Foliar anatomy of some species of Asteraceae in South Western Nigeria

Mabel, Akinnubi Funmilola; Akinloye, Akinwumi Johnson; Olufemi, Olaleye-Otunla; Ayomipo, Adenegan-Alakinde Taiwo

doi:10.5897/ajps2014.1196

Cited by 13 publications

(4 citation statements)

References 8 publications

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“…As leaves become more adapted in the field and with differentiation of the mesophyll tissues, air spaces narrow and gradually become normal (Shekhawat and Manokari, 2018). The features noted through cross sections of the tissues of A. africana in our study, such as the presence of secretory ducts, uniseriate epidermises, and five (5) vascular bundles (three prominent ones) in leaves, have been previously reported (Mabel et al, 2014;Ekeke and Mensah, 2015).…”

Section: Discussionsupporting

confidence: 79%

An in vitro Propagation of Aspilia africana (Pers.) C. D. Adams, and Evaluation of Its Anatomy and Physiology of Acclimatized Plants

et al. 2021

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Aspilia africana (Pers.) C. D. Adams is an important medicinal plant, that has been used as traditional medicine in many African countries for the treatment of various health problems, including inflammatory conditions, osteoporosis, tuberculosis, cough, measles, diabetes, diarrhea, malaria, and wounds. We developed an efficient and reproducible protocol for in vitro regeneration of A. africana from nodes. We assessed the effects of plant tissue culture media on A. africana growth, cytokinins for in vitro shoot regeneration and proliferation, and auxins for the rooting of regenerated shoots. Furthermore, chlorophyll content, photosynthetic rates, anatomy (leaves, stems, and roots), and Fourier transform near-infrared (FT-NIR) spectra (leaves, stems, and roots) of the in vitro regenerated and maternal A. africana plants were compared. Murashige and Skoog media, containing vitamins fortified with benzylaminopurine (BA, 1.0 mg/l), regenerated the highest number of shoots (13.0 ± 0.424) from A. africana nodal segments. 1-naphthaleneacetic acid (NAA, 0.1 mg/l) produced up to 13.10 ± 0.873 roots, 136.35 ± 4.316 mm length, and was the most efficient for rooting. During acclimatization, the in vitro regenerated A. africana plants had a survival rate of 95.7%, displaying normal morphology and growth features. In vitro regenerated and mother A. africana plants had similar chlorophyll contents, photosynthetic rates, stem and root anatomies, and FT-NIR spectra of the leaf, stem, and roots. The established regeneration protocol could be used for large-scale multiplication of the plant within a short time, thus substantially contributing to its rapid propagation and germplasm preservation, in addition to providing a basis for the domestication of this useful, high-value medicinal plant.

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Section: Discussionsupporting

confidence: 79%

An in vitro Propagation of Aspilia africana (Pers.) C. D. Adams, and Evaluation of Its Anatomy and Physiology of Acclimatized Plants

et al. 2021

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“…To the best of our knowledge, this is the first histological study of A. africana through its different stages of development. Previous histological studies of A. africana have focused on the microanatomical features of mature plant leaf and stem features (Mabel et al, 2014;Ekeke and Mensah, 2015).…”

Section: Introductionmentioning

confidence: 99%

Indirect in vitro Regeneration of the Medicinal Plant, Aspilia africana, and Histological Assessment at Different Developmental Stages

et al. 2021

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The medicinal plant, Aspilia africana, has been traditionally used in several African countries to treat many diseases such as tuberculosis, cough, inflammation, malaria, osteoporosis, and diabetes. In this study, we developed a protocol for in vitro propagation of A. africana using indirect shoot organogenesis from leaf and root explants of in vitro-grown seedlings and assessed the tissues at different developmental stages. The highest callus induction (91.9 ± 2.96%) from leaf explants was in the Murashige and Skoog (MS) medium augmented with 1.0 mg/L 6-Benzylaminopurine (BAP) and 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) while from root explants, the highest callus induction (92.6 ± 2.80%) was in the same plant tissue culture medium augmented with 0.5 mg/L BAP and 1.0 mg/L 2,4-D. The best shoot regeneration capacity from leaf-derived calli (i.e., 80.0 ± 6.23% regeneration percentage and 12.0 ± 6.23 shoots per callus) was obtained in medium augmented with 1.0 mg/L BAP and 0.05 mg/L α-Naphthaleneacetic acid (NAA); the best regeneration capacity for root-derived calli (i.e., 86.7 ± 6.24% shoot regeneration percentage and 14.7 ± 1.11 shoots per callus) was obtained in the MS medium augmented with 1.0 mg/L BAP, 0.05 mg/L NAA, and 0.1 mg/L Thidiazuron (TDZ). Regenerated plantlets developed a robust root system in 1/2 MS medium augmented with 0.1 mg/L NAA and had a survival rate of 93.6% at acclimatization. The in vitro regenerated stem tissue was fully differentiated, while the young leaf tissue consisted of largely unorganized and poorly differentiated cells with large intercellular airspaces typical of in vitro leaf tissues. Our study established a protocol for the indirect regeneration of A. africana and offers a basis for its domestication, large-scale multiplication, and germplasm preservation. To the best of our knowledge, this is the first study to develop an indirect regeneration protocol for A. africana and conduct anatomical assessment through the different stages of development from callus to a fully developed plantlet.

