHCPT microparticles obtained was 118.5 ± 6.6 nm. The particle size and zeta potential of the FA-HSA-nHCPT-NPs were 233.9 ± 1.2 nm and −25.23 ± 2.98 mV, respectively. The FA-HSA-nHCPT-NPs exhibited a smooth surface and a distinct spherical shape, and the results of differential scanning calorimetry and X-ray diffraction indicated that the FA-HSA-nHCPT-NPs presented in a nanostructured amorphous state. The FA-HSA-nHCPT-NPs showed sustained-release characteristics for 120 hours in vitro, with a drug-loading content of 7.3% and an encapsulating efficiency of 79.1%. Conclusion: The FA-NPs were effective delivery systems for uptake by SGC7901 cells compared with folate-free NPs. These results suggest that a NP-coated method combined with a desolvation technique is effective for preparing NPs with drugs having poor solubility in water and most organic solvents, using albumin as the wall material. FA-HSA-NPs are a stable delivery system and have the potential for targeted delivery of anticancer drugs.

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“…Market approved products include the taxol albumin nanoparticle Abraxane® for treating metastatic breast cancer which is also under clinical investigation in further tumor indications 66,68,71,72 , fatty acid derivatives of human insulin or the glucagon-like-1 peptide (Levemir® and Victoza®) for treating diabetes 66,71 , and 99mTc-aggregated albumin (Nanocoll® and Albures®) for diagnosing cancer and rheumatoid arthritis as well as for lymphoscintigraphy 71 .…”

Section: Albumin Based Drug Targeting In To Tumor Cellsmentioning

confidence: 99%

“…In these cases the albumin-based targeted drug delivery system has increased the disease tissue/normal tissue drug concentration ratio 72 . In addition, some albuminbased or albumin-binding drugs are in clinical trials for treatment of cancers, diabetes, rheumatoid arthritis and vascular diseases as shown in table 1 below 71 .…”

Section: Albumin Based Drug Targeting In To Tumor Cellsmentioning

confidence: 99%

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2013

IJPSR

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Folate-mediated targeting of albumin conjugates of paclitaxel obtained through a heterogeneous phase system

Cited by 41 publications

References 35 publications

Expression of folate receptors in nasopharyngeal and laryngeal carcinoma and folate receptor-mediated endocytosis by molecular targeted nanomedicine

Expression of folate receptors in nasopharyngeal and laryngeal carcinoma and folate receptor-mediated endocytosis by molecular targeted nanomedicine

Preparation, characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells

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