Folate-molecular encapsulator-tethered biocompatible polymer grafted with magnetic nanoparticles for augmented drug delivery

Abstract

Magnetic ferrite nanoparticles (MNPs) coated with biocompatible polymers capable of drug loading and release are fascinating nanostructures for delivering anti-cancer drugs. Herein, we report the synthesis and characterization of a novel β-cyclodextrin-folate-tethered dextran polymer. Nickel-zinc ferrite nanoparticles are prepared and coated with the polymer to form a biocompatible hybrid magnetic nanocarrier. To establish the significance of folate unit of the polymer in anticancer activity, a similar derivatized polymer, i.e. β-cyclodextrin-dextran conjugate without folate tether is used for comparison. The size of the hybrid MNPs is ∼20 nm, which is a size suitable for cancer drug targeting. The polymer-coated magnetic nanocarriers are soft ferromagnets as suggested by their narrow magnetic hysteresis loops. The anticancer drug camptothecin (CPT) is loaded on the magnetic nanocarriers. The drug loading efficiency is observed to be above 92%. The nanocarriers show sustained in vitro drug release for above 45 h. The in vitro cytotoxicity studies reveal that the loaded CPT retains its potency in the nanocarrier and the folate-tethered nanocarrier shows better anticancer activity than the one which does not carry a folate unit. The magnetic nanocarrier is suitable for magnetic field-guided drug transport, enhanced drug loading and release and folate receptor-mediated endocytotic uptake of drugs by cancer cells.

Polysaccharide/Lipid Nanoconjugates as Alternative Building Blocks for Highly Biocompatible Microcapsules

“…Similarly, Blanco-Fernandez et al reported proliferation in HeLa cells after 24 h of incubation with 400 μg/mL of dextran . In addition, a wider range of concentrations was evaluated, finding the IC50 at 2282 μg/mL (Figure B) that is 100 times higher than the value reported for a β-cyclodextrin-folate-tethered dextran conjugate by Ramasamy et al, 2018 (approximately 20 μg/mL), in the same cell line, suggesting that the conjugate fabricated in this research shows high biocompatibility …”

Poly-l-lysine-coated superparamagnetic nanoparticles: a novel method for the transfection of pro-BDNF into neural stem cells

“…Some of non-viral carriers were designed and developed to overcome the challenges of ineffective DNA-endosomal capture and release from the carrier vector into the nucleus, and represent a promising methods for gene delivery purposes [10]. Superparamagnetic iron oxide NPs (SPIONs) are widely used for biomedical applications, such as target gene delivery systems and gene therapy, It can be used also in cancer chemotherapy as drug carrier after coated with biocompatible polymers [11,12] SPIONs with unique magnetic properties are being actively investigated as magnetic resonance imaging contrast agents [12], It can be properly designed and engineered to reach their target tissue with less nonspecific cellular interactions [13,14]. In addition, SPIONs showed a high labeling efficiency in different mammalian cell types [15].…”

Functions and applications of metallic and metallic oxide nanoparticles in orthopedic implants and scaffolds

“…Furthermore, different organic and inorganic materials, such as polymeric nanoparticles, metallic nanoparticles, liposomes, etc., have been studied as drug delivery carriers. 176 Therapeutic agents like drugs, 178 genes, 179 growth factors, 180 and enzymes 181 an external magnetic field. 11,12 To enhance drug delivery efficiency of nanoparticles, multiple parameters ( Figure 6) should also be adjusted:…”

“…Furthermore, different organic and inorganic materials, such as polymeric nanoparticles, metallic nanoparticles, liposomes, etc., have been studied as drug delivery carriers 176 . Therapeutic agents like drugs, 178 genes, 179 growth factors, 180 and enzymes 181 can be loaded on MNPs, especially SPIONs. MNPs are one of the most suitable carriers for delivering bioactive molecules because of their unique advantages, especially the ease with which they can be monitored and visualized through MRI with guidance from an external magnetic field.…”