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“…The information obtained through comparative morpho-anatomical studies is necessary for ecological, phylogenetic and evolutionary studies. For example, anatomical characters, especially of leaves, have been extensively used in the systematics of several plant families, including Bromeliaceae (De Faria et al, 2012), Myrtaceae (Al-Edany and Al-Saadi, 2012), Amaryllidaceae (Lin and Tan, 2015), Malpighiaceae (Araújo et al, 2010) and Asteraceae (Castro et al, 1997;Milan et al, 2006;Adedeji and Jewoola, 2008;Bombo et al, 2012;Akinnubi et al, 2014;Rojas-Leal et al, 2017;Lusa et al, 2018). Particularly in Asteraceae, leaf architecture and anatomical characters are extremely diverse (Bombo et al, 2012;Rojas-Leal et al, 2014, 2018 and have been studied from an ecological (Bercu et al, 2012;Moroney et al, 2013;Rivera et al, 2017;Ferraro and Scremin-Dias, 2018), physiological (Bondarev et al, 2003;McKown and Dengler, 2007;Santiago and Kim, 2009), and medical perspective (Cambi et al, 2006;Hulley et al, 2010;García-Sánchez et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Leaf architecture and anatomy of Asteraceae species in a xerophytic scrub in Mexico City, Mexico

et al. 2019

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Background and Aims: Leaf architecture and anatomy in the Asteraceae family are extremely diverse and have been studied from ecological, physiological and evolutionary perspectives. The aims of this study are to describe in detail leaf architecture and anatomy for 61 species belonging to 13 tribes of Asteraceae inhabiting a xerophytic scrub in Mexico City, Mexico and to discuss characters common to these tribes.Methods: Mature and undamaged leaves of 61 species of Asteraceae were collected in southwestern Mexico City in the “Reserva Ecológica del Pedregal de San Ángel” (REPSA). Standard anatomical techniques were used to obtain permanent slides of cleared leaves and transverse and paradermal sections. The permanent slides were analyzed to describe leaf architecture and anatomy by tribe following the standard terminologies.Key results: The results show a significant variation in leaf architecture although pinnate venation, brochidodromous secondary venation, areoles moderately developed and looped ultimate marginal venation predominate in the material studied. For anatomy, the most common traits are the striate cuticle, occurrence of trichomes and glands, as well as collateral vascular bundles with a parenchymatous sheath with girders in the Asteraceae present in this xerophytic scrub. There are no unique combinations of leaf characters for the family or any tribe.Conclusions: Leaf diversity in the family and within each tribe is consistent with some previous reports. Anatomical descriptions are a fundamental piece of the evolutionary, ecological and physiological studies in Asteraceae. The results of this descriptive study will allow testing hypotheses about the factors causing leaf diversity in this plant lineage. More leaf anatomical studies of the family are necessary to confirm the patterns proposed for the tribes and the family.

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Foliar anatomy of some species of Asteraceae in South Western Nigeria

Cited by 13 publications

References 8 publications

An in vitro Propagation of Aspilia africana (Pers.) C. D. Adams, and Evaluation of Its Anatomy and Physiology of Acclimatized Plants

An in vitro Propagation of Aspilia africana (Pers.) C. D. Adams, and Evaluation of Its Anatomy and Physiology of Acclimatized Plants

Indirect in vitro Regeneration of the Medicinal Plant, Aspilia africana, and Histological Assessment at Different Developmental Stages

Leaf architecture and anatomy of Asteraceae species in a xerophytic scrub in Mexico City, Mexico

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