Folate targeted hybrid lipo-polymeric nanoplexes containing docetaxel and miRNA-34a for breast cancer treatment

Cationic lipopolymeric nanoplexes containing the CRISPR/Cas9 ribonucleoprotein for genome surgery

“…We have previously reported the non-cytotoxic nature of the blank lipopolymeric nanoplexes in vitro in cancer cell lines and in vivo in swiss albino mice. 34 To further test the RNPs lipopolymeric nanoplexes, MTT assay was performed in HEK293T cells wherein the developed RNPs lipopolymeric nanoplexes showed minimal toxicity up to a dose of 20Â of the working concentration and the probable reason for the reduced cytotoxicity could be the reduction in the net charge on the nanoplexes after complexation with the RNPs. The blank nanoplexes are cationic and possess a net positive charge of 16.2 AE 2.42 mV, while upon complexation of RNPs with nanoplexes, the net charge gets reduced to 6.17 AE 1.04 mV.…”

“…2,13,14 We have previously reported that cholesterol and morpholine grafted amphiphilic cationic polymeric nanocarriers efficiently deliver miRNA-34a into the cancer cell by escaping the lysosomal acidic environment. 15,16 These cationic polymers, being positively charged, could be electrostatically complexed with the negatively charged sgRNA/Cas9 RNPs to form stable nanoplexes. Herein, we report a robust gene-editing strategy based on the delivery of these anionic RNPs using cationic amphiphilic lipopolymeric nanoplexes.…”

Advances in Lipid‐Based Codelivery Systems for Cancer and Inflammatory Diseases

“…The hybrid lipopolymeric nanoplexes are a viable delivery strategy for the codelivery of hydrophilic NAs and hydrophobic drugs, and further research is required to realize the translational potential of the delivery system. [103] Shi et al developed SLNs to codeliver miR-34a and PTX and assessed their synergistic efficacy in B16F10-CD44+ cells in vitro and in vivo. The SLNs exhibited enhanced anticancer efficacy by regulating the CD44 expression combined with the cytotoxicity of PTX.…”

MicroRNA-dependent mechanisms of taxane resistance in breast cancer

“…В первом случае авторами был предложен механизм наблюдаемого синергизма: miR-34a-зависимая активация сигнальной цепочки JAG1 / Notch1. В контексте РМЖ комплексы «паклитаксел + miR-34a» были сформированы с помощью наночастиц, стабилизированных альбумином [62], или комплексных липополимерных наночастиц [63]. Обе работы были сфокусированы на создании и оценке фармакологических характеристик наночастиц в условиях in vivo экспериментов, механизмы синергичного действия компонентов терапевтической смеси не изучались.…”